FastQCFastQC Report
Thu 2 Feb 2017
SRR4062320_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062320_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences151613
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT17461.1516162862023707No Hit
TATCAACGCAGAGTACTTTTTTTTT12090.7974250229201981No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA8900.587020901901552No Hit
ACGCAGAGTACTTTTTTTTTTTTTT7820.5157869048168693No Hit
GTACTTTTTTTTTTTTTTTTTTTTT7600.5012762757811006No Hit
GGTATCAACGCAGAGTACTTTTTTT6280.41421250156648837No Hit
GAGTACTTTTTTTTTTTTTTTTTTT5970.3937657061069961No Hit
CCATAGGGTCTTCTCGTCTTATTAT5640.37199976255334305No Hit
GTACATGGGGTGGTATCAACGCAAA3410.22491475005441486No Hit
CCATTGGGATGTCCTGATCCAACAT3370.22227645386609327No Hit
GTATTAGAGGCACTGCCTGCCCAGT3250.21436156530112854No Hit
GTCAGGATACCGCGGCCGTTAAACT3200.21106369506572656No Hit
GGTCAGGATACCGCGGCCGTTAAAC3060.201829658406601No Hit
ATCCTGACCGTGCAAAGGTAGCATA3010.19853178817119901No Hit
CTCTAATACTTGTAATGCTAGAGGT2950.19457434388871667No Hit
GTTCATGCTAGTCCCTAATTAAGGA2880.1899573255591539No Hit
GTATCAACGCAGAGTACATGGGGTG2830.18665945532375192No Hit
ACGCAGAGTACATGGGGTGGTATCA2780.18336158508834996No Hit
CCTATAACTTCTCTGTTAACCCAAC2740.18072328890002834No Hit
GACTATAGGCAATAATCACACTATA2730.18006371485294798No Hit
ACCTATAACTTCTCTGTTAACCCAA2710.1787445667587872No Hit
GCAGAGTACTTTTTTTTTTTTTTTT2690.17742541866462638No Hit
GCCTAAAGGAAAGATCCAAAAAGAT2630.173467974382144No Hit
CTTCTACACCATTGGGATGTCCTGA2630.173467974382144No Hit
CACTATAAATAATCCACCTATAACT2520.16621265986425965No Hit
ATAAATAATCCACCTATAACTTCTC2480.16357436367593808No Hit
ATCAACGCAGAGTACTTTTTTTTTT2450.16159564153469688No Hit
GTATCCTGACCGTGCAAAGGTAGCA2310.15236160487557138No Hit
CTGTTAGTATGAGTAACAAGAATTC2310.15236160487557138No Hit
CCTCTAGCATTACAAGTATTAGAGG2300.15170203082849096No Hit
ATCTATAACTTTATAGATGCAACAC2210.1457658644047674No Hit
GTTATATAATTTAAGCTCCATAGGG2150.14180842012228503No Hit
GTTATAGATTAACCCAATTTTAAGT2100.13851054988688305No Hit
TATCAACGCAGAGTACATGGGGTGG2080.13719140179272227No Hit
AGCATGAACGGCTAAACGAGGGTCC2000.13191480941607908No Hit
ATCGTAAATAGATAGAAACCGACCT1950.12861693918067713No Hit
CTATAGAACTAGTACCGCAAGGGAA1940.1279573651335967No Hit
GTATCAACGCAGAGTACATGGGAGA1920.12663821703943592No Hit
TGCTAGAGGTGATGTTTTTGGTAAA1920.12663821703943592No Hit
GATTAAAGATAAGAGACAGTTGGAC1890.12465949489819475No Hit
CTGTTAACCCAACACCGGAATGCCT1870.12334034680403394No Hit
GTACATGGGAGAAATCGTAAATAGA1870.12334034680403394No Hit
GATTAAACCTTGTACCTTTTGCATA1850.12202119870987317No Hit
GGATACCGCGGCCGTTAAACTTTAG1820.12004247656863197No Hit
GTCCTGATCCAACATCGAGGTCGTA1800.1187233284744712No Hit
GTTAGTATGAGTAACAAGAATTCCA1770.11674460633323No Hit
ACCTTTGCACGGTCAGGATACCGCG1770.11674460633323No Hit
ATTCCATTCCATTCCATTCCATTCC1760.1160850322861496No Hit
GCTATCACCAAGCTCGTTAGGCTTT1720.11344673609782802No Hit
GGGCAGGCAGTGCCTCTAATACTTG1680.11080843990950644No Hit
GGATTGCTCCGGTCTGAACTCAGAT1680.11080843990950644No Hit
TACTAACAGTGTTGCATCTATAAAG1670.11014886586242605No Hit
GATATACACTGTTCTACAAATCCCG1650.10882971776826525No Hit
ACCCTATGGAGCTTAAATTATATAA1640.10817014372118486No Hit
GATATATTTTGATCAACGGACCAAG1640.10817014372118486No Hit
GACCTGGATTGCTCCGGTCTGAACT1610.10619142157994367No Hit
GTTATCCCTAGGGTAACTTGGTCCG1600.10553184753286328No Hit
GTTTAAAATTGAACTTAAATTCATT1570.10355312539162209No Hit
CTATTAAAGGTTTTTTCCGTTCCAG1560.1028935513445417No Hit
AAATATATCTGGGTCAATAAGATAT1530.1009148292033005No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCATT307.676992E-419.000665
AGCATTA307.676992E-419.000666
CCTACAG504.506159E-617.1005943
ATAGATT504.506159E-617.1005944
AGGGTCC453.503899E-516.88947719
CGAGGGT453.503899E-516.88947717
CGGCTAA453.503899E-516.8894779
GCTAAAC453.503899E-516.88947711
ACGAGGG453.503899E-516.88947716
GATATAC453.5138553E-516.8838981
GTCCCCC402.7397813E-416.62557811
CTAGCAT350.002157546816.2862824
CTCAGAG350.002162318716.28092
CCATTAA350.002162318716.28091
TAGATTA601.4450488E-615.8338845
GTTATAT601.4500438E-615.8286521
GCATTTC551.1145745E-515.54599613
GAACGGC551.1145745E-515.5459966
AGTGTGC551.1145745E-515.5459968
GTCCTAC551.1180751E-515.5408591
Produced by FastQC (version 0.11.5)