FastQCFastQC Report
Thu 2 Feb 2017
SRR4062322_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062322_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences315988
Sequences flagged as poor quality0
Sequence length25
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCATAGGGTCTTCTCGTCTTATTAT14530.4598275883894325No Hit
GTATCAACGCAGAGTACTTTTTTTT12440.39368583617099384No Hit
TATCAACGCAGAGTACTTTTTTTTT8780.2778586528602352No Hit
GTATTAGAGGCACTGCCTGCCCAGT8390.2655164120156462No Hit
GTCAGGATACCGCGGCCGTTAAACT7820.24747775231970834No Hit
CTCTAATACTTGTAATGCTAGAGGT7180.22722381862602378No Hit
CCATTGGGATGTCCTGATCCAACAT7030.22247680291656644No Hit
GTTCATGCTAGTCCCTAATTAAGGA7000.221527399774675No Hit
CTTCTACACCATTGGGATGTCCTGA6920.2189956580629644No Hit
GGTCAGGATACCGCGGCCGTTAAAC6490.2053875463625201No Hit
ATCCTGACCGTGCAAAGGTAGCATA6470.20475461093459246No Hit
GCCTAAAGGAAAGATCCAAAAAGAT6460.20443814322062864No Hit
GACTATAGGCAATAATCACACTATA6400.20253933693684573No Hit
ATAAATAATCCACCTATAACTTCTC6330.20032406293909896No Hit
GTCCTACAGTGGACATTTCTAAATT6330.20032406293909896No Hit
GTCCTACAGTGTGCATTTCTCATTT6320.20000759522513512No Hit
ACCTATAACTTCTCTGTTAACCCAA6290.19905819208324368No Hit
CACTATAAATAATCCACCTATAACT6020.1905135638062205No Hit
GGTATCAACGCAGAGTACTTTTTTT6000.18988062837829284No Hit
GTTATATAATTTAAGCTCCATAGGG5930.1876653543805461No Hit
CCTATAACTTCTCTGTTAACCCAAC5890.18639948352469082No Hit
CTGTTAGTATGAGTAACAAGAATTC5880.18608301581072698No Hit
GATTAAACCTTGTACCTTTTGCATA5880.18608301581072698No Hit
CTTTAGGACGTGAAATATGGCGAGG5710.18070306467334205No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA5580.17658898439181234No Hit
GTCCTAAAGTGTGTATTTCTCATTT5570.17627251667784852No Hit
CTATAGAACTAGTACCGCAAGGGAA5550.17563958124992088No Hit
ATCTATAACTTTATAGATGCAACAC5510.1743737103940656No Hit
CTGTAGGACGTGGAATATGGCAAGA5490.17374077496613793No Hit
CTGTTAACCCAACACCGGAATGCCT5460.17279137182424648No Hit
GTTATAGATTAACCCAATTTTAAGT5200.16456321126118714No Hit
GATATACACTGTTCTACAAATCCCG4990.15791738926794688No Hit
GTACATGGGAGAAATCGTAAATAGA4990.15791738926794688No Hit
GTATCCTGACCGTGCAAAGGTAGCA4980.15760092155398306No Hit
GATTAAAGATAAGAGACAGTTGGAC4970.15728445384001924No Hit
CCTCTAGCATTACAAGTATTAGAGG4950.1566515184120916No Hit
GTATCAACGCAGAGTACATGGGAGA4850.15348684127245338No Hit
CTGTAGGACCTGGAATATGGCGAGA4780.15127156727470664No Hit
ATCGTAAATAGATAGAAACCGACCT4620.1462080838512855No Hit
CTATTAAAGGTTTTTTCCGTTCCAG4560.14430927756750256No Hit
TACTAACAGTGTTGCATCTATAAAG4520.14304340671164728No Hit
TTGTAGAACAGTGTATATCAATGAG4500.14241047128371964No Hit
AGCATGAACGGCTAAACGAGGGTCC4500.14241047128371964No Hit
GGATACCGCGGCCGTTAAACTTTAG4400.13924579414408142No Hit
ACCTTTGCACGGTCAGGATACCGCG4280.13544818157651556No Hit
GATATATTTTGATCAACGGACCAAG4270.13513171386255174No Hit
ACGCAGAGTACTTTTTTTTTTTTTT4250.1344987784346241No Hit
GATAGAAACCGACCTGGATTGCTCC4230.13386584300669646No Hit
GACCTGGATTGCTCCGGTCTGAACT4130.13070116586705824No Hit
TGCTAGAGGTGATGTTTTTGGTAAA4080.12911882729723914No Hit
AAATATATCTGGGTCAATAAGATAT4070.1288023595832753No Hit
GTGTATATCAATGAGTTACAATGAG4020.12722002101345622No Hit
CTTTATTGGTGGCTGCTTTTAGGCC4010.12690355329949238No Hit
GTCCTGATCCAACATCGAGGTCGTA3890.12310594073192652No Hit
GGGCAGGCAGTGCCTCTAATACTTG3760.11899186045039685No Hit
GTTAGTATGAGTAACAAGAATTCCA3710.11740952188057775No Hit
TATCAACGCAGAGTACATGGGAGAA3700.11709305416661392No Hit
GCTATCACCAAGCTCGTTAGGCTTT3670.11614365102472245No Hit
GGATTGCTCCGGTCTGAACTCAGAT3610.11424484474093953No Hit
GTTATCCCTAGGGTAACTTGGTCCG3580.11329544159904806No Hit
CATTTACACCTACTACCCAACTATC3560.11266250617112043No Hit
GTGTATATCAATGAGTTACAATGAA3490.11044723217337367No Hit
GTACTTTTTTTTTTTTTTTTTTTTT3420.10823195817562692No Hit
ATTATAACCTAGACTTACAAGTCAA3400.10759902274769928No Hit
CAGTTGGACCCTCGTTTAGCCGTTC3370.10664961960580781No Hit
GTATAGGGGTCCTAGGAAGATAATA3340.10570021646391635No Hit
AATCTAAACTTACTTTTTGATTTTG3310.10475081332202488No Hit
GTATCAACGCAGAGTACATGGGGTG3250.10285200703824195No Hit
CTATAAATAATCCACCTATAACTTC3240.10253553932427814No Hit
ACCCTATGGAGCTTAAATTATATAA3220.10190260389635049No Hit
CGTCTATGTGGCAAAATAGTGAGAA3180.10063673304049521No Hit
TCTAGGAGCTATAGAACTAGTACCG3170.10032026532653139No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCTCG250.006014699619.00380510
TGGCGAC401.2829563E-518.99477218
GGCGACA401.2829563E-518.99477219
GTTTAAA751.6916601E-1017.7340741
TTAGGCA453.5232235E-516.8895934
GTTTAGA453.5232235E-516.8895931
AGATTAT453.5232235E-516.8895935
ATTGAAC851.0077201E-915.6477128
CAATACT551.1221891E-515.5461034
TGAACTT807.616109E-915.44059210
CCGTTTC755.791844E-815.20063413
TAACTTT755.791844E-815.2006346
CCTTTAG456.739091E-414.7783941
TAGGACC2400.014.6464434
AAGATCC1300.014.61599411
ATCCCGT851.6949343E-814.53232110
TTTAAAA851.6983904E-814.5300182
GAACTTA851.6983904E-814.53001811
GTGTAAG400.005265315514.2505951
TCCAATA400.005265315514.2505952
Produced by FastQC (version 0.11.5)