##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062319_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	987013
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.214904970856512	32.0	32.0	32.0	32.0	32.0
2	30.791358371166336	32.0	32.0	32.0	32.0	32.0
3	30.822001331289457	32.0	32.0	32.0	32.0	32.0
4	30.82414517336651	32.0	32.0	32.0	32.0	32.0
5	30.778245068707303	32.0	32.0	32.0	32.0	32.0
6	34.417123178722065	36.0	36.0	36.0	32.0	36.0
7	34.3376176402945	36.0	36.0	36.0	32.0	36.0
8	34.30459983809737	36.0	36.0	36.0	32.0	36.0
9	34.41189123142249	36.0	36.0	36.0	32.0	36.0
10	34.134413629810346	36.0	36.0	36.0	32.0	36.0
11	34.40327331048324	36.0	36.0	36.0	32.0	36.0
12	34.23826737844385	36.0	36.0	36.0	32.0	36.0
13	34.319016061591896	36.0	36.0	36.0	32.0	36.0
14	34.221945404974406	36.0	36.0	36.0	32.0	36.0
15	34.193837365870564	36.0	36.0	36.0	32.0	36.0
16	34.1900866553936	36.0	36.0	36.0	32.0	36.0
17	34.116242643207336	36.0	36.0	36.0	32.0	36.0
18	34.13232449825889	36.0	36.0	36.0	32.0	36.0
19	34.12400241942102	36.0	36.0	36.0	32.0	36.0
20	34.11012924855093	36.0	36.0	36.0	32.0	36.0
21	34.098916630277415	36.0	36.0	36.0	32.0	36.0
22	34.0664641701781	36.0	36.0	36.0	32.0	36.0
23	34.039058249486075	36.0	36.0	36.0	32.0	36.0
24	34.01780422344994	36.0	36.0	36.0	32.0	36.0
25	33.67270339904338	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	7.0
5	87.0
6	183.0
7	43.0
8	180.0
9	142.0
10	69.0
11	20.0
12	45.0
13	47.0
14	210.0
15	321.0
16	416.0
17	566.0
18	795.0
19	1001.0
20	1568.0
21	2226.0
22	3228.0
23	4586.0
24	6447.0
25	8602.0
26	11694.0
27	14810.0
28	18965.0
29	24951.0
30	32568.0
31	43563.0
32	61637.0
33	88420.0
34	199199.0
35	460417.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.70333198582789	17.215648489527453	11.172238551016251	25.90878097362841
2	16.980424776573358	19.434698428490808	36.976919250303695	26.60795754463214
3	19.677165410174396	22.6409741774881	27.969001233020634	29.712859179316865
4	13.091934142117898	15.269735022236002	34.81360565723625	36.82472517840985
5	15.118309582229896	36.21164072642803	33.05067138546921	15.61937830587286
6	35.131436218205415	34.402935898865586	16.359931468659077	14.105696414269927
7	30.88373706352716	30.09649361239467	19.812834441546006	19.206934882532163
8	28.37692139948374	32.88867927398865	18.87761363578735	19.85678569074026
9	26.79296689563951	14.756271732471	18.554610631210362	39.896150740679126
10	16.559265015602996	26.773750441307158	30.808819650444146	25.85816489264569
11	37.33274622880032	21.437398288197983	21.443282861282352	19.78657262171935
12	24.549124321670135	23.835108903076314	28.168709730108667	23.447057045144888
13	29.545721995658088	19.445797066163493	24.751962992269767	26.25651794590866
14	23.386372834524668	19.587997999734245	24.903410087952864	32.12221907778823
15	25.193290798028993	26.9753908679292	21.94592422980427	25.885394104237534
16	25.75966129305474	26.008072082745443	23.230212594599475	25.00205402960034
17	24.07894136204789	26.189014957470874	24.82909732753767	24.902946352943562
18	24.78223296913733	25.23227130911761	25.63240681761935	24.353088904125705
19	25.48510558031142	25.254088435553516	25.110285840616424	24.15052014351864
20	25.54565468928891	24.531568011244193	24.83802500534931	25.084752294117585
21	26.332495352910097	24.481163694185483	24.653559828050067	24.53278112485435
22	25.947096665574698	24.405899874947643	24.83866420145499	24.80833925802267
23	24.47843099876598	24.69572817876977	25.346605732873524	25.479235089590723
24	24.64773378358508	25.057422308757094	25.32939870096287	24.96544520669496
25	24.776300418600123	24.725192264010122	25.130001297984876	25.368506019404872
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	521.0
1	521.0
2	488.0
3	455.0
4	455.0
5	455.0
6	896.0
7	1337.0
8	1337.0
9	1337.0
10	1593.0
11	1849.0
12	1849.0
13	1849.0
14	2341.5
15	2834.0
16	2834.0
17	2834.0
18	4566.5
19	6299.0
20	6299.0
21	6299.0
22	9693.0
23	13087.0
24	13087.0
25	13087.0
26	18906.5
27	24726.0
28	24726.0
29	24726.0
30	31192.0
31	37658.0
32	37658.0
33	37658.0
34	47448.5
35	57239.0
36	57239.0
37	57239.0
38	67400.0
39	77561.0
40	77561.0
41	77561.0
42	89973.0
43	102385.0
44	102385.0
45	102385.0
46	114524.0
47	126663.0
48	126663.0
49	126663.0
50	131704.0
51	136745.0
52	136745.0
53	136745.0
54	129164.0
55	121583.0
56	121583.0
57	121583.0
58	112407.0
59	103231.0
60	103231.0
61	103231.0
62	90172.0
63	77113.0
64	77113.0
65	77113.0
66	62548.5
67	47984.0
68	47984.0
69	47984.0
70	35984.0
71	23984.0
72	23984.0
73	23984.0
74	18036.0
75	12088.0
76	12088.0
77	12088.0
78	9473.0
79	6858.0
80	6858.0
81	6858.0
82	4715.0
83	2572.0
84	2572.0
85	2572.0
86	1936.0
87	1300.0
88	1300.0
89	1300.0
90	867.0
91	434.0
92	434.0
93	434.0
94	263.5
95	93.0
96	93.0
97	93.0
98	253.5
99	414.0
100	414.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	4.05263152562327E-4
2	0.0
3	6.078947288434904E-4
4	0.010131578814058174
5	0.032623683781267315
6	0.061093420248770784
7	0.10546973545434558
8	0.10921841961554711
9	0.1176276300312154
10	0.13221710352345917
11	0.1403223665747057
12	0.13373684034556788
13	0.12897499830296055
14	0.1159052616328255
15	0.13393947192184905
16	0.11610789320910667
17	0.12340262995522856
18	0.11114341959021816
19	0.09513552506400624
20	0.09158947247908589
21	0.09239999878421054
22	0.10394999863223686
23	0.08166052524130887
24	0.0909815777502424
25	0.08804341989416553
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	987013.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.559961099382775
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.42545484226842	44.75471662565874
2	14.462795580001991	16.938814931064837
3	4.424734064449205	7.773367640677778
4	1.7702980434399207	4.146743382326208
5	0.8803069402516203	2.5775370088325777
6	0.48592799056596075	1.7073554534786337
7	0.29871326559083905	1.224484604900839
8	0.22255559799842592	1.0426277728990152
9	0.14522332200044114	0.7653844878362073
>10	0.7595142059237417	7.953063434738588
>50	0.06529199461178493	2.670207239947631
>100	0.05465830625954512	6.370689743440024
>500	0.004003633564507576	1.6079372692480702
>1k	5.222130736314228E-4	0.4670704049508468
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	2113	0.21408026034104918	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1289	0.13059605091320986	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	1180	0.11955263000588644	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.0263157628116346E-4
2	0.0	0.0	0.0	0.0	2.0263157628116346E-4
3	0.0	0.0	0.0	0.0	2.0263157628116346E-4
4	0.0	0.0	0.0	0.0	2.0263157628116346E-4
5	1.0131578814058173E-4	0.0	0.0	0.0	3.039473644217452E-4
6	1.0131578814058173E-4	0.0	0.0	0.0	3.039473644217452E-4
7	1.0131578814058173E-4	0.0	0.0	0.0	3.039473644217452E-4
8	1.0131578814058173E-4	0.0	0.0	0.0	3.039473644217452E-4
9	1.0131578814058173E-4	0.0	0.0	0.0	3.039473644217452E-4
10	1.0131578814058173E-4	0.0	0.0	1.0131578814058173E-4	3.039473644217452E-4
11	1.0131578814058173E-4	0.0	0.0	1.0131578814058173E-4	3.039473644217452E-4
12	1.0131578814058173E-4	0.0	0.0	1.0131578814058173E-4	0.0012157894576869809
13	1.0131578814058173E-4	0.0	0.0	1.0131578814058173E-4	0.0014184210339681443
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACCGCG	40	0.005271775	14.252246	7
CCAACCG	55	1.9555484E-4	13.82106	9
CGTTATT	80	2.0017214E-6	13.05992	2
TTCGAAG	80	2.0017214E-6	13.05992	2
GCGTTAT	80	2.0074294E-6	13.056608	1
TTTTCGG	95	7.385279E-8	12.99875	16
CGAGCCG	185	0.0	12.837907	15
ATCGAAC	60	4.082485E-4	12.669306	8
CGAACCC	60	4.084233E-4	12.668663	10
CGAAGAC	75	1.4772026E-5	12.666734	4
AAGACGG	185	0.0	12.325642	5
TCTAGCC	70	1.0900652E-4	12.214351	3
GTATAGA	70	1.09472116E-4	12.208776	1
GCGGTCG	95	1.0358708E-6	12.0025	9
TAGAACC	95	1.0384138E-6	12.000064	4
CGAGGTT	95	1.0384138E-6	12.000064	4
CCGAGGT	120	9.991709E-9	11.875063	3
GTATTAG	105	2.731722E-7	11.756598	1
AGACGAT	65	8.008259E-4	11.69415	7
AATCGAA	65	8.008259E-4	11.69415	7
>>END_MODULE