FastQCFastQC Report
Thu 2 Feb 2017
SRR4062324_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062324_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1340465
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT25190.18791986362941218No Hit
TATCAACGCAGAGTACTTTTTTTTT16480.12294241177501837No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGTA400.00527783814.2503099
CGCGGTA500.001500380313.29879817
TAGCGCG604.0906205E-412.6669414
CGCGCGT604.0906205E-412.6669417
CTAGACT853.941208E-612.2943834
TCCAACG1353.7107384E-1011.96143418
ACCGTGC1052.7191345E-711.761728
AACGCCG658.0181786E-411.6929975
CGCGTGC658.020692E-411.692569
GATATAC3500.011.6673231
GTCCTAC4200.011.5316561
GTATCAA10800.011.4311631
GTCTAGG1251.8399078E-811.395991
GGTATCA4850.011.3568291
TTAGGAC2200.011.2258393
GTAGGAC4850.011.1635223
TAGGACT1201.2740202E-711.0835734
TGAGTTA3450.011.016376512
AATCCCG2600.010.95931919
TAGGACG3050.010.90187554
Produced by FastQC (version 0.11.5)