FastQCFastQC Report
Thu 2 Feb 2017
SRR4062319_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062319_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences987013
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT21130.21408026034104918No Hit
TATCAACGCAGAGTACTTTTTTTTT12890.13059605091320986No Hit
GCGCAAGACGGACCAGAGCGAAAGC11800.11955263000588644No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCGCG400.00527177514.2522467
CCAACCG551.9555484E-413.821069
CGTTATT802.0017214E-613.059922
TTCGAAG802.0017214E-613.059922
GCGTTAT802.0074294E-613.0566081
TTTTCGG957.385279E-812.9987516
CGAGCCG1850.012.83790715
ATCGAAC604.082485E-412.6693068
CGAACCC604.084233E-412.66866310
CGAAGAC751.4772026E-512.6667344
AAGACGG1850.012.3256425
TCTAGCC701.0900652E-412.2143513
GTATAGA701.09472116E-412.2087761
GCGGTCG951.0358708E-612.00259
TAGAACC951.0384138E-612.0000644
CGAGGTT951.0384138E-612.0000644
CCGAGGT1209.991709E-911.8750633
GTATTAG1052.731722E-711.7565981
AGACGAT658.008259E-411.694157
AATCGAA658.008259E-411.694157
Produced by FastQC (version 0.11.5)