FastQCFastQC Report
Thu 2 Feb 2017
SRR4062318_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062318_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2070227
Sequences flagged as poor quality0
Sequence length25
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT32590.15742235030264798No Hit
GCGCAAGACGGACCAGAGCGAAAGC26290.1269909048621238No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTAAC1950.013.1553711
GTATCAA22700.012.9703651
GGTATCA8350.012.7393691
GCGTTAT1501.8189894E-1212.663541
CGACCAT3250.012.57038210
TCGCGTA2050.012.513049
CGTTATT1600.012.4671782
CGTCGTA2100.012.215404510
CGCGTAA2100.012.215404510
CCGACCA3500.012.215119
TACGCTG701.09104185E-412.21451955
CAAGACG5100.012.1077814
AATCGGA550.003069152812.09084716
CGCATCG2450.012.02092813
GCGCAAG5200.011.8720681
TAGGACG658.0257293E-411.6922484
AAGACGG5350.011.5422765
CGTGCGC1001.928278E-611.40104410
ACGGTAT2500.011.4007699
GGGTAGG5450.011.3274781
Produced by FastQC (version 0.11.5)