##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062324_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1340465
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.23690361180635	32.0	32.0	32.0	32.0	32.0
2	30.814001857564353	32.0	32.0	32.0	32.0	32.0
3	30.829541987295453	32.0	32.0	32.0	32.0	32.0
4	30.833621914783304	32.0	32.0	32.0	32.0	32.0
5	30.774565542554264	32.0	32.0	32.0	32.0	32.0
6	34.40146367118873	36.0	36.0	36.0	32.0	36.0
7	34.35995270297994	36.0	36.0	36.0	32.0	36.0
8	34.308965918543194	36.0	36.0	36.0	32.0	36.0
9	34.427438985725104	36.0	36.0	36.0	32.0	36.0
10	34.14787853468759	36.0	36.0	36.0	32.0	36.0
11	34.41031955328934	36.0	36.0	36.0	32.0	36.0
12	34.26330489792721	36.0	36.0	36.0	32.0	36.0
13	34.32424494485123	36.0	36.0	36.0	32.0	36.0
14	34.245513310679506	36.0	36.0	36.0	32.0	36.0
15	34.20307952837262	36.0	36.0	36.0	32.0	36.0
16	34.20823370994394	36.0	36.0	36.0	32.0	36.0
17	34.14830898233076	36.0	36.0	36.0	32.0	36.0
18	34.15652628005953	36.0	36.0	36.0	32.0	36.0
19	34.15243889247388	36.0	36.0	36.0	32.0	36.0
20	34.13988429388309	36.0	36.0	36.0	32.0	36.0
21	34.11532266788018	36.0	36.0	36.0	32.0	36.0
22	34.08952863372039	36.0	36.0	36.0	32.0	36.0
23	34.046028803437615	36.0	36.0	36.0	32.0	36.0
24	34.03000376734939	36.0	36.0	36.0	32.0	36.0
25	33.679181478069175	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	17.0
5	100.0
6	285.0
7	63.0
8	229.0
9	187.0
10	102.0
11	29.0
12	46.0
13	60.0
14	150.0
15	248.0
16	421.0
17	572.0
18	788.0
19	992.0
20	1557.0
21	2433.0
22	3439.0
23	5273.0
24	7532.0
25	10739.0
26	15006.0
27	19604.0
28	26043.0
29	35021.0
30	46966.0
31	64258.0
32	90903.0
33	127668.0
34	276325.0
35	603409.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.91454362341005	17.352008653810287	11.618411727404975	26.115035995374686
2	16.30532250917224	19.362188147633123	38.278856928005204	26.053632415189437
3	19.77670202043939	22.35329750956954	28.35941037755203	29.51059009243904
4	13.132735968007877	15.28093827639462	35.345549230413404	36.240776525184096
5	14.833247513078259	36.145849657838376	33.941612376027045	15.079290453056322
6	34.83282869905881	34.84492200563757	16.348657493669677	13.973591801633939
7	30.112617994981477	30.114260963078028	20.257049826741547	19.51607121519895
8	27.164274965366076	34.581872360446745	19.22554434076049	19.028308333426686
9	26.454415903346657	15.004018259929136	18.81807888337187	39.723486953352335
10	15.94591990175404	27.654086373079174	32.00097708117169	24.399016643995097
11	36.72263584029213	21.4912811502218	22.237437191560698	19.548645817925376
12	23.799254345109283	23.616090070210483	29.604486180135943	22.980169404544295
13	29.568075292711622	19.955406995200835	25.066646747959886	25.409870964127656
14	22.621916926273617	19.75026944101912	25.648693724545012	31.97911990816225
15	24.624260098663473	27.794145329919058	22.37333158037846	25.208262991039014
16	24.866954629754808	26.41166272914614	24.47272952707179	24.248653114027256
17	23.4627194767887	26.582208515180284	25.84909423226166	24.10597777576936
18	23.76819798753205	26.219516293701123	26.578976259808968	23.433309458957858
19	25.011163764585703	25.47638285066542	25.547696530794816	23.964756853954064
20	24.92338755443516	25.66987353719512	25.60767259454842	23.799066313821303
21	24.979167102240993	25.151878544952766	25.404860113438488	24.464094239367757
22	24.869800806809337	25.33645681957013	25.521506203410944	24.272236170209588
23	24.37597434013654	25.20800855328782	25.68233674389712	24.733680362678516
24	24.292799412196914	25.29957647115046	25.842353482315133	24.565270634337498
25	24.504763085230778	25.24763712443756	25.836545310910967	24.411054479420695
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	238.0
1	238.0
2	344.5
3	451.0
4	451.0
5	451.0
6	1045.0
7	1639.0
8	1639.0
9	1639.0
10	2054.0
11	2469.0
12	2469.0
13	2469.0
14	3330.0
15	4191.0
16	4191.0
17	4191.0
18	7065.0
19	9939.0
20	9939.0
21	9939.0
22	15262.0
23	20585.0
24	20585.0
25	20585.0
26	30242.0
27	39899.0
28	39899.0
29	39899.0
30	52419.5
31	64940.0
32	64940.0
33	64940.0
34	76450.0
35	87960.0
36	87960.0
37	87960.0
38	101782.0
39	115604.0
40	115604.0
41	115604.0
42	130546.0
43	145488.0
44	145488.0
45	145488.0
46	157307.0
47	169126.0
48	169126.0
49	169126.0
50	174347.5
51	179569.0
52	179569.0
53	179569.0
54	170521.0
55	161473.0
56	161473.0
57	161473.0
58	145973.5
59	130474.0
60	130474.0
61	130474.0
62	112472.0
63	94470.0
64	94470.0
65	94470.0
66	76286.0
67	58102.0
68	58102.0
69	58102.0
70	43692.0
71	29282.0
72	29282.0
73	29282.0
74	21406.5
75	13531.0
76	13531.0
77	13531.0
78	10078.5
79	6626.0
80	6626.0
81	6626.0
82	4577.0
83	2528.0
84	2528.0
85	2528.0
86	1751.5
87	975.0
88	975.0
89	975.0
90	628.5
91	282.0
92	282.0
93	282.0
94	199.0
95	116.0
96	116.0
97	116.0
98	312.0
99	508.0
100	508.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0011190146702823275
2	5.222068461317529E-4
3	0.0010444136922635057
4	0.010071132032540947
5	0.03394344499856393
6	0.06572346163458204
7	0.10630639367682111
8	0.10891742790747987
9	0.11764574233568202
10	0.13308814478557815
11	0.13957842987321564
12	0.13249133696142756
13	0.12607565285180888
14	0.11801874722577614
15	0.1322675340273711
16	0.12204720003879252
17	0.12353921959916896
18	0.1127966787644586
19	0.09780188218267542
20	0.09377342936965903
21	0.0922814098092826
22	0.10168113303965416
23	0.08183727288664755
24	0.09257981372135789
25	0.09004338046871795
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1340465.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.82625812563126
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.54067787049613	44.594898351398314
2	15.032469334050141	17.9867278128904
3	4.920182293369537	8.830682877248591
4	2.045306738211189	4.894521954652621
5	1.0462982818513993	3.1298055543223153
6	0.6009395131156152	2.157117745772758
7	0.3809374214396151	1.5953042353331157
8	0.2674739060315164	1.2801570355289873
9	0.19337338682476102	1.0411925539325126
>10	0.9027758420399984	9.29725134517831
>50	0.04525613427231972	1.8956069197300973
>100	0.02255503091678321	2.358030321770608
>500	0.0013783372279210235	0.5476144026371195
>1k	3.759101530693701E-4	0.3910888896040865
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	2519	0.18791986362941218	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1648	0.12294241177501837	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	7.460097801882182E-5	0.0
10	0.0	0.0	0.0	7.460097801882182E-5	0.0
11	0.0	0.0	0.0	7.460097801882182E-5	0.0
12	0.0	0.0	0.0	7.460097801882182E-5	0.0
13	0.0	0.0	0.0	7.460097801882182E-5	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCGTA	40	0.005277838	14.250309	9
CGCGGTA	50	0.0015003803	13.298798	17
TAGCGCG	60	4.0906205E-4	12.666941	4
CGCGCGT	60	4.0906205E-4	12.666941	7
CTAGACT	85	3.941208E-6	12.294383	4
TCCAACG	135	3.7107384E-10	11.961434	18
ACCGTGC	105	2.7191345E-7	11.76172	8
AACGCCG	65	8.0181786E-4	11.692997	5
CGCGTGC	65	8.020692E-4	11.69256	9
GATATAC	350	0.0	11.667323	1
GTCCTAC	420	0.0	11.531656	1
GTATCAA	1080	0.0	11.431163	1
GTCTAGG	125	1.8399078E-8	11.39599	1
GGTATCA	485	0.0	11.356829	1
TTAGGAC	220	0.0	11.225839	3
GTAGGAC	485	0.0	11.163522	3
TAGGACT	120	1.2740202E-7	11.083573	4
TGAGTTA	345	0.0	11.0163765	12
AATCCCG	260	0.0	10.959319	19
TAGGACG	305	0.0	10.9018755	4
>>END_MODULE