FastQCFastQC Report
Thu 2 Feb 2017
SRR4062319_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062319_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences987013
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT10560.10698947227645432No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGATCG407.032206E-427.49933431
GTATCAA13500.024.7760491
TAGGACG450.001398228824.4438534
TACCGTC2000.020.8994947
GGTATCA6950.020.8968751
CGCAATA1800.020.7783336
GTATTAT1201.4268153E-820.1712881
CTAGCGG1900.019.68373529
ATACCGT2250.019.5550826
ACCGTCG2150.019.4413918
GTATATG1605.2750693E-1119.2544121
GTATTAC600.007401372318.3375341
CGGATCG600.00741219518.3328926
ATACCGA600.00741219518.332896
ATACGAA2050.018.2453140
TAGCGGC2050.018.24346230
GTAGGAC1456.3046173E-918.2064573
CGTCGTA2300.018.17347510
TAGGACA1601.1004886E-917.8745674
TCTAGCG2100.017.80909328
Produced by FastQC (version 0.11.5)