##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062323_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	269783
Sequences flagged as poor quality	0
Sequence length	50
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.01957869843541	32.0	32.0	32.0	32.0	32.0
2	31.37814095031933	32.0	32.0	32.0	32.0	32.0
3	31.544196632108026	32.0	32.0	32.0	32.0	32.0
4	31.644651442084935	32.0	32.0	32.0	32.0	32.0
5	31.63513268071005	32.0	32.0	32.0	32.0	32.0
6	35.226011275729014	36.0	36.0	36.0	36.0	36.0
7	35.21962836798464	36.0	36.0	36.0	36.0	36.0
8	35.182294659040785	36.0	36.0	36.0	36.0	36.0
9	35.21736729148983	36.0	36.0	36.0	36.0	36.0
10	35.15189986025806	36.0	36.0	36.0	36.0	36.0
11	35.25745135905524	36.0	36.0	36.0	36.0	36.0
12	35.18091206636445	36.0	36.0	36.0	36.0	36.0
13	35.23176775408384	36.0	36.0	36.0	36.0	36.0
14	35.18708369319045	36.0	36.0	36.0	36.0	36.0
15	35.19382614916433	36.0	36.0	36.0	36.0	36.0
16	35.1829952220859	36.0	36.0	36.0	36.0	36.0
17	35.171230210947314	36.0	36.0	36.0	36.0	36.0
18	35.16733448734724	36.0	36.0	36.0	36.0	36.0
19	35.115663329416606	36.0	36.0	36.0	36.0	36.0
20	35.07369997368255	36.0	36.0	36.0	36.0	36.0
21	35.05751659667214	36.0	36.0	36.0	36.0	36.0
22	35.06138266680999	36.0	36.0	36.0	36.0	36.0
23	35.07768836435209	36.0	36.0	36.0	36.0	36.0
24	35.06184229547451	36.0	36.0	36.0	36.0	36.0
25	35.045206703165135	36.0	36.0	36.0	36.0	36.0
26	35.03410889492666	36.0	36.0	36.0	36.0	36.0
27	35.01817757234518	36.0	36.0	36.0	36.0	36.0
28	34.97171801040095	36.0	36.0	36.0	36.0	36.0
29	34.94385116927308	36.0	36.0	36.0	36.0	36.0
30	34.92027666680258	36.0	36.0	36.0	36.0	36.0
31	34.874854976036296	36.0	36.0	36.0	36.0	36.0
32	34.83927082136384	36.0	36.0	36.0	32.0	36.0
33	34.794597880518786	36.0	36.0	36.0	32.0	36.0
34	34.75611880659641	36.0	36.0	36.0	32.0	36.0
35	34.7331633201499	36.0	36.0	36.0	32.0	36.0
36	34.68866459339543	36.0	36.0	36.0	32.0	36.0
37	34.64191220351171	36.0	36.0	36.0	32.0	36.0
38	34.60398171864054	36.0	36.0	36.0	32.0	36.0
39	34.491220721839404	36.0	36.0	36.0	32.0	36.0
40	34.5139056204431	36.0	36.0	36.0	32.0	36.0
41	34.43379679223673	36.0	36.0	36.0	32.0	36.0
42	34.398253411074826	36.0	36.0	36.0	32.0	36.0
43	34.36010423191973	36.0	36.0	36.0	32.0	36.0
44	34.2248473773366	36.0	36.0	36.0	32.0	36.0
45	34.15124007072351	36.0	36.0	36.0	32.0	36.0
46	33.96895282504828	36.0	36.0	36.0	32.0	36.0
47	33.89660949726262	36.0	36.0	36.0	32.0	36.0
48	33.79221448349229	36.0	36.0	36.0	32.0	36.0
49	33.716624101592764	36.0	36.0	36.0	27.0	36.0
50	33.35810632990218	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	1.0
20	9.0
21	18.0
22	28.0
23	92.0
24	200.0
25	445.0
26	958.0
27	1943.0
28	3247.0
29	4955.0
30	6936.0
31	9684.0
32	13266.0
33	20327.0
34	41302.0
35	166371.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.706865802246206	21.93148658319089	13.006722308944415	23.354925305618483
2	13.946005656691465	22.082596591898994	41.864706471091935	22.106691280317605
3	17.696074252269415	27.46244203674805	30.619053090817435	24.222430620165095
4	10.317180845346074	17.93923264253122	39.9246802059433	31.81890630617941
5	10.622240838006842	40.607080505443264	35.28354269913226	13.487135957417628
6	28.175607803308583	41.17012561947936	17.90624316580363	12.748023411408429
7	25.958270165280982	34.27013562752286	21.456874599214927	18.314719607981228
8	24.901865573442358	37.04051033608493	19.6691414952017	18.388482595271014
9	25.94418477072314	14.84341118602729	21.754891894596767	37.4575121486528
10	15.568067669200802	28.28050692593677	32.825270680509895	23.326154724352534
11	32.3915887954393	24.340303132517615	25.133162578813344	18.134945493229743
12	23.241642357005446	27.49246616725294	31.167642142017844	18.09824933372377
13	27.32751878361498	22.8813527909468	28.228613367039436	21.562515058398787
14	20.544659967455324	23.555598388334328	28.633383126438655	27.26635851777169
15	22.895067517226806	31.259197206643858	25.20025353710204	20.64548173902729
16	20.996367410482616	29.126325153829047	28.53213729705686	21.345170138631477
17	18.382922571103443	29.22793504409099	29.37212500417002	23.017017380635547
18	18.44940896504943	28.074623490905587	33.25845778613023	20.217509757914755
19	22.58815418317685	26.41752816152241	30.27210758276096	20.72221007253978
20	22.18078974583276	27.804939525470473	30.818101956016502	19.196168772680267
21	22.607428933624433	25.85300037437496	29.69275306449999	21.84681762750062
22	21.711894374367546	28.203037255868608	30.390721431669153	19.694346938094693
23	21.169236015612547	27.621088059662767	31.081276433281563	20.128399491443123
24	22.070041737402793	27.4010126694887	30.66883631969516	19.860109273413347
25	21.253535274908167	27.629076917944555	31.565973882519526	19.551413924627752
26	19.077625638478477	28.68908970946913	32.77535195083438	19.45793270121802
27	19.116246140387943	28.991663608630702	31.594144880477128	20.297945370504227
28	19.29170342583051	28.455670301808176	31.37218560721196	20.880440665149347
29	20.142263539685892	27.12051627060468	30.58369566426101	22.15352452544842
30	20.018311352297076	27.71612214487467	31.167107770092446	21.098458732735807
31	21.53524525448419	26.895889628995373	30.586290361441314	20.98257475507912
32	19.077254968826683	28.636825288566325	31.285259950626802	21.00065979198019
33	20.19445329933057	28.481885374116878	31.430933123781422	19.892728202771124
34	19.82793450984317	27.847402152115976	32.77992149187675	19.54474184616411
35	20.05982586004307	29.160844085802296	30.65389590893422	20.125434145220417
36	19.134708764854587	28.554536625868295	31.066564856068972	21.244189753208147
37	20.442591048095636	27.909554258178108	29.44601983134093	22.201834862385322
38	20.224031907125354	29.88142321791959	30.09085079489812	19.803694080056935
39	20.835202146942496	28.055556585204926	29.614981151238606	21.49426011661397
40	21.814731207905673	28.42861432050441	30.605935925806488	19.150718545783434
41	21.54051449329083	27.68144413966936	31.15835124916599	19.619690117873823
42	20.131150700977884	30.490869864994107	29.47443339783368	19.90354603619433
43	19.007072743987425	30.075472257643604	30.52289374573708	20.394561252631892
44	19.237726672894148	29.76587636967513	30.160283498658124	20.836113458772594
45	19.891094026852105	29.734147842267973	29.59847872663785	20.77627940424207
46	21.465824971364814	28.400544161202195	28.17591085838835	21.95772000904464
47	21.70220810367004	29.581249837830224	27.906916401943814	20.809625656555923
48	20.145530299660454	30.110982607535252	27.410924780926116	22.332562311878178
49	21.065273880439335	28.848171226707098	28.901920517772485	21.18463437508109
50	20.146564953406134	30.28601294397616	27.90368376961798	21.663738332999728
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	314.0
1	174.5
2	35.0
3	117.0
4	199.0
5	221.0
6	243.0
7	203.0
8	163.0
9	214.5
10	266.0
11	458.0
12	650.0
13	1157.5
14	1665.0
15	2370.0
16	3075.0
17	3391.5
18	3708.0
19	3622.5
20	3537.0
21	3523.5
22	3510.0
23	3195.5
24	2881.0
25	3410.5
26	3940.0
27	5583.0
28	7226.0
29	8464.5
30	9703.0
31	10495.5
32	11288.0
33	12999.5
34	14711.0
35	16722.5
36	18734.0
37	20134.5
38	21535.0
39	21084.5
40	20634.0
41	20530.5
42	20427.0
43	17937.5
44	15448.0
45	15487.0
46	15526.0
47	14804.5
48	14083.0
49	13298.0
50	12513.0
51	12695.5
52	12878.0
53	11718.0
54	10558.0
55	10157.5
56	9757.0
57	9066.5
58	8376.0
59	7391.5
60	6407.0
61	5773.5
62	5140.0
63	4205.5
64	3271.0
65	2767.5
66	2264.0
67	1870.0
68	1476.0
69	1322.5
70	1169.0
71	984.5
72	800.0
73	731.5
74	663.0
75	536.0
76	409.0
77	331.5
78	254.0
79	216.5
80	179.0
81	136.0
82	93.0
83	69.0
84	45.0
85	28.0
86	11.0
87	10.5
88	10.0
89	7.0
90	4.0
91	2.5
92	1.0
93	1.5
94	2.0
95	1.5
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0311361353384016
2	0.005189355889733601
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.001112004833514343
17	0.0
18	7.413365556762287E-4
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	3.7066827783811433E-4
25	7.413365556762287E-4
26	3.7066827783811433E-4
27	7.413365556762287E-4
28	0.003336014500543029
29	7.413365556762287E-4
30	0.0018533413891905718
31	7.413365556762287E-4
32	3.7066827783811433E-4
33	3.7066827783811433E-4
34	7.413365556762287E-4
35	0.0
36	3.7066827783811433E-4
37	0.0029653462227049146
38	0.0
39	0.0014826731113524573
40	7.413365556762287E-4
41	0.001112004833514343
42	0.006301360723247944
43	0.0055600241675717145
44	0.0040773510562192574
45	0.003336014500543029
46	0.0037066827783811436
47	0.0014826731113524573
48	0.0040773510562192574
49	0.004448019334057372
50	0.0018533413891905718
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	269783.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.002303841034745
#Duplication Level	Percentage of deduplicated	Percentage of total
1	63.15249367214515	24.63092746520029
2	17.554691586976194	13.693468302218037
3	7.211478205598246	8.437927923532285
4	3.7189810346425483	5.801953131686977
5	2.061615517424379	4.020387740698885
6	1.3875719558965198	3.2471101815104957
7	0.896834001635007	2.4485014558717717
8	0.6310672966253976	1.9690462757699325
9	0.46185352509835315	1.6212016364345043
>10	2.5690600887533943	18.646923508051305
>50	0.2289360013963589	6.219652758565928
>100	0.12243099205109627	8.458898567904189
>500	0.0029861217573438114	0.8040010525553984
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	907	0.3361961279991697	No Hit
CCATAGGGTCTTCTCGTCTTATTATTATATTTCAGCCTCTTCACTGAAAG	633	0.23463301987152635	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	531	0.1968248555320387	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	424	0.15716334980336047	No Hit
GTATTAGAGGCACTGCCTGCCCAGTGACTAAAGTTTAACGGCCGCGGTAT	404	0.1497499842465982	No Hit
ACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA	337	0.12491520963144452	No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA	334	0.1238032047979302	No Hit
CCATTGGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTAATTGTCGA	332	0.12306186824225396	No Hit
GTCAGGATACCGCGGCCGTTAAACTTTAGTCACTGGGCAGGCAGTGCCTC	311	0.11527783440765356	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	300	0.1112004833514343	No Hit
GACTATAGGCAATAATCACACTATAAATAATCCACCTATAACTTCTCTGT	298	0.11045914679575806	No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA	296	0.10971781024008184	No Hit
CTCTAATACTTGTAATGCTAGAGGTGATGTTTTTGGTAAACAGGCGGGGT	290	0.10749380057305316	No Hit
GTTCATGCTAGTCCCTAATTAAGGAACAAGTGATTATGCTACCTTTGCAC	287	0.1063817957395388	No Hit
GTCCTAGACAGTCCTTGAAAAACCGACGGAACGTATTACTCTCATGCCTG	287	0.1063817957395388	No Hit
CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA	286	0.10601112746170069	No Hit
CACTATAAATAATCCACCTATAACTTCTCTGTTAACCCAACACCGGAATG	277	0.10267511296115767	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	3.7066827783811433E-4	0.0
22	0.0	0.0	0.0	3.7066827783811433E-4	0.0
23	0.0	0.0	0.0	0.0014826731113524573	0.0
24	0.0	0.0	0.0	0.004448019334057372	0.0
25	0.0	0.0	0.0	0.006301360723247944	0.0
26	0.0	0.0	0.0	0.006672029001086058	0.0
27	0.0	0.0	0.0	0.008154702112438515	0.0
28	0.0	0.0	0.0	0.010749380057305316	0.0
29	0.0	0.0	0.0	0.013344058002172116	0.0
30	0.0	0.0	0.0	0.021869428392448747	0.0
31	0.0	0.0	0.0	0.04188551539570692	0.0
32	0.0	0.0	0.0	0.06153093412112698	0.0
33	0.0	0.0	0.0	0.07598699695681344	0.0
34	0.0	0.0	0.0	0.08970172323682367	0.0
35	0.0	0.0	0.0	0.11194181990711052	0.0
36	0.0	0.0	0.0	0.14048327730064533	0.0
37	0.0	0.0	0.0	0.18273946097419036	0.0
38	0.0	0.0	0.0	0.22944366398179278	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAAGAGG	20	7.848524E-4	43.99685	4
ACCGACG	30	1.2990033E-4	36.66404	22
CGACGGA	25	0.0023482533	35.204002	24
CGGATGC	45	1.0338317E-6	34.219772	14
CGGAACG	30	0.005734016	29.33667	27
GACGGAA	30	0.005734016	29.33667	25
CCGACGG	30	0.005734016	29.33667	23
ACGAAAG	55	4.9450737E-6	27.997993	22
TTAATCC	40	7.0132135E-4	27.50313	41
GATGCAA	125	0.0	26.39811	16
CGAAAGT	60	9.707794E-6	25.669586	23
GGATGCA	60	9.721776E-6	25.66483	15
ATACATG	95	9.513315E-10	25.481308	1
ATGCAAC	140	0.0	25.141056	17
TTTCCCC	45	0.0013959854	24.442694	10
CCGGATG	65	1.807628E-5	23.690609	13
TTTCAAT	75	2.055669E-6	23.464987	34
TGCAACG	85	2.3402208E-7	23.29245	18
GTCCTAC	365	0.0	22.910963	1
CCTACAG	360	0.0	22.609491	3
>>END_MODULE