FastQCFastQC Report
Thu 2 Feb 2017
SRR4062323_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062323_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences269783
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT21760.8065741725757368No Hit
TATCAACGCAGAGTACTTTTTTTTT13790.5111515551387597No Hit
GGTATCAACGCAGAGTACTTTTTTT9160.33953214249971275No Hit
ACGCAGAGTACTTTTTTTTTTTTTT8910.33026543555375987No Hit
GTACTTTTTTTTTTTTTTTTTTTTT7840.29060392982508165No Hit
GTACATGGGGTGGTATCAACGCAAA7380.27355318904452836No Hit
GTATCAACGCAGAGTACATGGGGTG6780.25131309237424154No Hit
GTCCTAAAGTGTGTATTTCTCATTT6240.23129700537098336No Hit
GAGTACTTTTTTTTTTTTTTTTTTT6130.22721965431476407No Hit
GTCCTACAGTGGACATTTCTAAATT5890.21832361564664932No Hit
CCATAGGGTCTTCTCGTCTTATTAT5880.2179529473688112No Hit
GATATACACTGTTCTACAAATCCCG5290.19608351897636248No Hit
CTTTAGGACGTGAAATATGGCGAGG5090.1886701534196002No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA4840.17940344647364734No Hit
CTGTAGGACGTGGAATATGGCAAGA4830.17903277819580923No Hit
TATCAACGCAGAGTACATGGGGTGG4650.17236074919472316No Hit
GGTATCAACGCAGAGTACATGGGGT4450.16494738363796088No Hit
GTGTATATCAATGAGTTACAATGAG3910.1449312966347027No Hit
GTATTAGAGGCACTGCCTGCCCAGT3700.1371472628001023No Hit
ACGCAGAGTACATGGGGTGGTATCA3640.13492325313307363No Hit
GTCAGGATACCGCGGCCGTTAAACT3520.13047523379901624No Hit
GCAGAGTACTTTTTTTTTTTTTTTT3500.12973389724334003No Hit
GTCCTAGACAGTCCTTGAAAAACCG3320.12306186824225396No Hit
GTACATGGGTGGTATCAACGCAAAA3310.12269119996441585No Hit
TTGTAGAACAGTGTATATCAATGAG3300.12232053168657774No Hit
GTCCTACAGTGTGCATTTCTCATTT3230.11972585374171094No Hit
ACCTATAACTTCTCTGTTAACCCAA3070.1137951612963011No Hit
CTCTAATACTTGTAATGCTAGAGGT2930.10860580540656752No Hit
GCCTAAAGGAAAGATCCAAAAAGAT2850.10564045918386258No Hit
CCATTGGGATGTCCTGATCCAACAT2830.10489912262818636No Hit
GTTCATGCTAGTCCCTAATTAAGGA2780.10304578123899578No Hit
GACTATAGGCAATAATCACACTATA2700.10008043501629088No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATGCA250.006018061719.00074415
TGCCTCG250.006018061719.00074412
CAATTAG350.002168750416.2803061
TAGGACC1200.015.0422554
AAATCGG456.734689E-414.77835514
TGAGCAG456.734689E-414.7783555
TGCAGTG400.00526275614.2505585
CAACCAA400.005269245214.24791218
TGCAGGA400.005269245214.2479122
CTAGGAC903.592868E-813.7227583
AAGATCC500.00149141113.3029911
AGAAATG500.001493500713.3005215
AGCTCGT655.4084914E-513.15680311
GATGCAA655.427382E-513.15191716
CGTACTC604.0711975E-412.6671623
GCCCAGT751.47008395E-512.6648119
ACTGCCT853.9075367E-612.29459812
AGGCACT853.9075367E-612.2945988
GATATAC1551.8189894E-1212.2539931
TAACCGC701.0821826E-412.217031511
Produced by FastQC (version 0.11.5)