FastQCFastQC Report
Thu 2 Feb 2017
SRR4062318_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062318_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2070227
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT24470.11819959840152795No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA22850.027.4514881
TAGGACG952.6893758E-823.156554
GGTATCA11100.022.2076341
TTAACGG956.870232E-720.84240335
CGGATCG2400.020.16646826
ATCGATT2450.019.75490829
TGTACGC808.991528E-519.2502752
CGCTTCG4000.019.24934432
TCAACGC32700.019.1059234
ATCAACG32600.019.0970483
TCGATTT2650.019.09415230
CAACGCA33400.018.7713625
TATCAAC34600.018.3759842
CAAGACG8750.018.3532184
CGCGGGA4200.018.3344844
TAGCGTT600.00741633118.3322669
GGATCGA2650.018.26397127
AACGCAG34450.018.2630886
TAACGGC1102.7979986E-618.00025636
CGGACCA7950.017.9863769
Produced by FastQC (version 0.11.5)