FastQCFastQC Report
Thu 2 Feb 2017
SRR4062323_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062323_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences269783
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9070.3361961279991697No Hit
CCATAGGGTCTTCTCGTCTTATTATTATATTTCAGCCTCTTCACTGAAAG6330.23463301987152635No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5310.1968248555320387No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4240.15716334980336047No Hit
GTATTAGAGGCACTGCCTGCCCAGTGACTAAAGTTTAACGGCCGCGGTAT4040.1497499842465982No Hit
ACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA3370.12491520963144452No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA3340.1238032047979302No Hit
CCATTGGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTAATTGTCGA3320.12306186824225396No Hit
GTCAGGATACCGCGGCCGTTAAACTTTAGTCACTGGGCAGGCAGTGCCTC3110.11527783440765356No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA3000.1112004833514343No Hit
GACTATAGGCAATAATCACACTATAAATAATCCACCTATAACTTCTCTGT2980.11045914679575806No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA2960.10971781024008184No Hit
CTCTAATACTTGTAATGCTAGAGGTGATGTTTTTGGTAAACAGGCGGGGT2900.10749380057305316No Hit
GTTCATGCTAGTCCCTAATTAAGGAACAAGTGATTATGCTACCTTTGCAC2870.1063817957395388No Hit
GTCCTAGACAGTCCTTGAAAAACCGACGGAACGTATTACTCTCATGCCTG2870.1063817957395388No Hit
CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA2860.10601112746170069No Hit
CACTATAAATAATCCACCTATAACTTCTCTGTTAACCCAACACCGGAATG2770.10267511296115767No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGAGG207.848524E-443.996854
ACCGACG301.2990033E-436.6640422
CGACGGA250.002348253335.20400224
CGGATGC451.0338317E-634.21977214
CGGAACG300.00573401629.3366727
GACGGAA300.00573401629.3366725
CCGACGG300.00573401629.3366723
ACGAAAG554.9450737E-627.99799322
TTAATCC407.0132135E-427.5031341
GATGCAA1250.026.3981116
CGAAAGT609.707794E-625.66958623
GGATGCA609.721776E-625.6648315
ATACATG959.513315E-1025.4813081
ATGCAAC1400.025.14105617
TTTCCCC450.001395985424.44269410
CCGGATG651.807628E-523.69060913
TTTCAAT752.055669E-623.46498734
TGCAACG852.3402208E-723.2924518
GTCCTAC3650.022.9109631
CCTACAG3600.022.6094913
Produced by FastQC (version 0.11.5)