##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062320_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	151613
Sequences flagged as poor quality	0
Sequence length	50
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.95693641046612	32.0	32.0	32.0	32.0	32.0
2	31.35629530449236	32.0	32.0	32.0	32.0	32.0
3	31.511334779999075	32.0	32.0	32.0	32.0	32.0
4	31.62951066201447	32.0	32.0	32.0	32.0	32.0
5	31.60703897423044	32.0	32.0	32.0	32.0	32.0
6	35.16460989492985	36.0	36.0	36.0	36.0	36.0
7	35.16402287402795	36.0	36.0	36.0	36.0	36.0
8	35.11550460712472	36.0	36.0	36.0	36.0	36.0
9	35.15658287877689	36.0	36.0	36.0	36.0	36.0
10	35.08565888149433	36.0	36.0	36.0	36.0	36.0
11	35.206433485255225	36.0	36.0	36.0	36.0	36.0
12	35.12016779563758	36.0	36.0	36.0	36.0	36.0
13	35.13397268044297	36.0	36.0	36.0	36.0	36.0
14	35.090592495366494	36.0	36.0	36.0	36.0	36.0
15	35.1184001371914	36.0	36.0	36.0	36.0	36.0
16	35.10476014589778	36.0	36.0	36.0	36.0	36.0
17	35.0982501500531	36.0	36.0	36.0	36.0	36.0
18	35.09330994044046	36.0	36.0	36.0	36.0	36.0
19	35.02081615692586	36.0	36.0	36.0	36.0	36.0
20	34.95332854042859	36.0	36.0	36.0	36.0	36.0
21	34.92582430266534	36.0	36.0	36.0	36.0	36.0
22	34.959403217402205	36.0	36.0	36.0	36.0	36.0
23	34.9823102240573	36.0	36.0	36.0	36.0	36.0
24	34.94056578261758	36.0	36.0	36.0	36.0	36.0
25	34.97465916511117	36.0	36.0	36.0	36.0	36.0
26	34.939662166173086	36.0	36.0	36.0	36.0	36.0
27	34.946132587574944	36.0	36.0	36.0	36.0	36.0
28	34.90123538219018	36.0	36.0	36.0	32.0	36.0
29	34.88158007558719	36.0	36.0	36.0	32.0	36.0
30	34.87538007954463	36.0	36.0	36.0	32.0	36.0
31	34.854774986313835	36.0	36.0	36.0	32.0	36.0
32	34.80626331515108	36.0	36.0	36.0	32.0	36.0
33	34.767440786739925	36.0	36.0	36.0	32.0	36.0
34	34.72513570736019	36.0	36.0	36.0	32.0	36.0
35	34.71948315777671	36.0	36.0	36.0	32.0	36.0
36	34.68935381530608	36.0	36.0	36.0	32.0	36.0
37	34.690138708422104	36.0	36.0	36.0	32.0	36.0
38	34.67512680311055	36.0	36.0	36.0	32.0	36.0
39	34.598128128854384	36.0	36.0	36.0	32.0	36.0
40	34.57645452566732	36.0	36.0	36.0	32.0	36.0
41	34.52719753583136	36.0	36.0	36.0	32.0	36.0
42	34.513880735820806	36.0	36.0	36.0	32.0	36.0
43	34.465778000567234	36.0	36.0	36.0	32.0	36.0
44	34.352522540943056	36.0	36.0	36.0	32.0	36.0
45	34.25825621813433	36.0	36.0	36.0	32.0	36.0
46	34.15456458219282	36.0	36.0	36.0	32.0	36.0
47	34.11156695006365	36.0	36.0	36.0	32.0	36.0
48	34.036157849260945	36.0	36.0	36.0	32.0	36.0
49	33.95521492220324	36.0	36.0	36.0	32.0	36.0
50	33.540593484727566	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	0.0
20	1.0
21	3.0
22	15.0
23	39.0
24	84.0
25	239.0
26	467.0
27	930.0
28	1556.0
29	2625.0
30	3903.0
31	5607.0
32	7975.0
33	12436.0
34	24940.0
35	90792.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.024372220682736	22.953696739374266	13.053719171845929	22.968211868097065
2	14.401810038324792	22.467826304922855	40.45475234005501	22.67561131669734
3	17.13045715077203	27.576790908431338	31.488065007618083	23.804686933178555
4	10.011674460633323	18.09277568546233	40.78212290502793	31.113426948876416
5	11.154056710176569	40.82433564404108	34.54782901202403	13.473778633758318
6	27.95754953433765	41.29620346674406	18.440492836978603	12.305754161939689
7	25.853323923410258	35.969211083482286	21.01534828807556	17.16211670503189
8	25.416685904243042	35.31425405473145	20.072157400750594	19.19690264027491
9	25.727345280417907	15.951798328639363	22.38462400981446	35.93623238112827
10	15.7117133755021	28.0187055199752	32.28219216030288	23.987388944219823
11	32.565809000547446	25.596749619095988	24.288154709688484	17.54928667066808
12	22.744751439520357	28.677619993008513	30.023151049052522	18.554477518418604
13	25.570366657212773	23.339027655939795	28.903853891157087	22.186751795690345
14	21.0140291399814	23.56196368385297	28.728407194633704	26.695599981531927
15	23.307368101679934	29.349066372936356	25.94896216023692	21.39460336514679
16	22.028889914913265	30.67805553723369	25.929028428203942	21.3640261196491
17	18.99837085210371	29.008726164642873	28.65585404945486	23.337048933798556
18	19.016838925421965	29.38402379743162	31.141129058853796	20.458008218292626
19	21.708560611557058	27.743003568295592	30.767150574159206	19.78128524598814
20	22.34783526369944	28.42321188296441	29.453473339841175	19.77547951349497
21	23.988708092313985	26.10528120939497	29.246172821591816	20.65983787669923
22	21.04964613852374	28.81415182075416	29.484279052587837	20.651922988134263
23	21.47902884317308	28.256811750971224	30.212448800564594	20.051710605291102
24	20.828963597628142	28.840255654273108	29.675946995930374	20.65483375216838
25	21.76726270174721	28.362343598504086	30.174853079881014	19.695540619867693
26	19.801732041447632	28.865598596426427	31.286894923258558	20.04577443886738
27	19.38092379941034	30.20057646771715	30.335129573321552	20.083370159550963
28	19.214178297232408	29.725219639606365	30.429649368123897	20.630952695037333
29	19.747117642402976	28.830831332612195	30.01873202648867	21.40331899849616
30	21.145423845078227	28.52214864258766	30.324776402923252	20.007651109410865
31	20.642825106192124	28.795214099147827	29.685644935757065	20.876315858902988
32	18.641004669815054	29.5649420890167	30.558926734031605	21.23512650713664
33	20.307625335558296	29.47570458997579	30.08976802780764	20.12690204665827
34	19.704375012367176	28.745275738567784	31.23915810858051	20.31119114048453
35	21.084222121086203	29.611967469378868	28.7307649181128	20.573045491422125
36	20.347333984117352	29.772049705828035	29.302429886816345	20.578186423238265
37	20.852846118329925	29.47694743090825	28.27583932458281	21.394367126179013
38	19.918476647780864	30.423512495630323	29.797576724951025	19.86043413163779
39	19.575096793768264	30.345423485103325	28.292801973471583	21.78667774765683
40	20.374242314313417	30.708448483969054	29.68676828504152	19.230540916676013
41	20.95178418310138	30.255919794208825	29.56401292790713	19.228283094782668
42	19.55304314558419	31.273127840478093	28.77053884157965	20.403290172358066
43	18.893176346426568	31.587348702219586	29.058408363840243	20.461066587513603
44	20.05791327520019	29.767951136498557	29.35108109177737	20.823054496523884
45	20.364495043104867	30.122619667957284	29.17939145290125	20.333493836036595
46	22.37231790088848	30.383161727360875	26.88002532864577	20.364495043104867
47	21.013785370358157	31.085680364092077	27.327353076973814	20.57318118857595
48	20.294845156822007	31.39210448204215	27.571650011543152	20.74140034959269
49	19.89736689356762	29.95422405150124	28.619861748720382	21.528547306210754
50	19.451480132974513	30.459474434066802	28.311170914463617	21.777874518495068
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	592.0
1	313.5
2	35.0
3	92.0
4	149.0
5	139.5
6	130.0
7	95.0
8	60.0
9	93.5
10	127.0
11	209.0
12	291.0
13	586.5
14	882.0
15	1138.0
16	1394.0
17	1610.5
18	1827.0
19	1889.0
20	1951.0
21	2215.5
22	2480.0
23	2415.5
24	2351.0
25	2800.0
26	3249.0
27	4116.5
28	4984.0
29	5725.0
30	6466.0
31	6302.5
32	6139.0
33	6636.0
34	7133.0
35	8010.0
36	8887.0
37	10402.0
38	11917.0
39	11607.0
40	11297.0
41	11363.5
42	11430.0
43	10171.5
44	8913.0
45	8579.0
46	8245.0
47	7776.0
48	7307.0
49	6942.5
50	6578.0
51	6792.5
52	7007.0
53	6516.5
54	6026.0
55	5626.0
56	5226.0
57	5225.5
58	5225.0
59	4339.5
60	3454.0
61	3029.0
62	2604.0
63	2285.5
64	1967.0
65	1706.0
66	1445.0
67	1360.0
68	1275.0
69	1134.5
70	994.0
71	798.5
72	603.0
73	526.0
74	449.0
75	335.5
76	222.0
77	175.5
78	129.0
79	110.5
80	92.0
81	62.5
82	33.0
83	34.5
84	36.0
85	21.5
86	7.0
87	5.0
88	3.0
89	1.5
90	0.0
91	0.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03099998021277859
2	0.009234036659125536
3	0.0
4	0.0
5	0.0
6	6.595740470803955E-4
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0019787221412411863
17	0.0
18	0.0
19	0.0
20	6.595740470803955E-4
21	0.0
22	0.0
23	0.0
24	0.001319148094160791
25	0.0
26	0.0
27	0.0
28	6.595740470803955E-4
29	6.595740470803955E-4
30	6.595740470803955E-4
31	6.595740470803955E-4
32	6.595740470803955E-4
33	0.0
34	0.001319148094160791
35	0.001319148094160791
36	6.595740470803955E-4
37	0.0019787221412411863
38	0.0
39	0.001319148094160791
40	0.0
41	0.0019787221412411863
42	0.006595740470803955
43	0.005276592376643164
44	0.004617018329562768
45	0.003957444282482373
46	0.003957444282482373
47	0.0019787221412411863
48	0.005276592376643164
49	0.0032978702354019775
50	0.0032978702354019775
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	151613.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.49371755720156
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.881724769503	29.695342747653562
2	14.576413249309287	12.388119752263988
3	6.020861143016795	7.675463185874562
4	3.039145686524074	5.165783936733657
5	1.5366466954335207	3.2648915330479573
6	1.0663396765281097	2.7187642220653903
7	0.6612237295501816	1.9668498083937396
8	0.5075590600068295	1.7254457071623146
9	0.3585508956011548	1.3712544438801422
>10	1.9929841989258994	16.29081938883869
>50	0.22196007822928632	6.501421382071458
>100	0.13503864899264273	10.886929221109009
>500	0.0015521683792257784	0.3489146709055292
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCATAGGGTCTTCTCGTCTTATTATTATATTTCAGCCTCTTCACTGAAAG	529	0.3489146709055292	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	479	0.31593596855150946	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	402	0.26514876692631895	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	388	0.2559147302671934	No Hit
ACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA	340	0.22425517600733447	No Hit
GTATTAGAGGCACTGCCTGCCCAGTGACTAAAGTTTAACGGCCGCGGTAT	334	0.2202977317248521	No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA	316	0.20842539887740494	No Hit
GTCAGGATACCGCGGCCGTTAAACTTTAGTCACTGGGCAGGCAGTGCCTC	307	0.2024892324536814	No Hit
GTTCATGCTAGTCCCTAATTAAGGAACAAGTGATTATGCTACCTTTGCAC	304	0.20051051031244024	No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAA	298	0.19655306602995784	No Hit
CTCTAATACTTGTAATGCTAGAGGTGATGTTTTTGGTAAACAGGCGGGGT	296	0.19523391793579706	No Hit
ATAAATAATCCACCTATAACTTCTCTGTTAACCCAACACCGGAATGCCTA	287	0.1892977515120735	No Hit
CCATTGGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTAATTGTCGA	285	0.1879786034179127	No Hit
CACTATAAATAATCCACCTATAACTTCTCTGTTAACCCAACACCGGAATG	277	0.18270201104126954	No Hit
GGTCAGGATACCGCGGCCGTTAAACTTTAGTCACTGGGCAGGCAGTGCCT	276	0.18204243699418915	No Hit
GACTATAGGCAATAATCACACTATAAATAATCCACCTATAACTTCTCTGT	269	0.17742541866462638	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	268	0.176765844617546	No Hit
GCCTAAAGGAAAGATCCAAAAAGATAAAAGGAACTCGGCAAACAAGAACC	268	0.176765844617546	No Hit
CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA	265	0.1747871224763048	No Hit
ATCCTGACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGACT	262	0.17280840033506362	No Hit
CTTCTACACCATTGGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTA	246	0.1622552155817773	No Hit
CCTATAACTTCTCTGTTAACCCAACACCGGAATGCCTAAAGGAAAGATCC	241	0.1589573453463753	No Hit
ACCTATAACTTCTCTGTTAACCCAACACCGGAATGCCTAAAGGAAAGATC	240	0.15829777129929493	No Hit
CCTCTAGCATTACAAGTATTAGAGGCACTGCCTGCCCAGTGACTAAAGTT	238	0.15697862320513414	No Hit
GTACATGGGAGAAATCGTAAATAGATAGAAACCGACCTGGATTGCTCCGG	233	0.15368075296973213	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	228	0.15038288273433018	No Hit
GTTATATAATTTAAGCTCCATAGGGTCTTCTCGTCTTATTATTATATTTC	217	0.14312756821644582	No Hit
ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC	213	0.14048927202812425	No Hit
GTATCCTGACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGA	211	0.13917012393396344	No Hit
GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC	210	0.13851054988688305	No Hit
TGCTAGAGGTGATGTTTTTGGTAAACAGGCGGGGTTCTTGTTTGCCGAGT	206	0.13587225369856148	No Hit
GATTAAAGATAAGAGACAGTTGGACCCTCGTTTAGCCGTTCATGCTAGTC	204	0.13455310560440067	No Hit
CTGTTAACCCAACACCGGAATGCCTAAAGGAAAGATCCAAAAAGATAAAA	202	0.1332339575102399	No Hit
ATCGTAAATAGATAGAAACCGACCTGGATTGCTCCGGTCTGAACTCAGAT	199	0.13125523536899872	No Hit
GATATATTTTGATCAACGGACCAAGTTACCCTAGGGATAACAGCGCAATC	199	0.13125523536899872	No Hit
GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA	195	0.12861693918067713	No Hit
AGCATGAACGGCTAAACGAGGGTCCAACTGTCTCTTATCTTTAATCAGTG	193	0.12729779108651632	No Hit
ACCTTTGCACGGTCAGGATACCGCGGCCGTTAAACTTTAGTCACTGGGCA	190	0.12531906894527514	No Hit
GTATCAACGCAGAGTACATGGGAGAAATCGTAAATAGATAGAAACCGACC	184	0.12136162466279278	No Hit
TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT	184	0.12136162466279278	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	179	0.11806375442739078	No Hit
CTATTAAAGGTTTTTTCCGTTCCAGAAGAGCTGTCCCTCTTTTGGCTATA	178	0.11740418038031039	No Hit
AAATATATCTGGGTCAATAAGATATGTTGATTTTACTTTGACTTGTAAGT	177	0.11674460633323	No Hit
GGATACCGCGGCCGTTAAACTTTAGTCACTGGGCAGGCAGTGCCTCTAAT	173	0.11410631014490842	No Hit
GTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAACA	172	0.11344673609782802	No Hit
CTATAGAACTAGTACCGCAAGGGAAAGATGAAAGACTAATTAAAAGTAAG	171	0.11278716205074762	No Hit
ACCCTATGGAGCTTAAATTATATAACTTATCTATTTAATTTATTAAACCT	169	0.11146801395658684	No Hit
GGGCAGGCAGTGCCTCTAATACTTGTAATGCTAGAGGTGATGTTTTTGGT	166	0.10948929181534565	No Hit
GATAGAAACCGACCTGGATTGCTCCGGTCTGAACTCAGATCACGTAGGAC	157	0.10355312539162209	No Hit
GTTATCCCTAGGGTAACTTGGTCCGTTGATCAAAATATATCTGGGTCAAT	156	0.1028935513445417	No Hit
CTTTATTGGTGGCTGCTTTTAGGCCTACAATGGTTAAAAGCTGTTTTGTT	153	0.1009148292033005	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	6.595740470803955E-4	0.0
8	0.0	0.0	0.0	6.595740470803955E-4	0.0
9	0.0	0.0	0.0	6.595740470803955E-4	0.0
10	0.0	0.0	0.0	6.595740470803955E-4	0.0
11	0.0	0.0	0.0	6.595740470803955E-4	0.0
12	0.0	0.0	0.0	6.595740470803955E-4	0.0
13	0.0	0.0	0.0	6.595740470803955E-4	0.0
14	0.0	0.0	0.0	6.595740470803955E-4	0.0
15	0.0	0.0	0.0	6.595740470803955E-4	0.0
16	0.0	0.0	0.0	6.595740470803955E-4	0.0
17	0.0	0.0	0.0	6.595740470803955E-4	0.0
18	0.0	0.0	0.0	6.595740470803955E-4	0.0
19	0.0	0.0	0.0	6.595740470803955E-4	0.0
20	0.0	0.0	0.0	0.001319148094160791	0.0
21	0.0	0.0	0.0	0.001319148094160791	0.0
22	0.0	0.0	0.0	0.001319148094160791	0.0
23	0.0	0.0	0.0	0.005276592376643164	0.0
24	0.0	0.0	0.0	0.0072553145178843505	0.0
25	0.0	0.0	0.0	0.007914888564964745	0.0
26	0.0	0.0	0.0	0.008574462612045142	0.0
27	0.0	0.0	0.0	0.009893610706205933	0.0
28	0.0	0.0	0.0	0.015170203082849096	0.0
29	0.0	0.0	0.0	0.021106369506572655	0.0
30	0.0	0.0	0.0	0.03957444282482373	0.0
31	0.0	0.0	0.0	0.07387229327300429	0.0
32	0.0	0.0	0.0	0.10091482920330051	0.0
33	0.0	0.0	0.0	0.12861693918067713	0.0
34	0.0	0.0	0.0	0.15368075296973216	0.0
35	0.0	0.0	0.0	0.19127647365331468	0.0
36	0.0	0.0	0.0	0.22887219433689723	0.0
37	0.0	0.0	0.0	0.28427641429165046	0.0
38	0.0	0.0	0.0	0.34297850448180567	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACCGA	25	0.0023463285	35.2	6
GACGTGG	25	0.0023463285	35.2	7
GTTTTAC	25	0.0023463285	35.2	1
GTCCACC	25	0.0023463285	35.2	1
GTCCTAC	65	1.7256752E-8	30.46154	1
CCTACAG	45	4.046387E-5	29.333334	3
GTGCTGG	45	4.046387E-5	29.333334	1
CGTGATT	30	0.005729382	29.333332	28
GTCCTAA	30	0.005729382	29.333332	1
AGGTTAA	30	0.005729382	29.333332	1
CCTTTAG	30	0.005729382	29.333332	43
TCCGTGA	30	0.005729382	29.333332	26
CATGTGC	30	0.005729382	29.333332	18
TAGGACG	60	2.9167495E-7	29.333332	4
TGTCGCC	30	0.005729382	29.333332	18
ATACATG	60	2.9167495E-7	29.333332	1
TATGGCA	40	7.003609E-4	27.5	16
TCCTACA	50	8.294283E-5	26.400002	2
TGGCAAG	50	8.294283E-5	26.400002	18
GATATAC	60	9.679767E-6	25.666666	1
>>END_MODULE