FastQCFastQC Report
Thu 2 Feb 2017
SRR4062322_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062322_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences315988
Sequences flagged as poor quality0
Sequence length50
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCATAGGGTCTTCTCGTCTTATTATTATATTTCAGCCTCTTCACTGAAAG13380.423433801283593No Hit
GTATTAGAGGCACTGCCTGCCCAGTGACTAAAGTTTAACGGCCGCGGTAT8650.27374457257870555No Hit
GTCAGGATACCGCGGCCGTTAAACTTTAGTCACTGGGCAGGCAGTGCCTC7180.22722381862602378No Hit
CCATTGGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTAATTGTCGA7030.22247680291656644No Hit
CCTATAACTTCTCTGTTAACCCAACACCGGAATGCCTAAAGGAAAGATCC6890.21804625492107296No Hit
ATCCTGACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGACT6780.21456511006747092No Hit
ACCTATAACTTCTCTGTTAACCCAACACCGGAATGCCTAAAGGAAAGATC6730.2129827714976518No Hit
CACTATAAATAATCCACCTATAACTTCTCTGTTAACCCAACACCGGAATG6550.20728635264630302No Hit
CTTCTACACCATTGGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTA6530.20665341721837538No Hit
ATAAATAATCCACCTATAACTTCTCTGTTAACCCAACACCGGAATGCCTA6530.20665341721837538No Hit
CTCTAATACTTGTAATGCTAGAGGTGATGTTTTTGGTAAACAGGCGGGGT6530.20665341721837538No Hit
CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA6250.19779232122738838No Hit
GACTATAGGCAATAATCACACTATAAATAATCCACCTATAACTTCTCTGT6210.1965264503715331No Hit
GCCTAAAGGAAAGATCCAAAAAGATAAAAGGAACTCGGCAAACAAGAACC6070.1920959023760396No Hit
GTTCATGCTAGTCCCTAATTAAGGAACAAGTGATTATGCTACCTTTGCAC5990.18956416066432902No Hit
ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC5990.18956416066432902No Hit
GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC5900.18671595123865464No Hit
CTGTTAACCCAACACCGGAATGCCTAAAGGAAAGATCCAAAAAGATAAAA5820.18418420952694406No Hit
GTTATATAATTTAAGCTCCATAGGGTCTTCTCGTCTTATTATTATATTTC5700.1803865969593782No Hit
GGTCAGGATACCGCGGCCGTTAAACTTTAGTCACTGGGCAGGCAGTGCCT5570.17627251667784852No Hit
CTATAGAACTAGTACCGCAAGGGAAAGATGAAAGACTAATTAAAAGTAAG5240.16582908211704242No Hit
GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA5160.16329734040533184No Hit
GTATCCTGACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGA5130.16234793726344038No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4990.15791738926794688No Hit
ATCGTAAATAGATAGAAACCGACCTGGATTGCTCCGGTCTGAACTCAGAT4860.15380330898641723No Hit
CCTCTAGCATTACAAGTATTAGAGGCACTGCCTGCCCAGTGACTAAAGTT4790.15158803498867046No Hit
GTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAACA4790.15158803498867046No Hit
GTATCAACGCAGAGTACATGGGAGAAATCGTAAATAGATAGAAACCGACC4720.1493727609909237No Hit
GTACATGGGAGAAATCGTAAATAGATAGAAACCGACCTGGATTGCTCCGG4600.14557514842335784No Hit
TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT4570.14462574528146638No Hit
GATTAAAGATAAGAGACAGTTGGACCCTCGTTTAGCCGTTCATGCTAGTC4560.14430927756750256No Hit
ACCTTTGCACGGTCAGGATACCGCGGCCGTTAAACTTTAGTCACTGGGCA4310.13639758471840702No Hit
CTTTATTGGTGGCTGCTTTTAGGCCTACAATGGTTAAAAGCTGTTTTGTT4280.13544818157651556No Hit
TGCTAGAGGTGATGTTTTTGGTAAACAGGCGGGGTTCTTGTTTGCCGAGT4240.13418231072066028No Hit
GATAGAAACCGACCTGGATTGCTCCGGTCTGAACTCAGATCACGTAGGAC4130.13070116586705824No Hit
GATATATTTTGATCAACGGACCAAGTTACCCTAGGGATAACAGCGCAATC4110.1300682304391306No Hit
GACCTGGATTGCTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCGTT4020.12722002101345622No Hit
CTATTAAAGGTTTTTTCCGTTCCAGAAGAGCTGTCCCTCTTTTGGCTATA4020.12722002101345622No Hit
CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCA3980.12595415015760092No Hit
AGCATGAACGGCTAAACGAGGGTCCAACTGTCTCTTATCTTTAATCAGTG3970.1256376824436371No Hit
GTCCTGATCCAACATCGAGGTCGTAAACCCTAATTGTCGATATGAACTCT3960.12532121472967328No Hit
GGATACCGCGGCCGTTAAACTTTAGTCACTGGGCAGGCAGTGCCTCTAAT3940.12468827930174563No Hit
AAATATATCTGGGTCAATAAGATATGTTGATTTTACTTTGACTTGTAAGT3850.12184006987607124No Hit
GATATACACTGTTCTACAAATCCCGTTTCCAACGAATGTGTTTTTCAGTG3790.11994126359228831No Hit
GCTATCACCAAGCTCGTTAGGCTTTTCACCTCTACCTAAAAATCTTCTCA3720.11772598959454157No Hit
GTTATCCCTAGGGTAACTTGGTCCGTTGATCAAAATATATCTGGGTCAAT3710.11740952188057775No Hit
CTATAAATAATCCACCTATAACTTCTCTGTTAACCCAACACCGGAATGCC3510.11108016760130132No Hit
GGATTGCTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCGTTGAACA3510.11108016760130132No Hit
CTTAAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGGTCCGTTGATCA3400.10759902274769928No Hit
CAGTTGGACCCTCGTTTAGCCGTTCATGCTAGTCCCTAATTAAGGAACAA3350.10601668417788018No Hit
GTATAGGGGTCCTAGGAAGATAATAGTTGTTAGGGCGTTTATTAGAATAA3310.10475081332202488No Hit
GTTATACGCGTATGCCTGGAGAATTGGAATTCTTGTTACTCATACTAACA3290.10411787789409725No Hit
AATCTAAACTTACTTTTTGATTTTGTTGTTTTTTTAGCAAGTTTAAAATT3290.10411787789409725No Hit
TCTAGGAGCTATAGAACTAGTACCGCAAGGGAAAGATGAAAGACTAATTA3270.1034849424661696No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3260.10316847475220578No Hit
ATTTCAGCCTCTTCACTGAAAGGTCAATTTCACTGATTAAAGATAAGAGA3240.10253553932427814No Hit
ACCCTATGGAGCTTAAATTATATAACTTATCTATTTAATTTATTAAACCT3230.10221907161031432No Hit
CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT3220.10190260389635049No Hit
GGGCAGGCAGTGCCTCTAATACTTGTAATGCTAGAGGTGATGTTTTTGGT3210.10158613618238667No Hit
ATTATAACCTAGACTTACAAGTCAAAGTAAAATCAACATATCTTATTGAC3210.10158613618238667No Hit
ATGCAACACTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATAC3180.10063673304049521No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTTCGC301.2995103E-436.664038
AACAGTA301.2995103E-436.6640314
GCGAACA250.00235100135.19746811
CTGATAT401.8167359E-532.9976234
GTTCGCA353.2138822E-431.4263089
GACTGCG353.2138822E-431.4263087
GTATAGA502.3525845E-630.802661
AGGGCTC454.053375E-529.3405141
GGGCGTT300.005736254629.33586533
TAGAGTG602.9355942E-729.3312235
TGTGATA300.005740694229.33122318
TGCGAAC300.005740694229.33122310
TATACTG300.005740694229.3312235
AGCACTA300.005740694229.33122324
TTAGGAC2150.028.6491013
GACGTGA2000.028.5979447
TAGGACG3850.028.5693744
AGATTAT703.5755875E-828.2836785
TAGGACC2450.027.8347324
CTGTAGG4350.027.8184931
Produced by FastQC (version 0.11.5)