##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062321_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10888
Sequences flagged as poor quality	0
Sequence length	25
%GC	58
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.40356355620867	32.0	32.0	32.0	32.0	32.0
2	31.16421748714181	32.0	32.0	32.0	32.0	32.0
3	31.17413666421749	32.0	32.0	32.0	32.0	32.0
4	31.184698750918443	32.0	32.0	32.0	32.0	32.0
5	31.166421748714182	32.0	32.0	32.0	32.0	32.0
6	34.751377663482735	36.0	36.0	36.0	32.0	36.0
7	34.66302351212344	36.0	36.0	36.0	32.0	36.0
8	34.75303085966201	36.0	36.0	36.0	32.0	36.0
9	34.82779206465834	36.0	36.0	36.0	32.0	36.0
10	34.5134092578986	36.0	36.0	36.0	32.0	36.0
11	34.68332108743571	36.0	36.0	36.0	32.0	36.0
12	34.65310433504776	36.0	36.0	36.0	32.0	36.0
13	34.61168258633358	36.0	36.0	36.0	32.0	36.0
14	34.64796105804555	36.0	36.0	36.0	32.0	36.0
15	34.589639970609845	36.0	36.0	36.0	32.0	36.0
16	34.61342762674504	36.0	36.0	36.0	32.0	36.0
17	34.60580455547392	36.0	36.0	36.0	32.0	36.0
18	34.54307494489346	36.0	36.0	36.0	32.0	36.0
19	34.53324761204996	36.0	36.0	36.0	32.0	36.0
20	34.508817046289494	36.0	36.0	36.0	32.0	36.0
21	34.54013592946363	36.0	36.0	36.0	32.0	36.0
22	34.359478324761206	36.0	36.0	36.0	32.0	36.0
23	34.41568699485672	36.0	36.0	36.0	32.0	36.0
24	34.366642174871416	36.0	36.0	36.0	32.0	36.0
25	34.032604702424685	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	2.0
7	0.0
8	3.0
9	3.0
10	1.0
11	0.0
12	0.0
13	1.0
14	2.0
15	1.0
16	5.0
17	8.0
18	9.0
19	16.0
20	19.0
21	18.0
22	16.0
23	26.0
24	33.0
25	59.0
26	84.0
27	108.0
28	140.0
29	190.0
30	283.0
31	385.0
32	610.0
33	883.0
34	2260.0
35	5723.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.583394562821454	16.95444526083762	9.992652461425422	26.4695077149155
2	21.886480529022776	16.357457751653197	31.520940484937547	30.23512123438648
3	22.96105804555474	18.938280675973548	25.606171932402646	32.49448934606907
4	16.69881510057867	15.091393404978415	29.70515293469275	38.50463855975016
5	19.807073954983924	30.335323840146994	30.124023886081762	19.733578318787323
6	38.09611320407976	30.129559864008087	15.179637967472203	16.594688964439953
7	33.18315557190144	26.1125413755057	17.66274365575579	23.041559396837073
8	29.195402298850574	28.606896551724137	16.855172413793102	25.342528735632186
9	26.864710751402555	12.397682332382967	17.207762347098317	43.52984456911616
10	21.40557446417073	20.81685217551283	25.029896053720908	32.74767730659553
11	42.732290708371664	18.031278748850045	18.813247470101196	20.42318307267709
12	25.834943417057687	19.49581378231668	24.574477872849386	30.094764927776247
13	34.61503081593229	15.481556434550638	21.40557446417073	28.497838285346333
14	26.588797939851005	15.469511634323554	20.206014899291823	37.73567552653361
15	31.35234590616375	21.996320147194112	17.083716651333948	29.567617295308185
16	30.675128771155265	21.716335540838852	19.66519499632082	27.943340691685066
17	28.853936718175127	21.422001471670345	20.962104488594555	28.761957321559972
18	29.5318679297342	21.585578957049574	20.32557711763083	28.556975995585393
19	29.26896551724138	21.213793103448275	20.45057471264368	29.06666666666667
20	28.595071717543213	20.56822361162192	20.761309304891505	30.075395365943365
21	29.38689217758985	20.48901553451604	20.783160216931705	29.340932070962406
22	29.869413279382012	21.243332720250137	20.498436637851754	28.388817362516093
23	29.481522338665194	20.417356131641846	20.711527854384997	29.389593675307964
24	29.698418536226555	20.18205222508275	20.95439499816109	29.165134240529607
25	29.006159786705894	20.759400570010115	20.80536912751678	29.429070515767215
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	1.0
3	2.0
4	2.0
5	2.0
6	5.0
7	8.0
8	8.0
9	8.0
10	7.0
11	6.0
12	6.0
13	6.0
14	12.0
15	18.0
16	18.0
17	18.0
18	30.5
19	43.0
20	43.0
21	43.0
22	63.0
23	83.0
24	83.0
25	83.0
26	130.0
27	177.0
28	177.0
29	177.0
30	201.5
31	226.0
32	226.0
33	226.0
34	317.0
35	408.0
36	408.0
37	408.0
38	456.0
39	504.0
40	504.0
41	504.0
42	555.5
43	607.0
44	607.0
45	607.0
46	644.0
47	681.0
48	681.0
49	681.0
50	802.0
51	923.0
52	923.0
53	923.0
54	1025.0
55	1127.0
56	1127.0
57	1127.0
58	1214.5
59	1302.0
60	1302.0
61	1302.0
62	1364.5
63	1427.0
64	1427.0
65	1427.0
66	1313.5
67	1200.0
68	1200.0
69	1200.0
70	1111.5
71	1023.0
72	1023.0
73	1023.0
74	830.0
75	637.0
76	637.0
77	637.0
78	466.0
79	295.0
80	295.0
81	295.0
82	212.0
83	129.0
84	129.0
85	129.0
86	83.0
87	37.0
88	37.0
89	37.0
90	24.5
91	12.0
92	12.0
93	12.0
94	8.0
95	4.0
96	4.0
97	4.0
98	6.5
99	9.0
100	9.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.009184423218221894
5	0.027553269654665688
6	0.04592211609110948
7	0.11021307861866275
8	0.11939750183688465
9	0.13776634827332845
10	0.15613519470977222
11	0.16531961792799413
12	0.174504041146216
13	0.15613519470977222
14	0.13776634827332845
15	0.16531961792799413
16	0.1469507714915503
17	0.1469507714915503
18	0.13776634827332845
19	0.11939750183688465
20	0.11021307861866275
21	0.08265980896399706
22	0.12858192505510654
23	0.09184423218221896
24	0.11021307861866275
25	0.10102865540044086
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	10888.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	92.00036737692872
#Duplication Level	Percentage of deduplicated	Percentage of total
1	92.13337326544874	84.76304188096988
2	7.117899570729759	13.096987509184425
3	0.6888289907157832	1.9011756061719325
4	0.0499151442547669	0.18368846436443792
5	0.0	0.0
6	0.009983028850953379	0.055106539309331376
7	0.0	0.0
8	0.0	0.0
9	0.0	0.0
>10	0.0	0.0
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE