FastQCFastQC Report
Thu 2 Feb 2017
SRR4062324_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062324_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1340465
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA11500.022.963571
GCGATTG500.002579897422.00100739
GATATAC3750.021.7133311
GTAGGAC5400.019.9616413
GGTATCA6650.019.1938611
TAGGACG3250.018.9525934
TAGTACT1203.164805E-718.332124
CTAGCGG1601.1004886E-917.87581829
TGTAGGA6750.017.5994912
TAGCGGC1651.6589183E-917.33412730
TGCACCG1802.8558134E-1017.1099785
CGCAATA1551.4386387E-817.03303736
GCGCAAT1551.4393663E-817.03240235
GTCCTAC4800.016.963541
CTTAGAC1501.8393985E-716.1322673
AACCGTG1105.625157E-515.9989417
TTCTCGC2500.015.84131642
ATAGGGG1259.447649E-615.8389523
ATACACT5650.015.5741924
TCCTACA6600.015.3328912
Produced by FastQC (version 0.11.5)