##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062319_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	987013
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.247553983584815	32.0	32.0	32.0	32.0	32.0
2	31.447142033590236	32.0	32.0	32.0	32.0	32.0
3	31.561493111032984	32.0	32.0	32.0	32.0	32.0
4	31.641921636290505	32.0	32.0	32.0	32.0	32.0
5	31.6285124917301	32.0	32.0	32.0	32.0	32.0
6	35.26625586491769	36.0	36.0	36.0	36.0	36.0
7	35.256427220310165	36.0	36.0	36.0	36.0	36.0
8	35.21758274713707	36.0	36.0	36.0	36.0	36.0
9	35.28775304884535	36.0	36.0	36.0	36.0	36.0
10	35.18250418180916	36.0	36.0	36.0	36.0	36.0
11	35.29067702249109	36.0	36.0	36.0	36.0	36.0
12	35.20960108934735	36.0	36.0	36.0	36.0	36.0
13	35.25281936509448	36.0	36.0	36.0	36.0	36.0
14	35.21375503666112	36.0	36.0	36.0	36.0	36.0
15	35.1952902342725	36.0	36.0	36.0	36.0	36.0
16	35.200575878939794	36.0	36.0	36.0	36.0	36.0
17	35.186070497551704	36.0	36.0	36.0	36.0	36.0
18	35.181645023925725	36.0	36.0	36.0	36.0	36.0
19	35.1781861029186	36.0	36.0	36.0	36.0	36.0
20	35.169913668816925	36.0	36.0	36.0	36.0	36.0
21	35.16021673473399	36.0	36.0	36.0	36.0	36.0
22	35.14987036644907	36.0	36.0	36.0	36.0	36.0
23	35.12556065624262	36.0	36.0	36.0	36.0	36.0
24	35.10691551175111	36.0	36.0	36.0	36.0	36.0
25	35.09643743294161	36.0	36.0	36.0	36.0	36.0
26	35.050717670385296	36.0	36.0	36.0	36.0	36.0
27	35.04086876262015	36.0	36.0	36.0	36.0	36.0
28	35.022371539179325	36.0	36.0	36.0	36.0	36.0
29	35.00099390788166	36.0	36.0	36.0	36.0	36.0
30	34.98118869761594	36.0	36.0	36.0	36.0	36.0
31	34.984903947567055	36.0	36.0	36.0	36.0	36.0
32	34.96244831628358	36.0	36.0	36.0	36.0	36.0
33	34.933726303503605	36.0	36.0	36.0	36.0	36.0
34	34.916177395839775	36.0	36.0	36.0	36.0	36.0
35	34.90749463279612	36.0	36.0	36.0	36.0	36.0
36	34.892959869829475	36.0	36.0	36.0	32.0	36.0
37	34.87322355429969	36.0	36.0	36.0	32.0	36.0
38	34.86750934384856	36.0	36.0	36.0	32.0	36.0
39	34.84779025200276	36.0	36.0	36.0	32.0	36.0
40	34.83293431798771	36.0	36.0	36.0	32.0	36.0
41	34.80135013419276	36.0	36.0	36.0	32.0	36.0
42	34.77419041086591	36.0	36.0	36.0	32.0	36.0
43	34.75945909527027	36.0	36.0	36.0	32.0	36.0
44	34.7208152273577	36.0	36.0	36.0	32.0	36.0
45	34.69443158296801	36.0	36.0	36.0	32.0	36.0
46	34.67968709631991	36.0	36.0	36.0	32.0	36.0
47	34.64386082047552	36.0	36.0	36.0	32.0	36.0
48	34.62034643920597	36.0	36.0	36.0	32.0	36.0
49	34.60145813682292	36.0	36.0	36.0	32.0	36.0
50	34.18971280013536	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	3.0
20	16.0
21	45.0
22	92.0
23	236.0
24	559.0
25	1313.0
26	2585.0
27	4867.0
28	8245.0
29	12738.0
30	18821.0
31	27133.0
32	40557.0
33	67743.0
34	154090.0
35	647969.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.36682382224528	17.60277609992014	11.613972928161118	26.41642714967346
2	16.458893547347753	19.88887245256714	36.64939501952432	27.002838980560785
3	19.07538930709922	23.078793527927782	28.177526063565715	29.66829110140728
4	12.889495883032948	15.631607689057795	34.847666646741224	36.63122978116803
5	14.648439280941588	36.51714820372173	33.154071932183264	15.680340583153413
6	35.30109056424846	34.48580159106475	16.16748327274643	14.045624571940362
7	30.839715383687956	30.16252065575631	19.813112897195882	19.184651063359855
8	28.41634304715338	32.984975881776634	18.834605015334144	19.764076055735842
9	26.933079908775266	14.416122178735236	18.564395808363212	40.08640210412629
10	16.57436789015736	26.712745133797767	30.919988814725656	25.792898161319215
11	37.59545213690195	21.28634577254808	21.43061945486027	19.6875826356897
12	24.64536941256093	23.63747995213842	28.230631207491697	23.486519427808954
13	29.585831189660116	19.34756685068991	24.846683883596267	26.219918076053712
14	23.637682583714703	19.34057606130821	24.935335198219274	32.08640615675781
15	25.28771150937222	26.73196806931621	22.12382207731813	25.856498343993444
16	25.952121871599566	25.757999444785547	23.420526318988944	24.86935236462595
17	24.196844418462575	25.955686500583074	24.942528619177253	24.9049404617771
18	24.939919676761782	24.945998630209157	25.80100343258238	24.313078260446684
19	25.572712821411674	24.905852303870365	25.376666771359645	24.144768103358313
20	25.831908150693817	24.287239819738037	24.975785403968356	24.905066625599794
21	26.51454438796652	24.248312838837986	24.714162832708382	24.522979940487104
22	26.01070097354341	24.19633783952187	25.05812993344566	24.734831253489062
23	24.572345624977203	24.289269026111132	25.628867734130896	25.509517614780776
24	24.79617589814823	24.79060347334304	25.575707469987425	24.8375131585213
25	24.990856250120313	24.244361523100505	25.494902296119708	25.26987993065947
26	24.592127139413847	25.1968823042499	25.559796192747598	24.65119436358865
27	25.053596106227683	24.689669426511532	25.168893589946222	25.08784087731456
28	24.89336394455128	24.42446925132877	25.64939078137633	25.03277602274362
29	24.680879949666217	24.83832500347007	25.612277877348884	24.868517169514828
30	24.681283921998524	24.802964923247757	25.726565262455082	24.789185892298637
31	25.088043509096142	24.665961508066772	25.096351412140887	25.1496435706962
32	25.0130444077231	24.673636517452152	24.975354934534806	25.337964140289948
33	24.485619222461327	24.514696883117935	25.46524257050573	25.534441323915008
34	25.25093591217876	24.59764641516507	25.38001327247582	24.771404400180344
35	25.428389349257507	24.331238456309	25.660099026251988	24.58027316818151
36	24.620774620774622	24.828776144565616	25.40029908450961	25.150150150150154
37	25.381965552178322	24.76231003039514	25.152887537993923	24.702836879432624
38	24.68559178045274	24.589341781719188	25.49692861188252	25.228137825945556
39	25.326365466506317	24.583209642890072	25.027178125383102	25.06324676522051
40	25.49259786101891	24.614999614999615	25.275883170620013	24.616519353361458
41	24.52533120433149	25.19949260590636	25.64457692167408	24.630599268088073
42	25.59158393019436	25.01527403596263	25.15813566586185	24.235006367981164
43	25.286508492161353	24.37128217806233	25.28914282008857	25.053066509687742
44	24.624710731408793	24.9694013609302	25.2197630733192	25.186124834341804
45	24.89508548177598	25.051824023246343	25.145745566842354	24.907344928135327
46	24.713142699665347	24.852556614106135	25.189743756009435	25.24455693021908
47	24.816438517161618	24.810967386998367	25.445213217034667	24.92738087880535
48	25.445126331478157	25.28686369453082	24.5755937128597	24.692416261131324
49	24.796979027667593	25.387474961168916	24.693834748274256	25.121711262889235
50	25.062284638009487	25.355394776702354	24.86299405978312	24.71932652550504
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	256.0
1	163.0
2	70.0
3	152.0
4	234.0
5	263.0
6	292.0
7	219.0
8	146.0
9	216.5
10	287.0
11	492.0
12	697.0
13	1192.5
14	1688.0
15	2215.0
16	2742.0
17	2669.5
18	2597.0
19	2550.0
20	2503.0
21	2780.0
22	3057.0
23	3500.0
24	3943.0
25	4722.0
26	5501.0
27	6751.5
28	8002.0
29	9587.0
30	11172.0
31	12722.5
32	14273.0
33	16690.5
34	19108.0
35	21579.0
36	24050.0
37	28535.0
38	33020.0
39	36857.0
40	40694.0
41	44511.5
42	48329.0
43	51167.5
44	54006.0
45	59252.5
46	64499.0
47	68344.0
48	72189.0
49	75797.0
50	79405.0
51	77755.0
52	76105.0
53	75924.0
54	75743.0
55	75512.0
56	75281.0
57	72334.5
58	69388.0
59	63900.0
60	58412.0
61	51147.0
62	43882.0
63	37528.0
64	31174.0
65	26380.0
66	21586.0
67	18195.0
68	14804.0
69	12895.5
70	10987.0
71	8638.0
72	6289.0
73	5359.5
74	4430.0
75	3228.0
76	2026.0
77	1772.5
78	1519.0
79	1328.0
80	1137.0
81	905.5
82	674.0
83	560.5
84	447.0
85	334.5
86	222.0
87	153.5
88	85.0
89	54.0
90	23.0
91	16.5
92	10.0
93	10.5
94	11.0
95	8.0
96	5.0
97	7.5
98	10.0
99	6.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03090131538287743
2	0.003951315737482687
3	3.039473644217452E-4
4	0.0
5	0.0
6	1.0131578814058174E-4
7	0.0
8	0.0
9	0.0
10	1.0131578814058174E-4
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	7.092105169840721E-4
17	0.0
18	1.0131578814058174E-4
19	0.0
20	5.065789407029086E-4
21	0.0
22	0.0
23	1.0131578814058174E-4
24	0.0010131578814058172
25	0.0
26	2.026315762811635E-4
27	1.0131578814058174E-4
28	0.0011144736695463992
29	2.026315762811635E-4
30	6.078947288434904E-4
31	1.0131578814058174E-4
32	0.0
33	1.0131578814058174E-4
34	8.10526305124654E-4
35	2.026315762811635E-4
36	1.0131578814058174E-4
37	0.0013171052458275626
38	0.0
39	6.078947288434904E-4
40	1.0131578814058174E-4
41	9.118420932652355E-4
42	0.004457894678185597
43	0.004457894678185597
44	0.0041539473137638516
45	0.0019249999746710528
46	0.0024315789153739617
47	0.0014184210339681443
48	0.00466052625446676
49	0.00466052625446676
50	0.0012157894576869809
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	987013.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.68156851406315
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.9931592177172	45.35390938452299
2	14.906972895755585	17.793430484306388
3	4.494992257880556	8.048045652266456
4	1.8002697284968057	4.2977168458030395
5	0.8495910817953805	2.5352464178554013
6	0.4820419636219787	1.7261412287135198
7	0.3044590810086158	1.2719416852061085
8	0.20298572540759338	0.9691605182632075
9	0.1478519722772705	0.7941633852064758
>10	0.7055187419328269	7.529611578666937
>50	0.059164079702450995	2.422393725308578
>100	0.04940338925185132	5.703609115006547
>500	0.0034189191922720874	1.4468934479850288
>1k	1.7094595961360438E-4	0.1077365308893097
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	1056	0.10698947227645432	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.039473644217452E-4
2	0.0	0.0	0.0	0.0	3.039473644217452E-4
3	0.0	0.0	0.0	0.0	3.039473644217452E-4
4	0.0	0.0	0.0	0.0	3.039473644217452E-4
5	0.0	1.0131578814058173E-4	0.0	0.0	3.039473644217452E-4
6	0.0	1.0131578814058173E-4	0.0	0.0	4.052631525623269E-4
7	0.0	1.0131578814058173E-4	0.0	0.0	4.052631525623269E-4
8	0.0	1.0131578814058173E-4	0.0	0.0	4.052631525623269E-4
9	0.0	1.0131578814058173E-4	0.0	0.0	4.052631525623269E-4
10	0.0	1.0131578814058173E-4	0.0	1.0131578814058173E-4	4.052631525623269E-4
11	0.0	1.0131578814058173E-4	0.0	1.0131578814058173E-4	4.052631525623269E-4
12	0.0	1.0131578814058173E-4	0.0	1.0131578814058173E-4	9.118420932652357E-4
13	0.0	1.0131578814058173E-4	0.0	1.0131578814058173E-4	9.118420932652357E-4
14	0.0	1.0131578814058173E-4	0.0	1.0131578814058173E-4	9.118420932652357E-4
15	0.0	1.0131578814058173E-4	0.0	1.0131578814058173E-4	0.0011144736695463992
16	0.0	1.0131578814058173E-4	0.0	2.0263157628116346E-4	0.0011144736695463992
17	0.0	1.0131578814058173E-4	0.0	2.0263157628116346E-4	0.0011144736695463992
18	0.0	1.0131578814058173E-4	0.0	3.039473644217452E-4	0.0011144736695463992
19	0.0	1.0131578814058173E-4	0.0	6.078947288434904E-4	0.0011144736695463992
20	0.0	2.0263157628116346E-4	0.0	6.078947288434904E-4	0.0016210526102493077
21	0.0	2.0263157628116346E-4	0.0	6.078947288434904E-4	0.0016210526102493077
22	0.0	2.0263157628116346E-4	0.0	7.092105169840721E-4	0.0016210526102493077
23	0.0	2.0263157628116346E-4	0.0	0.0013171052458275626	0.0016210526102493077
24	0.0	2.0263157628116346E-4	0.0	0.001924999974671053	0.0016210526102493077
25	0.0	2.0263157628116346E-4	0.0	0.00233026312723338	0.0016210526102493077
26	0.0	2.0263157628116346E-4	0.0	0.003039473644217452	0.0017223683983898896
27	0.0	2.0263157628116346E-4	0.0	0.003444736796779779	0.0017223683983898896
28	0.0	2.0263157628116346E-4	0.0	0.005471052559591414	0.0017223683983898896
29	0.0	2.0263157628116346E-4	0.0	0.0105368419666205	0.0017223683983898896
30	0.0	2.0263157628116346E-4	0.0	0.01641315767877424	0.0017223683983898896
31	0.0	2.0263157628116346E-4	0.0	0.03130657853543976	0.0017223683983898896
32	0.0	2.0263157628116346E-4	0.0	0.04731447306165167	0.0017223683983898896
33	0.0	2.0263157628116346E-4	0.0	0.06180263076575486	0.0017223683983898896
34	0.0	2.0263157628116346E-4	0.0	0.07649342004613921	0.0020263157628116345
35	0.0	2.0263157628116346E-4	0.0	0.09391973560631926	0.0020263157628116345
36	0.0	2.0263157628116346E-4	0.0	0.11965394579402702	0.0020263157628116345
37	0.0	2.0263157628116346E-4	0.0	0.15460789270252773	0.0020263157628116345
38	0.0	2.0263157628116346E-4	0.0	0.19999736578950836	0.0020263157628116345
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGGATCG	40	7.032206E-4	27.499334	31
GTATCAA	1350	0.0	24.776049	1
TAGGACG	45	0.0013982288	24.443853	4
TACCGTC	200	0.0	20.899494	7
GGTATCA	695	0.0	20.896875	1
CGCAATA	180	0.0	20.77833	36
GTATTAT	120	1.4268153E-8	20.171288	1
CTAGCGG	190	0.0	19.683735	29
ATACCGT	225	0.0	19.555082	6
ACCGTCG	215	0.0	19.441391	8
GTATATG	160	5.2750693E-11	19.254412	1
GTATTAC	60	0.0074013723	18.337534	1
CGGATCG	60	0.007412195	18.33289	26
ATACCGA	60	0.007412195	18.33289	6
ATACGAA	205	0.0	18.24531	40
TAGCGGC	205	0.0	18.243462	30
GTAGGAC	145	6.3046173E-9	18.206457	3
CGTCGTA	230	0.0	18.173475	10
TAGGACA	160	1.1004886E-9	17.874567	4
TCTAGCG	210	0.0	17.809093	28
>>END_MODULE