##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062318_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2070227
Sequences flagged as poor quality	0
Sequence length	25
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.204000817301676	32.0	32.0	32.0	32.0	32.0
2	30.747105510651732	32.0	32.0	32.0	32.0	32.0
3	30.768906984596374	32.0	32.0	32.0	32.0	32.0
4	30.78104719917188	32.0	32.0	32.0	32.0	32.0
5	30.698520983447708	32.0	32.0	32.0	32.0	32.0
6	34.32943730325225	36.0	36.0	36.0	32.0	36.0
7	34.26201377916528	36.0	36.0	36.0	32.0	36.0
8	34.22579794389697	36.0	36.0	36.0	32.0	36.0
9	34.349171371062205	36.0	36.0	36.0	32.0	36.0
10	34.06041801213104	36.0	36.0	36.0	32.0	36.0
11	34.32557154360367	36.0	36.0	36.0	32.0	36.0
12	34.15806382585098	36.0	36.0	36.0	32.0	36.0
13	34.23750052530471	36.0	36.0	36.0	32.0	36.0
14	34.14708966697855	36.0	36.0	36.0	32.0	36.0
15	34.10667429223945	36.0	36.0	36.0	32.0	36.0
16	34.10716457663821	36.0	36.0	36.0	32.0	36.0
17	34.038822312722225	36.0	36.0	36.0	32.0	36.0
18	34.03559126607855	36.0	36.0	36.0	32.0	36.0
19	34.04945013276322	36.0	36.0	36.0	32.0	36.0
20	34.035603342048965	36.0	36.0	36.0	32.0	36.0
21	34.019373237814015	36.0	36.0	36.0	32.0	36.0
22	33.987643867073515	36.0	36.0	36.0	32.0	36.0
23	33.95571162003007	36.0	36.0	36.0	32.0	36.0
24	33.93748994675463	36.0	36.0	36.0	32.0	36.0
25	33.5783245025787	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	1.0
4	36.0
5	160.0
6	411.0
7	101.0
8	344.0
9	269.0
10	164.0
11	46.0
12	76.0
13	64.0
14	252.0
15	375.0
16	643.0
17	887.0
18	1397.0
19	1846.0
20	2830.0
21	4206.0
22	6523.0
23	9398.0
24	13522.0
25	19096.0
26	25609.0
27	32926.0
28	43309.0
29	57378.0
30	74186.0
31	99644.0
32	140575.0
33	196379.0
34	424927.0
35	912647.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.67996889196964	16.927502348801205	11.459396533193573	25.93313222603559
2	16.874494318250434	18.83804900433528	37.1231629412262	27.164293736188093
3	19.75268328969868	22.034131638183382	27.69362676430524	30.519558307812694
4	12.876652769793381	15.101134777126681	34.82393804860846	37.198274404471476
5	15.180666886684735	36.05166784487469	33.22179415876188	15.545871109678686
6	35.779764189108846	34.46315176906502	16.031109560133515	13.72597448169261
7	31.38564257644677	29.807458440780376	19.503373430241584	19.30352555253127
8	28.585741654650448	33.08522377248387	18.466053331708604	19.862981241157076
9	26.741025205784453	14.597264073886546	18.345464472470752	40.31624624785825
10	16.254176493235388	26.61284705763996	30.85062132289637	26.28235512622828
11	37.619170616044975	21.267390082489527	21.4669147060047	19.6465245954608
12	24.513501617872535	23.607154430649132	28.19185211339134	23.687491838086995
13	29.328965072290075	19.337535221281055	24.96399279952719	26.36950690690168
14	23.466910134392442	19.351477030551298	24.651977683629617	32.52963515142664
15	25.23242709900219	27.01542420182537	21.69661383389842	26.055534865274023
16	26.073842445720295	26.00981329020876	22.987579117596994	24.928765146473946
17	24.157900031339352	26.158975628818276	24.628087022800347	25.05503731704203
18	24.970538783630154	25.375379659212182	25.30758380307673	24.34649775408093
19	25.565655818433147	25.158260820795036	25.023993563681213	24.2520897970906
20	25.687863149680762	24.635339461854354	24.592697023054956	25.084100365409924
21	26.442249815435538	24.32489534094991	24.416561149458296	24.816293694156254
22	25.831097426236827	24.306151569116004	24.94953192217738	24.913219082469794
23	24.586604067102453	24.510562226203447	25.29544164695122	25.607392059742878
24	24.680342701825143	25.023798737857085	24.99735300985744	25.29850555046033
25	24.857430165437087	24.639490248784117	24.99666418494919	25.506415400829603
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	216.0
1	216.0
2	365.5
3	515.0
4	515.0
5	515.0
6	1184.0
7	1853.0
8	1853.0
9	1853.0
10	2363.0
11	2873.0
12	2873.0
13	2873.0
14	4062.0
15	5251.0
16	5251.0
17	5251.0
18	8822.5
19	12394.0
20	12394.0
21	12394.0
22	19069.5
23	25745.0
24	25745.0
25	25745.0
26	38005.0
27	50265.0
28	50265.0
29	50265.0
30	63651.0
31	77037.0
32	77037.0
33	77037.0
34	96601.0
35	116165.0
36	116165.0
37	116165.0
38	139032.5
39	161900.0
40	161900.0
41	161900.0
42	188787.0
43	215674.0
44	215674.0
45	215674.0
46	241682.0
47	267690.0
48	267690.0
49	267690.0
50	278440.5
51	289191.0
52	289191.0
53	289191.0
54	272307.0
55	255423.0
56	255423.0
57	255423.0
58	236424.5
59	217426.0
60	217426.0
61	217426.0
62	191247.0
63	165068.0
64	165068.0
65	165068.0
66	134105.0
67	103142.0
68	103142.0
69	103142.0
70	77740.0
71	52338.0
72	52338.0
73	52338.0
74	39014.5
75	25691.0
76	25691.0
77	25691.0
78	20139.5
79	14588.0
80	14588.0
81	14588.0
82	9982.0
83	5376.0
84	5376.0
85	5376.0
86	3951.5
87	2527.0
88	2527.0
89	2527.0
90	1658.0
91	789.0
92	789.0
93	789.0
94	502.5
95	216.0
96	216.0
97	216.0
98	545.0
99	874.0
100	874.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0010626853963357642
2	9.66077633032513E-5
3	3.381271715613795E-4
4	0.010481942318402764
5	0.03381271715613795
6	0.06187727239573245
7	0.10404656107760163
8	0.10573719693540853
9	0.11631574701711454
10	0.13056539210434412
11	0.13587881908602295
12	0.13027556881443436
13	0.12486553406945229
14	0.11245143648498451
15	0.13027556881443436
16	0.11684708971528243
17	0.12264355551347751
18	0.1088769492427642
19	0.09486882356379277
20	0.09008673928028182
21	0.08907235776559769
22	0.10085850488859435
23	0.07868702321049817
24	0.08887914223899118
25	0.08535295887842252
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2070227.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.72884860040193
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.99129432216152	38.79213310339275
2	14.289023508351193	14.783094674221658
3	4.64920831645083	7.214945793402437
4	2.0989271501831954	4.343003391003984
5	1.0463725761028086	2.706382428441737
6	0.6522433053444358	2.024387711567283
7	0.42711062895300345	1.5465758742512654
8	0.2776109762200199	1.1488396926956153
9	0.23055375674300893	1.0733652339131703
>10	1.1698553838400483	10.86235803508924
>50	0.08564019782009609	3.076169036002903
>100	0.07196227670684004	7.59912329435629
>500	0.007110253994147439	2.48631511061797
>1k	0.0030873471290377014	2.343306621043738
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	3259	0.15742235030264798	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	2629	0.1269909048621238	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.830388165162564E-5
2	0.0	0.0	0.0	0.0	4.830388165162564E-5
3	0.0	0.0	0.0	4.830388165162564E-5	4.830388165162564E-5
4	0.0	0.0	0.0	4.830388165162564E-5	4.830388165162564E-5
5	0.0	0.0	0.0	4.830388165162564E-5	9.660776330325129E-5
6	0.0	0.0	0.0	4.830388165162564E-5	1.9321552660650257E-4
7	0.0	0.0	0.0	4.830388165162564E-5	1.9321552660650257E-4
8	0.0	0.0	0.0	4.830388165162564E-5	1.9321552660650257E-4
9	0.0	0.0	0.0	4.830388165162564E-5	1.9321552660650257E-4
10	0.0	0.0	0.0	4.830388165162564E-5	2.4151940825812822E-4
11	0.0	0.0	0.0	4.830388165162564E-5	2.4151940825812822E-4
12	0.0	0.0	0.0	4.830388165162564E-5	4.347349348646308E-4
13	0.0	0.0	0.0	4.830388165162564E-5	4.347349348646308E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGTAAC	195	0.0	13.15537	11
GTATCAA	2270	0.0	12.970365	1
GGTATCA	835	0.0	12.739369	1
GCGTTAT	150	1.8189894E-12	12.66354	1
CGACCAT	325	0.0	12.570382	10
TCGCGTA	205	0.0	12.51304	9
CGTTATT	160	0.0	12.467178	2
CGTCGTA	210	0.0	12.2154045	10
CGCGTAA	210	0.0	12.2154045	10
CCGACCA	350	0.0	12.21511	9
TACGCTG	70	1.09104185E-4	12.2145195	5
CAAGACG	510	0.0	12.107781	4
AATCGGA	55	0.0030691528	12.090847	16
CGCATCG	245	0.0	12.020928	13
GCGCAAG	520	0.0	11.872068	1
TAGGACG	65	8.0257293E-4	11.692248	4
AAGACGG	535	0.0	11.542276	5
CGTGCGC	100	1.928278E-6	11.401044	10
ACGGTAT	250	0.0	11.400769	9
GGGTAGG	545	0.0	11.327478	1
>>END_MODULE