##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062322_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	315988
Sequences flagged as poor quality	0
Sequence length	25
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.1637498892363	32.0	32.0	32.0	32.0	32.0
2	30.771551451320935	32.0	32.0	32.0	32.0	32.0
3	30.840054052685545	32.0	32.0	32.0	32.0	32.0
4	30.83897173310379	32.0	32.0	32.0	32.0	32.0
5	30.83808245882755	32.0	32.0	32.0	32.0	32.0
6	34.41125928832741	36.0	36.0	36.0	32.0	36.0
7	34.30587553957745	36.0	36.0	36.0	32.0	36.0
8	34.25304758408547	36.0	36.0	36.0	32.0	36.0
9	34.34398774637012	36.0	36.0	36.0	32.0	36.0
10	34.12459333898756	36.0	36.0	36.0	32.0	36.0
11	34.402923528741596	36.0	36.0	36.0	32.0	36.0
12	34.21616327202299	36.0	36.0	36.0	32.0	36.0
13	34.32361039026799	36.0	36.0	36.0	32.0	36.0
14	34.22611934630429	36.0	36.0	36.0	32.0	36.0
15	34.216172766054406	36.0	36.0	36.0	32.0	36.0
16	34.2029349215793	36.0	36.0	36.0	32.0	36.0
17	34.157654721065356	36.0	36.0	36.0	32.0	36.0
18	34.185668443105435	36.0	36.0	36.0	32.0	36.0
19	34.13159360482044	36.0	36.0	36.0	32.0	36.0
20	34.11075420585592	36.0	36.0	36.0	32.0	36.0
21	34.06622086914693	36.0	36.0	36.0	32.0	36.0
22	34.02569717837386	36.0	36.0	36.0	32.0	36.0
23	34.05142283884198	36.0	36.0	36.0	32.0	36.0
24	33.9884710811803	36.0	36.0	36.0	32.0	36.0
25	33.67943086446321	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	4.0
5	29.0
6	66.0
7	12.0
8	50.0
9	44.0
10	27.0
11	4.0
12	14.0
13	9.0
14	82.0
15	104.0
16	135.0
17	182.0
18	262.0
19	344.0
20	560.0
21	680.0
22	1043.0
23	1548.0
24	1961.0
25	2624.0
26	3589.0
27	4639.0
28	5865.0
29	7916.0
30	10385.0
31	14201.0
32	20090.0
33	29202.0
34	66192.0
35	144125.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.74650294322426	23.186910563959746	13.224571175390848	23.84201531742515
2	13.520176462955755	22.593651004629937	42.7732786475393	21.11289388487501
3	16.664029419024892	27.35880703575475	32.0726234706601	23.904540074560266
4	9.532083784348353	19.592409021566432	43.18286839223431	27.692638801850904
5	9.874698459531844	41.563831605873155	37.99409898758381	10.567370947011188
6	26.212387026492962	43.94012324958357	19.12110253275994	10.726387191163523
7	25.174554900842676	35.276246594437055	22.315466007729835	17.233732496990434
8	22.679419470731172	39.302313056205904	20.617596461825542	17.400671011237378
9	24.500562411875602	16.857781087118394	23.086453002962564	35.55520349804344
10	14.185983870201069	28.4727393722371	35.772661733715715	21.568615023846117
11	30.331734029674795	25.64093625813648	27.55756542461829	16.46976428757043
12	21.52566970651423	29.46074920940726	31.62339267267876	17.390188411399745
13	25.01346649852027	23.461492151407803	29.4342803186332	22.09076103143873
14	18.700237939086325	26.566801952938118	29.041875378215426	25.691084729760128
15	21.818792541797116	33.54036479789082	24.754731091478334	19.886111568833737
16	20.59413406670701	30.3955085086766	29.02581910468001	19.98453831993638
17	17.420937063335902	31.86534982240346	29.599211667823184	21.114501446437455
18	17.361786518740516	30.913059593921172	32.51038323728905	19.21477065004926
19	21.046929692573293	29.838288167001913	29.409062831620126	19.70571930880466
20	19.846312708659323	31.898031713049484	30.073549441569053	18.18210613672214
21	21.19612041659276	28.61857942883207	28.681296404226742	21.504003750348428
22	20.230616931971173	31.28439059158945	29.160053852854993	19.324938623584384
23	20.516767384997404	31.038032255203778	29.625441830413134	18.819758529385684
24	20.656002534051314	31.17896737408933	29.479885967690844	18.685144124168517
25	20.177314062544543	30.72240118336073	31.148423716626915	17.95186103746781
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	649.0
1	649.0
2	444.0
3	239.0
4	239.0
5	239.0
6	740.0
7	1241.0
8	1241.0
9	1241.0
10	1283.0
11	1325.0
12	1325.0
13	1325.0
14	1911.0
15	2497.0
16	2497.0
17	2497.0
18	4602.0
19	6707.0
20	6707.0
21	6707.0
22	9794.0
23	12881.0
24	12881.0
25	12881.0
26	19939.5
27	26998.0
28	26998.0
29	26998.0
30	32823.0
31	38648.0
32	38648.0
33	38648.0
34	40421.0
35	42194.0
36	42194.0
37	42194.0
38	41156.0
39	40118.0
40	40118.0
41	40118.0
42	37416.0
43	34714.0
44	34714.0
45	34714.0
46	37116.5
47	39519.0
48	39519.0
49	39519.0
50	34399.0
51	29279.0
52	29279.0
53	29279.0
54	23671.0
55	18063.0
56	18063.0
57	18063.0
58	13930.0
59	9797.0
60	9797.0
61	9797.0
62	7421.5
63	5046.0
64	5046.0
65	5046.0
66	3987.5
67	2929.0
68	2929.0
69	2929.0
70	2280.0
71	1631.0
72	1631.0
73	1631.0
74	1250.5
75	870.0
76	870.0
77	870.0
78	602.5
79	335.0
80	335.0
81	335.0
82	225.5
83	116.0
84	116.0
85	116.0
86	81.0
87	46.0
88	46.0
89	46.0
90	31.0
91	16.0
92	16.0
93	16.0
94	17.5
95	19.0
96	19.0
97	19.0
98	65.0
99	111.0
100	111.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0025317417117105712
2	3.164677139638214E-4
3	6.329354279276428E-4
4	0.010759902274769929
5	0.034811448536020355
6	0.06519234907654721
7	0.10380141018013342
8	0.10918136131751839
9	0.12120713444814361
10	0.13386584300669646
11	0.13734698786029848
12	0.12722002101345622
13	0.12342240844589035
14	0.11424484474093953
15	0.1316505690089497
16	0.1167765864526501
17	0.12184006987607124
18	0.10601668417788018
19	0.09588971733103789
20	0.08924389533779764
21	0.08987683076572528
22	0.0990543944706761
23	0.08101573477473828
24	0.09114270162158056
25	0.08766155676797853
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	315988.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.905173614187877
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.92134003198113	18.95356785700723
2	14.23954092337004	7.9471372330594825
3	5.621647368361364	4.706191374355988
4	3.004184764734568	3.353291897160652
5	1.7532916746997516	2.4462954289403394
6	1.06490354627624	1.78297910047217
7	0.8256121210746566	1.612719470359634
8	0.6135386778865237	1.369672266035419
9	0.4627056942286537	1.1620694456751524
>10	3.613187112285517	20.68622859095915
>50	0.48311918073874144	9.479473904072307
>100	0.36177234426210914	19.827651682975304
>500	0.032888394932919014	5.819208324366748
>1k	0.002268165167787518	0.8535134245604264
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCATAGGGTCTTCTCGTCTTATTAT	1453	0.4598275883894325	No Hit
GTATCAACGCAGAGTACTTTTTTTT	1244	0.39368583617099384	No Hit
TATCAACGCAGAGTACTTTTTTTTT	878	0.2778586528602352	No Hit
GTATTAGAGGCACTGCCTGCCCAGT	839	0.2655164120156462	No Hit
GTCAGGATACCGCGGCCGTTAAACT	782	0.24747775231970834	No Hit
CTCTAATACTTGTAATGCTAGAGGT	718	0.22722381862602378	No Hit
CCATTGGGATGTCCTGATCCAACAT	703	0.22247680291656644	No Hit
GTTCATGCTAGTCCCTAATTAAGGA	700	0.221527399774675	No Hit
CTTCTACACCATTGGGATGTCCTGA	692	0.2189956580629644	No Hit
GGTCAGGATACCGCGGCCGTTAAAC	649	0.2053875463625201	No Hit
ATCCTGACCGTGCAAAGGTAGCATA	647	0.20475461093459246	No Hit
GCCTAAAGGAAAGATCCAAAAAGAT	646	0.20443814322062864	No Hit
GACTATAGGCAATAATCACACTATA	640	0.20253933693684573	No Hit
ATAAATAATCCACCTATAACTTCTC	633	0.20032406293909896	No Hit
GTCCTACAGTGGACATTTCTAAATT	633	0.20032406293909896	No Hit
GTCCTACAGTGTGCATTTCTCATTT	632	0.20000759522513512	No Hit
ACCTATAACTTCTCTGTTAACCCAA	629	0.19905819208324368	No Hit
CACTATAAATAATCCACCTATAACT	602	0.1905135638062205	No Hit
GGTATCAACGCAGAGTACTTTTTTT	600	0.18988062837829284	No Hit
GTTATATAATTTAAGCTCCATAGGG	593	0.1876653543805461	No Hit
CCTATAACTTCTCTGTTAACCCAAC	589	0.18639948352469082	No Hit
CTGTTAGTATGAGTAACAAGAATTC	588	0.18608301581072698	No Hit
GATTAAACCTTGTACCTTTTGCATA	588	0.18608301581072698	No Hit
CTTTAGGACGTGAAATATGGCGAGG	571	0.18070306467334205	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	558	0.17658898439181234	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	557	0.17627251667784852	No Hit
CTATAGAACTAGTACCGCAAGGGAA	555	0.17563958124992088	No Hit
ATCTATAACTTTATAGATGCAACAC	551	0.1743737103940656	No Hit
CTGTAGGACGTGGAATATGGCAAGA	549	0.17374077496613793	No Hit
CTGTTAACCCAACACCGGAATGCCT	546	0.17279137182424648	No Hit
GTTATAGATTAACCCAATTTTAAGT	520	0.16456321126118714	No Hit
GATATACACTGTTCTACAAATCCCG	499	0.15791738926794688	No Hit
GTACATGGGAGAAATCGTAAATAGA	499	0.15791738926794688	No Hit
GTATCCTGACCGTGCAAAGGTAGCA	498	0.15760092155398306	No Hit
GATTAAAGATAAGAGACAGTTGGAC	497	0.15728445384001924	No Hit
CCTCTAGCATTACAAGTATTAGAGG	495	0.1566515184120916	No Hit
GTATCAACGCAGAGTACATGGGAGA	485	0.15348684127245338	No Hit
CTGTAGGACCTGGAATATGGCGAGA	478	0.15127156727470664	No Hit
ATCGTAAATAGATAGAAACCGACCT	462	0.1462080838512855	No Hit
CTATTAAAGGTTTTTTCCGTTCCAG	456	0.14430927756750256	No Hit
TACTAACAGTGTTGCATCTATAAAG	452	0.14304340671164728	No Hit
TTGTAGAACAGTGTATATCAATGAG	450	0.14241047128371964	No Hit
AGCATGAACGGCTAAACGAGGGTCC	450	0.14241047128371964	No Hit
GGATACCGCGGCCGTTAAACTTTAG	440	0.13924579414408142	No Hit
ACCTTTGCACGGTCAGGATACCGCG	428	0.13544818157651556	No Hit
GATATATTTTGATCAACGGACCAAG	427	0.13513171386255174	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	425	0.1344987784346241	No Hit
GATAGAAACCGACCTGGATTGCTCC	423	0.13386584300669646	No Hit
GACCTGGATTGCTCCGGTCTGAACT	413	0.13070116586705824	No Hit
TGCTAGAGGTGATGTTTTTGGTAAA	408	0.12911882729723914	No Hit
AAATATATCTGGGTCAATAAGATAT	407	0.1288023595832753	No Hit
GTGTATATCAATGAGTTACAATGAG	402	0.12722002101345622	No Hit
CTTTATTGGTGGCTGCTTTTAGGCC	401	0.12690355329949238	No Hit
GTCCTGATCCAACATCGAGGTCGTA	389	0.12310594073192652	No Hit
GGGCAGGCAGTGCCTCTAATACTTG	376	0.11899186045039685	No Hit
GTTAGTATGAGTAACAAGAATTCCA	371	0.11740952188057775	No Hit
TATCAACGCAGAGTACATGGGAGAA	370	0.11709305416661392	No Hit
GCTATCACCAAGCTCGTTAGGCTTT	367	0.11614365102472245	No Hit
GGATTGCTCCGGTCTGAACTCAGAT	361	0.11424484474093953	No Hit
GTTATCCCTAGGGTAACTTGGTCCG	358	0.11329544159904806	No Hit
CATTTACACCTACTACCCAACTATC	356	0.11266250617112043	No Hit
GTGTATATCAATGAGTTACAATGAA	349	0.11044723217337367	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	342	0.10823195817562692	No Hit
ATTATAACCTAGACTTACAAGTCAA	340	0.10759902274769928	No Hit
CAGTTGGACCCTCGTTTAGCCGTTC	337	0.10664961960580781	No Hit
GTATAGGGGTCCTAGGAAGATAATA	334	0.10570021646391635	No Hit
AATCTAAACTTACTTTTTGATTTTG	331	0.10475081332202488	No Hit
GTATCAACGCAGAGTACATGGGGTG	325	0.10285200703824195	No Hit
CTATAAATAATCCACCTATAACTTC	324	0.10253553932427814	No Hit
ACCCTATGGAGCTTAAATTATATAA	322	0.10190260389635049	No Hit
CGTCTATGTGGCAAAATAGTGAGAA	318	0.10063673304049521	No Hit
TCTAGGAGCTATAGAACTAGTACCG	317	0.10032026532653139	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGCTCG	25	0.0060146996	19.003805	10
TGGCGAC	40	1.2829563E-5	18.994772	18
GGCGACA	40	1.2829563E-5	18.994772	19
GTTTAAA	75	1.6916601E-10	17.734074	1
TTAGGCA	45	3.5232235E-5	16.889593	4
GTTTAGA	45	3.5232235E-5	16.889593	1
AGATTAT	45	3.5232235E-5	16.889593	5
ATTGAAC	85	1.0077201E-9	15.647712	8
CAATACT	55	1.1221891E-5	15.546103	4
TGAACTT	80	7.616109E-9	15.440592	10
CCGTTTC	75	5.791844E-8	15.200634	13
TAACTTT	75	5.791844E-8	15.200634	6
CCTTTAG	45	6.739091E-4	14.778394	1
TAGGACC	240	0.0	14.646443	4
AAGATCC	130	0.0	14.615994	11
ATCCCGT	85	1.6949343E-8	14.532321	10
TTTAAAA	85	1.6983904E-8	14.530018	2
GAACTTA	85	1.6983904E-8	14.530018	11
GTGTAAG	40	0.0052653155	14.250595	1
TCCAATA	40	0.0052653155	14.250595	2
>>END_MODULE