##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062320_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	151613
Sequences flagged as poor quality	0
Sequence length	25
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.93935876211143	32.0	32.0	32.0	32.0	32.0
2	30.699346362119343	32.0	32.0	32.0	32.0	32.0
3	30.758516749882926	32.0	32.0	32.0	32.0	32.0
4	30.699682744883354	32.0	32.0	32.0	32.0	32.0
5	30.789444176950525	32.0	32.0	32.0	32.0	32.0
6	34.295753002710846	36.0	36.0	36.0	32.0	36.0
7	34.1848192437324	36.0	36.0	36.0	32.0	36.0
8	34.03900720914434	36.0	36.0	36.0	32.0	36.0
9	34.09378483375436	36.0	36.0	36.0	32.0	36.0
10	34.007848931160254	36.0	36.0	36.0	32.0	36.0
11	34.28312875544973	36.0	36.0	36.0	32.0	36.0
12	34.14620777901631	36.0	36.0	36.0	32.0	36.0
13	34.18845349673181	36.0	36.0	36.0	32.0	36.0
14	34.09885695817641	36.0	36.0	36.0	32.0	36.0
15	34.102702274870886	36.0	36.0	36.0	32.0	36.0
16	34.09565802404807	36.0	36.0	36.0	32.0	36.0
17	34.01065871660082	36.0	36.0	36.0	32.0	36.0
18	34.07763186534136	36.0	36.0	36.0	32.0	36.0
19	33.99664936384083	36.0	36.0	36.0	32.0	36.0
20	33.96045853587753	36.0	36.0	36.0	32.0	36.0
21	33.922598985575114	36.0	36.0	36.0	32.0	36.0
22	33.95305151932882	36.0	36.0	36.0	32.0	36.0
23	33.96037938699188	36.0	36.0	36.0	32.0	36.0
24	33.932288128326725	36.0	36.0	36.0	32.0	36.0
25	33.63530831788831	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	4.0
5	11.0
6	29.0
7	7.0
8	26.0
9	24.0
10	10.0
11	3.0
12	10.0
13	5.0
14	40.0
15	59.0
16	64.0
17	98.0
18	130.0
19	218.0
20	314.0
21	473.0
22	734.0
23	938.0
24	1207.0
25	1451.0
26	1872.0
27	2234.0
28	3027.0
29	3830.0
30	5048.0
31	7028.0
32	9782.0
33	14044.0
34	31418.0
35	67475.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.66614779832731	22.150621322850434	12.401393029575496	22.78183784924676
2	14.918905370911464	22.122113539076466	40.35405934847275	22.604921741539314
3	17.77629739070786	27.140991478247106	31.002163417143763	24.080547713901275
4	10.151524146893326	17.86164268798691	40.69581049256892	31.291022672550845
5	11.57782220550684	40.45936512335293	34.629215410703566	13.333597260436667
6	27.911320555467555	41.36174032419874	18.195918475104282	12.531020645229422
7	25.490623349181195	36.1918911780243	21.032091917591124	17.28539355520338
8	24.991416195657916	35.44252812846654	20.246553272410335	19.319502403465215
9	24.822044082883217	16.424109560095616	22.934852947002813	35.81899341001836
10	15.31486312452531	28.142522207178942	32.522537397219565	24.020077271076183
11	32.18404470336464	25.73944173635055	24.250650603046275	17.825862957238535
12	22.47554636778527	28.679272698452536	30.25645767424658	18.588723259515618
13	24.894495261367762	23.392662549945513	29.398672522537396	22.314169666149326
14	20.2575362366692	23.76927394591739	29.296397794433254	26.676792022980155
15	23.215158439774395	29.2350773376306	26.358857173048726	21.190907049546276
16	21.929523331528273	30.80382497061271	26.083367453409583	21.183284244449435
17	18.65808155852732	29.040118870728083	29.19597160310385	23.105827967640746
18	18.544563738890428	29.417085958031247	31.65616787501816	20.382182428060165
19	21.189753070117522	27.944011620229762	31.053743562656805	19.812491746995907
20	21.428477118487418	28.415722622725713	30.38237080461591	19.77342945417096
21	22.976630578294163	26.409426987060996	29.963031423290204	20.650911011354633
22	20.518948897398655	28.75742770368414	30.425194770896606	20.2984286280206
23	21.146089815102087	28.22647188904805	30.80619714962803	19.821241146221837
24	20.2095353780747	28.809464080592562	30.484294749072472	20.49670579226026
25	20.97313996395778	28.420545386133643	30.979147000772333	19.62716764913624
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	988.0
1	988.0
2	614.5
3	241.0
4	241.0
5	241.0
6	706.5
7	1172.0
8	1172.0
9	1172.0
10	1169.0
11	1166.0
12	1166.0
13	1166.0
14	1155.5
15	1145.0
16	1145.0
17	1145.0
18	1908.0
19	2671.0
20	2671.0
21	2671.0
22	4149.5
23	5628.0
24	5628.0
25	5628.0
26	8352.0
27	11076.0
28	11076.0
29	11076.0
30	12799.0
31	14522.0
32	14522.0
33	14522.0
34	15249.0
35	15976.0
36	15976.0
37	15976.0
38	15959.0
39	15942.0
40	15942.0
41	15942.0
42	15327.0
43	14712.0
44	14712.0
45	14712.0
46	16379.0
47	18046.0
48	18046.0
49	18046.0
50	17475.0
51	16904.0
52	16904.0
53	16904.0
54	14453.0
55	12002.0
56	12002.0
57	12002.0
58	9853.0
59	7704.0
60	7704.0
61	7704.0
62	6302.5
63	4901.0
64	4901.0
65	4901.0
66	4100.0
67	3299.0
68	3299.0
69	3299.0
70	2499.0
71	1699.0
72	1699.0
73	1699.0
74	1383.0
75	1067.0
76	1067.0
77	1067.0
78	760.0
79	453.0
80	453.0
81	453.0
82	296.5
83	140.0
84	140.0
85	140.0
86	101.5
87	63.0
88	63.0
89	63.0
90	38.5
91	14.0
92	14.0
93	14.0
94	12.0
95	10.0
96	10.0
97	10.0
98	41.0
99	72.0
100	72.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	6.595740470803955E-4
2	0.0
3	6.595740470803955E-4
4	0.01319148094160791
5	0.036936146636502146
6	0.06661697875511995
7	0.11410631014490842
8	0.10882971776826525
9	0.11278716205074762
10	0.13059566132191833
11	0.14180842012228503
12	0.13455310560440067
13	0.13059566132191833
14	0.11740418038031039
15	0.13125523536899872
16	0.12334034680403394
17	0.12399992085111436
18	0.11014886586242605
19	0.1009148292033005
20	0.09036164445001417
21	0.08772334826169259
22	0.1009148292033005
23	0.08178718183796904
24	0.08904249635585339
25	0.08178718183796904
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	151613.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.797550341989144
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.19879244756221	25.777472907996017
2	14.843122884165707	11.220673688931688
3	6.097092800055841	6.913655161496705
4	3.1916378738701003	4.825443728440174
5	1.884619411579939	3.561699854234136
6	1.1656720064216661	2.643572780698225
7	0.8306285554741213	2.197700724871878
8	0.5688758594213521	1.7201691147856715
9	0.4275294035528566	1.4543607738122721
>10	2.3365790667643873	16.71162763087598
>50	0.26698774997382474	6.914974309590866
>100	0.1745017973685129	11.325545962417472
>500	0.010470107842110775	2.784062052726349
>1k	0.0034900359473702576	1.9490413091225685
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	1746	1.1516162862023707	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1209	0.7974250229201981	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	890	0.587020901901552	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	782	0.5157869048168693	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	760	0.5012762757811006	No Hit
GGTATCAACGCAGAGTACTTTTTTT	628	0.41421250156648837	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	597	0.3937657061069961	No Hit
CCATAGGGTCTTCTCGTCTTATTAT	564	0.37199976255334305	No Hit
GTACATGGGGTGGTATCAACGCAAA	341	0.22491475005441486	No Hit
CCATTGGGATGTCCTGATCCAACAT	337	0.22227645386609327	No Hit
GTATTAGAGGCACTGCCTGCCCAGT	325	0.21436156530112854	No Hit
GTCAGGATACCGCGGCCGTTAAACT	320	0.21106369506572656	No Hit
GGTCAGGATACCGCGGCCGTTAAAC	306	0.201829658406601	No Hit
ATCCTGACCGTGCAAAGGTAGCATA	301	0.19853178817119901	No Hit
CTCTAATACTTGTAATGCTAGAGGT	295	0.19457434388871667	No Hit
GTTCATGCTAGTCCCTAATTAAGGA	288	0.1899573255591539	No Hit
GTATCAACGCAGAGTACATGGGGTG	283	0.18665945532375192	No Hit
ACGCAGAGTACATGGGGTGGTATCA	278	0.18336158508834996	No Hit
CCTATAACTTCTCTGTTAACCCAAC	274	0.18072328890002834	No Hit
GACTATAGGCAATAATCACACTATA	273	0.18006371485294798	No Hit
ACCTATAACTTCTCTGTTAACCCAA	271	0.1787445667587872	No Hit
GCAGAGTACTTTTTTTTTTTTTTTT	269	0.17742541866462638	No Hit
GCCTAAAGGAAAGATCCAAAAAGAT	263	0.173467974382144	No Hit
CTTCTACACCATTGGGATGTCCTGA	263	0.173467974382144	No Hit
CACTATAAATAATCCACCTATAACT	252	0.16621265986425965	No Hit
ATAAATAATCCACCTATAACTTCTC	248	0.16357436367593808	No Hit
ATCAACGCAGAGTACTTTTTTTTTT	245	0.16159564153469688	No Hit
GTATCCTGACCGTGCAAAGGTAGCA	231	0.15236160487557138	No Hit
CTGTTAGTATGAGTAACAAGAATTC	231	0.15236160487557138	No Hit
CCTCTAGCATTACAAGTATTAGAGG	230	0.15170203082849096	No Hit
ATCTATAACTTTATAGATGCAACAC	221	0.1457658644047674	No Hit
GTTATATAATTTAAGCTCCATAGGG	215	0.14180842012228503	No Hit
GTTATAGATTAACCCAATTTTAAGT	210	0.13851054988688305	No Hit
TATCAACGCAGAGTACATGGGGTGG	208	0.13719140179272227	No Hit
AGCATGAACGGCTAAACGAGGGTCC	200	0.13191480941607908	No Hit
ATCGTAAATAGATAGAAACCGACCT	195	0.12861693918067713	No Hit
CTATAGAACTAGTACCGCAAGGGAA	194	0.1279573651335967	No Hit
GTATCAACGCAGAGTACATGGGAGA	192	0.12663821703943592	No Hit
TGCTAGAGGTGATGTTTTTGGTAAA	192	0.12663821703943592	No Hit
GATTAAAGATAAGAGACAGTTGGAC	189	0.12465949489819475	No Hit
CTGTTAACCCAACACCGGAATGCCT	187	0.12334034680403394	No Hit
GTACATGGGAGAAATCGTAAATAGA	187	0.12334034680403394	No Hit
GATTAAACCTTGTACCTTTTGCATA	185	0.12202119870987317	No Hit
GGATACCGCGGCCGTTAAACTTTAG	182	0.12004247656863197	No Hit
GTCCTGATCCAACATCGAGGTCGTA	180	0.1187233284744712	No Hit
GTTAGTATGAGTAACAAGAATTCCA	177	0.11674460633323	No Hit
ACCTTTGCACGGTCAGGATACCGCG	177	0.11674460633323	No Hit
ATTCCATTCCATTCCATTCCATTCC	176	0.1160850322861496	No Hit
GCTATCACCAAGCTCGTTAGGCTTT	172	0.11344673609782802	No Hit
GGGCAGGCAGTGCCTCTAATACTTG	168	0.11080843990950644	No Hit
GGATTGCTCCGGTCTGAACTCAGAT	168	0.11080843990950644	No Hit
TACTAACAGTGTTGCATCTATAAAG	167	0.11014886586242605	No Hit
GATATACACTGTTCTACAAATCCCG	165	0.10882971776826525	No Hit
ACCCTATGGAGCTTAAATTATATAA	164	0.10817014372118486	No Hit
GATATATTTTGATCAACGGACCAAG	164	0.10817014372118486	No Hit
GACCTGGATTGCTCCGGTCTGAACT	161	0.10619142157994367	No Hit
GTTATCCCTAGGGTAACTTGGTCCG	160	0.10553184753286328	No Hit
GTTTAAAATTGAACTTAAATTCATT	157	0.10355312539162209	No Hit
CTATTAAAGGTTTTTTCCGTTCCAG	156	0.1028935513445417	No Hit
AAATATATCTGGGTCAATAAGATAT	153	0.1009148292033005	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.001319148094160791	0.0
8	0.0	0.0	0.0	0.001319148094160791	0.0
9	0.0	0.0	0.0	0.0019787221412411863	0.0
10	0.0	0.0	0.0	0.0019787221412411863	0.0
11	0.0	0.0	0.0	0.0019787221412411863	0.0
12	0.0	0.0	0.0	0.0019787221412411863	0.0
13	0.0	0.0	0.0	0.0019787221412411863	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGCATT	30	7.676992E-4	19.00066	5
AGCATTA	30	7.676992E-4	19.00066	6
CCTACAG	50	4.506159E-6	17.100594	3
ATAGATT	50	4.506159E-6	17.100594	4
AGGGTCC	45	3.503899E-5	16.889477	19
CGAGGGT	45	3.503899E-5	16.889477	17
CGGCTAA	45	3.503899E-5	16.889477	9
GCTAAAC	45	3.503899E-5	16.889477	11
ACGAGGG	45	3.503899E-5	16.889477	16
GATATAC	45	3.5138553E-5	16.883898	1
GTCCCCC	40	2.7397813E-4	16.625578	11
CTAGCAT	35	0.0021575468	16.286282	4
CTCAGAG	35	0.0021623187	16.2809	2
CCATTAA	35	0.0021623187	16.2809	1
TAGATTA	60	1.4450488E-6	15.833884	5
GTTATAT	60	1.4500438E-6	15.828652	1
GCATTTC	55	1.1145745E-5	15.545996	13
GAACGGC	55	1.1145745E-5	15.545996	6
AGTGTGC	55	1.1145745E-5	15.545996	8
GTCCTAC	55	1.1180751E-5	15.540859	1
>>END_MODULE