##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062321_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10888
Sequences flagged as poor quality	0
Sequence length	50
%GC	58
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.338445995591478	32.0	32.0	32.0	32.0	32.0
2	31.46730345334313	32.0	32.0	32.0	32.0	32.0
3	31.50955180014695	32.0	32.0	32.0	32.0	32.0
4	31.628857457751653	32.0	32.0	32.0	32.0	32.0
5	31.58614988978692	32.0	32.0	32.0	32.0	32.0
6	35.16265613519471	36.0	36.0	36.0	36.0	36.0
7	35.18506612784717	36.0	36.0	36.0	36.0	36.0
8	35.19654665686995	36.0	36.0	36.0	36.0	36.0
9	35.22997795738428	36.0	36.0	36.0	36.0	36.0
10	35.035268185157975	36.0	36.0	36.0	36.0	36.0
11	35.19792432035268	36.0	36.0	36.0	36.0	36.0
12	35.17184055841293	36.0	36.0	36.0	36.0	36.0
13	35.14842027920647	36.0	36.0	36.0	36.0	36.0
14	35.06107641440118	36.0	36.0	36.0	36.0	36.0
15	35.066495224099924	36.0	36.0	36.0	36.0	36.0
16	35.10405951506245	36.0	36.0	36.0	36.0	36.0
17	35.11627479794269	36.0	36.0	36.0	36.0	36.0
18	35.039033798677444	36.0	36.0	36.0	36.0	36.0
19	35.048769287288756	36.0	36.0	36.0	36.0	36.0
20	35.08431300514328	36.0	36.0	36.0	36.0	36.0
21	35.03361498897869	36.0	36.0	36.0	36.0	36.0
22	35.0	36.0	36.0	36.0	36.0	36.0
23	34.977865540044085	36.0	36.0	36.0	32.0	36.0
24	34.95931300514328	36.0	36.0	36.0	32.0	36.0
25	34.893919911829535	36.0	36.0	36.0	32.0	36.0
26	34.842946362968405	36.0	36.0	36.0	32.0	36.0
27	34.842854518736225	36.0	36.0	36.0	32.0	36.0
28	34.897042615723734	36.0	36.0	36.0	32.0	36.0
29	34.85066127847171	36.0	36.0	36.0	32.0	36.0
30	34.88868479059515	36.0	36.0	36.0	32.0	36.0
31	34.822097722263045	36.0	36.0	36.0	32.0	36.0
32	34.859845701689935	36.0	36.0	36.0	32.0	36.0
33	34.77075679647318	36.0	36.0	36.0	32.0	36.0
34	34.73098824393828	36.0	36.0	36.0	32.0	36.0
35	34.78389052167524	36.0	36.0	36.0	32.0	36.0
36	34.70885378398236	36.0	36.0	36.0	32.0	36.0
37	34.68662747979427	36.0	36.0	36.0	32.0	36.0
38	34.62408155767818	36.0	36.0	36.0	32.0	36.0
39	34.626377663482735	36.0	36.0	36.0	32.0	36.0
40	34.64456282145481	36.0	36.0	36.0	32.0	36.0
41	34.61535635562087	36.0	36.0	36.0	32.0	36.0
42	34.55960690668626	36.0	36.0	36.0	32.0	36.0
43	34.629684055841295	36.0	36.0	36.0	32.0	36.0
44	34.60516164584864	36.0	36.0	36.0	32.0	36.0
45	34.56447465099192	36.0	36.0	36.0	32.0	36.0
46	34.521675238795005	36.0	36.0	36.0	32.0	36.0
47	34.541880969875095	36.0	36.0	36.0	32.0	36.0
48	34.51644011756062	36.0	36.0	36.0	32.0	36.0
49	34.48365172667157	36.0	36.0	36.0	32.0	36.0
50	34.005602498163114	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
21	1.0
22	3.0
23	3.0
24	9.0
25	18.0
26	44.0
27	51.0
28	115.0
29	153.0
30	258.0
31	304.0
32	464.0
33	844.0
34	1962.0
35	6659.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.57729402039129	18.214384127858914	9.488380637457517	26.719941214292277
2	20.94048493754592	17.413666421748715	31.69544452608376	29.950404114621605
3	22.299779573842763	19.709772226304185	25.5878030859662	32.40264511388685
4	16.17376928728876	15.0165319617928	30.19838354151359	38.61131520940485
5	19.47097722263042	30.602498163115357	29.66568699485672	20.260837619397503
6	38.27149155033064	29.720793534166056	14.805290227773696	17.20242468772961
7	32.42101396032329	25.09184423218222	18.708670095518002	23.778471711976486
8	30.216752387950034	29.20646583394563	16.789125642909625	23.78765613519471
9	26.680749448934606	12.196914033798677	17.551432770022043	43.57090374724467
10	21.041513592946362	20.19654665686995	25.431667891256428	33.330271858927254
11	43.02902277736958	17.845334313005143	19.443423952975753	19.68221895664952
12	25.284717119764878	19.42505510653931	24.7060984570169	30.584129316678915
13	35.35084496693608	15.301249081557678	21.179279941219693	28.16862601028655
14	24.843864805290227	15.41146216017634	20.260837619397503	39.48383541513593
15	31.78728875826598	20.995591476855253	17.578986039676707	29.63813372520206
16	30.069801616458484	22.58449669360764	19.19544452608376	28.150257163850114
17	28.471711976487878	21.987509184423217	21.65686994856723	27.883908890521674
18	28.572740631888315	21.36296840558413	21.40889052167524	28.655400440852315
19	29.711609110947833	21.50991917707568	20.52718589272594	28.251285819250548
20	30.253490080822925	20.472079353416607	20.472079353416607	28.80235121234386
21	28.903379867744306	21.592578986039676	20.178177810433503	29.325863335782515
22	29.693240264511388	21.21601763409258	20.141440117560617	28.94930198383541
23	29.24320352681852	20.995591476855253	20.609845701689935	29.151359294636297
24	29.45444526083762	20.67413666421749	20.554739162380603	29.316678912564292
25	29.904481998530493	20.29757531227039	20.619030124908157	29.178912564290965
26	28.462527553269656	21.17009551800147	20.912931667891257	29.45444526083762
27	29.022777369581192	20.426157237325494	20.224099926524612	30.3269654665687
28	28.995224099926524	21.500734753857458	20.306759735488612	29.197281410727406
29	29.45444526083762	20.710874357090375	20.73842762674504	29.09625275532697
30	30.299412196914034	20.44452608376194	20.67413666421749	28.581925055106538
31	29.20646583394563	20.79353416605437	20.720058780308598	29.279941219691402
32	28.88501102130786	21.0139603232917	20.87619397501837	29.224834680382074
33	28.940117560617196	21.151726671565026	20.407788390889053	29.500367376928725
34	29.380969875091843	20.54555473916238	20.79353416605437	29.279941219691402
35	29.436076414401175	20.536370315944158	20.87619397501837	29.151359294636297
36	29.151359294636297	20.664952240999266	20.857825128581926	29.325863335782515
37	29.684055841293166	20.095518001469507	20.554739162380603	29.66568699485672
38	29.821822189566493	20.178177810433503	20.453710506980162	29.546289493019838
39	28.930933137398974	21.206833210874358	20.894562821454812	28.967670830271857
40	28.783982365907423	20.655767817781044	21.344599559147685	29.21565025716385
41	29.49118295371051	21.032329169728143	21.317046289493017	28.159441587068336
42	28.91256429096253	21.206833210874358	20.563923585598825	29.316678912564292
43	28.97685525349008	20.977222630418808	20.775165319617926	29.27075679647318
44	29.169728141072742	20.775165319617926	20.903747244673035	29.151359294636297
45	29.546289493019838	20.848640705363703	20.68332108743571	28.92174871418075
46	28.9860396767083	21.289493019838353	20.086333578251285	29.63813372520206
47	29.429594929732712	20.94240837696335	21.640488656195462	27.987508037108476
48	28.575365114356572	20.85974097547534	19.9871406264352	30.577753283732896
49	29.188096987509184	20.995591476855253	20.49963262307127	29.316678912564292
50	29.301001194084687	21.199595848259392	20.80462937448333	28.69477358317259
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.5
10	1.0
11	0.5
12	0.0
13	1.5
14	3.0
15	2.5
16	2.0
17	3.0
18	4.0
19	5.5
20	7.0
21	15.5
22	24.0
23	20.0
24	16.0
25	28.5
26	41.0
27	55.0
28	69.0
29	81.0
30	93.0
31	111.5
32	130.0
33	144.5
34	159.0
35	171.0
36	183.0
37	198.0
38	213.0
39	245.5
40	278.0
41	285.0
42	292.0
43	298.0
44	304.0
45	308.0
46	312.0
47	318.0
48	324.0
49	327.0
50	330.0
51	365.5
52	401.0
53	433.0
54	465.0
55	497.0
56	529.0
57	613.5
58	698.0
59	720.5
60	743.0
61	771.0
62	799.0
63	844.0
64	889.0
65	839.5
66	790.0
67	778.5
68	767.0
69	677.5
70	588.0
71	544.0
72	500.0
73	437.0
74	374.0
75	317.0
76	260.0
77	205.5
78	151.0
79	115.5
80	80.0
81	57.5
82	35.0
83	25.5
84	16.0
85	10.5
86	5.0
87	4.5
88	4.0
89	3.0
90	2.0
91	1.5
92	1.0
93	0.5
94	0.0
95	1.0
96	2.0
97	3.0
98	4.0
99	2.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009184423218221894
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.009184423218221894
48	0.009184423218221894
49	0.0
50	0.009184423218221894
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	10888.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	92.01873622336517
#Duplication Level	Percentage of deduplicated	Percentage of total
1	92.13494360714643	84.78141072740631
2	7.106497654456533	13.07861866274798
3	0.7086535582393453	1.9562821454812636
4	0.04990518015770037	0.18368846436443792
5	0.0	0.0
6	0.0	0.0
7	0.0	0.0
8	0.0	0.0
9	0.0	0.0
>10	0.0	0.0
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.009184423218221896	0.0	0.0	0.0
3	0.0	0.009184423218221896	0.0	0.0	0.0
4	0.0	0.009184423218221896	0.0	0.0	0.0
5	0.0	0.009184423218221896	0.0	0.0	0.0
6	0.0	0.009184423218221896	0.0	0.0	0.0
7	0.0	0.009184423218221896	0.0	0.0	0.0
8	0.0	0.009184423218221896	0.0	0.0	0.0
9	0.0	0.009184423218221896	0.0	0.0	0.0
10	0.0	0.009184423218221896	0.0	0.0	0.0
11	0.0	0.009184423218221896	0.0	0.0	0.0
12	0.0	0.009184423218221896	0.0	0.0	0.0
13	0.0	0.009184423218221896	0.0	0.0	0.0
14	0.0	0.009184423218221896	0.0	0.0	0.0
15	0.0	0.009184423218221896	0.0	0.0	0.0
16	0.0	0.009184423218221896	0.0	0.0	0.0
17	0.0	0.009184423218221896	0.0	0.0	0.0
18	0.0	0.009184423218221896	0.0	0.0	0.0
19	0.0	0.009184423218221896	0.0	0.0	0.0
20	0.0	0.009184423218221896	0.0	0.0	0.0
21	0.0	0.009184423218221896	0.0	0.0	0.0
22	0.0	0.009184423218221896	0.0	0.0	0.0
23	0.0	0.009184423218221896	0.0	0.0	0.0
24	0.0	0.009184423218221896	0.0	0.0	0.0
25	0.0	0.009184423218221896	0.0	0.0	0.0
26	0.0	0.009184423218221896	0.0	0.0	0.0
27	0.0	0.009184423218221896	0.0	0.0	0.0
28	0.0	0.009184423218221896	0.0	0.009184423218221896	0.0
29	0.0	0.009184423218221896	0.0	0.04592211609110948	0.0
30	0.0	0.009184423218221896	0.0	0.06429096252755327	0.0
31	0.0	0.009184423218221896	0.0	0.07347538574577517	0.0
32	0.0	0.009184423218221896	0.0	0.08265980896399706	0.0
33	0.0	0.009184423218221896	0.0	0.11021307861866275	0.0
34	0.0	0.009184423218221896	0.0	0.14695077149155034	0.0
35	0.0	0.009184423218221896	0.0	0.18368846436443792	0.0
36	0.0	0.009184423218221896	0.0	0.2387950036737693	0.0
37	0.0	0.009184423218221896	0.0	0.349008082292432	0.0
38	0.0	0.009184423218221896	0.0	0.4867744305657605	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE