##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062323_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	269783
Sequences flagged as poor quality	0
Sequence length	25
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.90206944099517	32.0	32.0	32.0	32.0	32.0
2	30.670149712917418	32.0	32.0	32.0	32.0	32.0
3	30.734234551472852	32.0	32.0	32.0	32.0	32.0
4	30.671072676929235	32.0	32.0	32.0	32.0	32.0
5	30.783374045065848	32.0	32.0	32.0	32.0	32.0
6	34.30891864943306	36.0	36.0	36.0	32.0	36.0
7	34.173780408698846	36.0	36.0	36.0	32.0	36.0
8	34.035017032207364	36.0	36.0	36.0	32.0	36.0
9	34.043101307347015	36.0	36.0	36.0	32.0	36.0
10	33.93585956120289	36.0	36.0	36.0	32.0	36.0
11	34.22304222282353	36.0	36.0	36.0	32.0	36.0
12	34.04183362183681	36.0	36.0	36.0	32.0	36.0
13	34.12739868709296	36.0	36.0	36.0	32.0	36.0
14	34.06985985032415	36.0	36.0	36.0	32.0	36.0
15	34.06536735079675	36.0	36.0	36.0	32.0	36.0
16	34.06850320442726	36.0	36.0	36.0	32.0	36.0
17	33.994995978249186	36.0	36.0	36.0	32.0	36.0
18	34.05814673274447	36.0	36.0	36.0	32.0	36.0
19	33.957410214876404	36.0	36.0	36.0	32.0	36.0
20	33.819647642735085	36.0	36.0	36.0	32.0	36.0
21	33.79374163679698	36.0	36.0	36.0	32.0	36.0
22	33.80234855420838	36.0	36.0	36.0	32.0	36.0
23	33.86934684542762	36.0	36.0	36.0	32.0	36.0
24	33.85352672332949	36.0	36.0	36.0	32.0	36.0
25	33.557459143089076	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	7.0
5	13.0
6	66.0
7	13.0
8	50.0
9	41.0
10	25.0
11	4.0
12	9.0
13	13.0
14	138.0
15	183.0
16	257.0
17	293.0
18	406.0
19	574.0
20	834.0
21	1167.0
22	1455.0
23	1819.0
24	2339.0
25	2830.0
26	3574.0
27	4134.0
28	5353.0
29	6693.0
30	8431.0
31	11390.0
32	15724.0
33	22341.0
34	52117.0
35	127490.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.42755895588225	21.274900103047692	12.417617448420554	22.87992349264951
2	14.413489409968047	21.788332802040166	42.19555048149988	21.602627306491907
3	17.79881533979287	27.472181242632942	30.265177068892662	24.46382634868153
4	10.41440386119565	17.955152894599294	39.61877365519849	32.011669589006566
5	10.946231057024521	40.767025966754545	35.08793396887549	13.198809007345444
6	27.791605616039465	41.12580447725207	18.325203553610176	12.75738635309828
7	26.18807288380896	34.650981556388466	21.038334508479608	18.122611051322966
8	24.851464939788098	37.204089584918265	19.654871690200952	18.289573785092685
9	25.996511282660332	15.31584026128266	21.469714964370546	37.21793349168646
10	15.700154043169206	28.63903787977209	32.415137061301756	23.245671015756947
11	32.30197560378045	24.657183372558336	25.053269286451414	17.987571737209805
12	23.461175824257406	27.741523567711234	30.710409288996786	18.086891319034574
13	27.177943485377686	23.292516804804446	27.777385985294174	21.752153724523694
14	20.534839533295727	24.146082593593206	27.843422497996023	27.475655375115043
15	22.921824599954714	31.916022968965173	24.615915696325633	20.54623673475448
16	21.19382267468833	29.727914132060558	28.054053452198474	21.02420974105264
17	18.58050053259994	29.9803661763778	28.526572468851253	22.912560822171006
18	18.50913098821006	29.285589701150787	32.10485885099103	20.10042045964812
19	22.659151710345185	27.594830560987283	28.724671344343562	21.02134638432397
20	22.57709046790417	28.652312806794278	29.450395330976065	19.32020139432549
21	23.25906687691309	26.797143122159355	28.04081254058065	21.902977460346907
22	22.12421890074659	29.188250486099772	28.498805159336825	20.18872545381681
23	21.536235647641483	28.8416093190629	29.50232791081597	20.11982712247964
24	21.980060626973437	28.588178111213765	29.41113176534319	20.02062949646961
25	21.26494356508382	29.126872815512368	30.263362942184823	19.344820677218994
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	682.0
1	682.0
2	584.0
3	486.0
4	486.0
5	486.0
6	1003.0
7	1520.0
8	1520.0
9	1520.0
10	1498.5
11	1477.0
12	1477.0
13	1477.0
14	1607.5
15	1738.0
16	1738.0
17	1738.0
18	2953.5
19	4169.0
20	4169.0
21	4169.0
22	6256.0
23	8343.0
24	8343.0
25	8343.0
26	12862.5
27	17382.0
28	17382.0
29	17382.0
30	21641.5
31	25901.0
32	25901.0
33	25901.0
34	27180.0
35	28459.0
36	28459.0
37	28459.0
38	29067.5
39	29676.0
40	29676.0
41	29676.0
42	29976.0
43	30276.0
44	30276.0
45	30276.0
46	32918.5
47	35561.0
48	35561.0
49	35561.0
50	33146.5
51	30732.0
52	30732.0
53	30732.0
54	25559.0
55	20386.0
56	20386.0
57	20386.0
58	17311.0
59	14236.0
60	14236.0
61	14236.0
62	11378.5
63	8521.0
64	8521.0
65	8521.0
66	6730.5
67	4940.0
68	4940.0
69	4940.0
70	3760.0
71	2580.0
72	2580.0
73	2580.0
74	2044.5
75	1509.0
76	1509.0
77	1509.0
78	1118.0
79	727.0
80	727.0
81	727.0
82	491.0
83	255.0
84	255.0
85	255.0
86	175.5
87	96.0
88	96.0
89	96.0
90	58.5
91	21.0
92	21.0
93	21.0
94	16.5
95	12.0
96	12.0
97	12.0
98	55.0
99	98.0
100	98.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0018533413891905718
2	3.7066827783811433E-4
3	3.7066827783811433E-4
4	0.008154702112438515
5	0.03410148156110652
6	0.07339231901194665
7	0.1160191709633298
8	0.11787251235252036
9	0.12713921929847322
10	0.1401126090228072
11	0.14715530630173138
12	0.14418996007902646
13	0.13566458968874984
14	0.11824318063035848
15	0.139371272467131
16	0.12825122413198756
17	0.12973389724334003
18	0.11713117579684412
19	0.10230444468331955
20	0.09711508879358595
21	0.0956324156822335
22	0.10786446885089128
23	0.08451236734709007
24	0.09859776190493841
25	0.09970976673845276
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	269783.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.90113906361779
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.3013671109861	24.46595967870474
2	15.391801352043636	11.359500042626852
3	6.560324651190823	7.262503567682174
4	3.4604682932709214	5.107808868609216
5	1.9818589093246815	3.6566425608729984
6	1.2445631974927926	2.755547977448542
7	0.888973712494852	2.2962899812071185
8	0.6077164927224694	1.7940344647364734
9	0.4439846112121182	1.4745184092400188
>10	2.7000693098148725	19.00861062409418
>50	0.25112251765391297	6.4826175111107815
>100	0.1546914708748104	10.25342590155792
>500	0.011049390776772172	2.7648146843944947
>1k	0.0020089801412313036	1.3177257277144965
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	2176	0.8065741725757368	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1379	0.5111515551387597	No Hit
GGTATCAACGCAGAGTACTTTTTTT	916	0.33953214249971275	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	891	0.33026543555375987	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	784	0.29060392982508165	No Hit
GTACATGGGGTGGTATCAACGCAAA	738	0.27355318904452836	No Hit
GTATCAACGCAGAGTACATGGGGTG	678	0.25131309237424154	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	624	0.23129700537098336	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	613	0.22721965431476407	No Hit
GTCCTACAGTGGACATTTCTAAATT	589	0.21832361564664932	No Hit
CCATAGGGTCTTCTCGTCTTATTAT	588	0.2179529473688112	No Hit
GATATACACTGTTCTACAAATCCCG	529	0.19608351897636248	No Hit
CTTTAGGACGTGAAATATGGCGAGG	509	0.1886701534196002	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	484	0.17940344647364734	No Hit
CTGTAGGACGTGGAATATGGCAAGA	483	0.17903277819580923	No Hit
TATCAACGCAGAGTACATGGGGTGG	465	0.17236074919472316	No Hit
GGTATCAACGCAGAGTACATGGGGT	445	0.16494738363796088	No Hit
GTGTATATCAATGAGTTACAATGAG	391	0.1449312966347027	No Hit
GTATTAGAGGCACTGCCTGCCCAGT	370	0.1371472628001023	No Hit
ACGCAGAGTACATGGGGTGGTATCA	364	0.13492325313307363	No Hit
GTCAGGATACCGCGGCCGTTAAACT	352	0.13047523379901624	No Hit
GCAGAGTACTTTTTTTTTTTTTTTT	350	0.12973389724334003	No Hit
GTCCTAGACAGTCCTTGAAAAACCG	332	0.12306186824225396	No Hit
GTACATGGGTGGTATCAACGCAAAA	331	0.12269119996441585	No Hit
TTGTAGAACAGTGTATATCAATGAG	330	0.12232053168657774	No Hit
GTCCTACAGTGTGCATTTCTCATTT	323	0.11972585374171094	No Hit
ACCTATAACTTCTCTGTTAACCCAA	307	0.1137951612963011	No Hit
CTCTAATACTTGTAATGCTAGAGGT	293	0.10860580540656752	No Hit
GCCTAAAGGAAAGATCCAAAAAGAT	285	0.10564045918386258	No Hit
CCATTGGGATGTCCTGATCCAACAT	283	0.10489912262818636	No Hit
GTTCATGCTAGTCCCTAATTAAGGA	278	0.10304578123899578	No Hit
GACTATAGGCAATAATCACACTATA	270	0.10008043501629088	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGATGCA	25	0.0060180617	19.000744	15
TGCCTCG	25	0.0060180617	19.000744	12
CAATTAG	35	0.0021687504	16.280306	1
TAGGACC	120	0.0	15.042255	4
AAATCGG	45	6.734689E-4	14.778355	14
TGAGCAG	45	6.734689E-4	14.778355	5
TGCAGTG	40	0.005262756	14.250558	5
CAACCAA	40	0.0052692452	14.247912	18
TGCAGGA	40	0.0052692452	14.247912	2
CTAGGAC	90	3.592868E-8	13.722758	3
AAGATCC	50	0.001491411	13.30299	11
AGAAATG	50	0.0014935007	13.300521	5
AGCTCGT	65	5.4084914E-5	13.156803	11
GATGCAA	65	5.427382E-5	13.151917	16
CGTACTC	60	4.0711975E-4	12.667162	3
GCCCAGT	75	1.47008395E-5	12.66481	19
ACTGCCT	85	3.9075367E-6	12.294598	12
AGGCACT	85	3.9075367E-6	12.294598	8
GATATAC	155	1.8189894E-12	12.253993	1
TAACCGC	70	1.0821826E-4	12.2170315	11
>>END_MODULE