##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062318_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2070227
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.26013813944075	32.0	32.0	32.0	32.0	32.0
2	31.395476921129905	32.0	32.0	32.0	32.0	32.0
3	31.535992912854486	32.0	32.0	32.0	32.0	32.0
4	31.618395470641627	32.0	32.0	32.0	32.0	32.0
5	31.592410397507134	32.0	32.0	32.0	32.0	32.0
6	35.21300900819089	36.0	36.0	36.0	36.0	36.0
7	35.21508172775256	36.0	36.0	36.0	36.0	36.0
8	35.16417426687991	36.0	36.0	36.0	36.0	36.0
9	35.25460879410809	36.0	36.0	36.0	36.0	36.0
10	35.13114503868417	36.0	36.0	36.0	36.0	36.0
11	35.25746113831961	36.0	36.0	36.0	36.0	36.0
12	35.17418283115813	36.0	36.0	36.0	36.0	36.0
13	35.20903214961451	36.0	36.0	36.0	36.0	36.0
14	35.1765246033406	36.0	36.0	36.0	36.0	36.0
15	35.15015648042461	36.0	36.0	36.0	36.0	36.0
16	35.166315577953526	36.0	36.0	36.0	36.0	36.0
17	35.14406101359899	36.0	36.0	36.0	36.0	36.0
18	35.14127243051124	36.0	36.0	36.0	36.0	36.0
19	35.13493254604447	36.0	36.0	36.0	36.0	36.0
20	35.13501514568209	36.0	36.0	36.0	36.0	36.0
21	35.13202465236904	36.0	36.0	36.0	36.0	36.0
22	35.10871223300633	36.0	36.0	36.0	36.0	36.0
23	35.079642473989566	36.0	36.0	36.0	36.0	36.0
24	35.063992499373256	36.0	36.0	36.0	36.0	36.0
25	35.04455743259073	36.0	36.0	36.0	36.0	36.0
26	35.005635130833475	36.0	36.0	36.0	36.0	36.0
27	34.989931538908536	36.0	36.0	36.0	36.0	36.0
28	34.97430571623305	36.0	36.0	36.0	36.0	36.0
29	34.95132949188664	36.0	36.0	36.0	36.0	36.0
30	34.93803964492783	36.0	36.0	36.0	36.0	36.0
31	34.93766142553449	36.0	36.0	36.0	32.0	36.0
32	34.90862499619607	36.0	36.0	36.0	32.0	36.0
33	34.892104585632396	36.0	36.0	36.0	32.0	36.0
34	34.8805787964315	36.0	36.0	36.0	32.0	36.0
35	34.86750776605657	36.0	36.0	36.0	32.0	36.0
36	34.84813839255309	36.0	36.0	36.0	32.0	36.0
37	34.83647203905659	36.0	36.0	36.0	32.0	36.0
38	34.81931546637156	36.0	36.0	36.0	32.0	36.0
39	34.81865611838702	36.0	36.0	36.0	32.0	36.0
40	34.80300324553781	36.0	36.0	36.0	32.0	36.0
41	34.76895045808986	36.0	36.0	36.0	32.0	36.0
42	34.7462389390149	36.0	36.0	36.0	32.0	36.0
43	34.743508320585136	36.0	36.0	36.0	32.0	36.0
44	34.69664630980081	36.0	36.0	36.0	32.0	36.0
45	34.683959778323825	36.0	36.0	36.0	32.0	36.0
46	34.66998594840083	36.0	36.0	36.0	32.0	36.0
47	34.64483218506956	36.0	36.0	36.0	32.0	36.0
48	34.61967069311723	36.0	36.0	36.0	32.0	36.0
49	34.612574369863786	36.0	36.0	36.0	32.0	36.0
50	34.18675053508625	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	6.0
20	11.0
21	47.0
22	113.0
23	396.0
24	956.0
25	2255.0
26	5050.0
27	9382.0
28	16426.0
29	26803.0
30	40207.0
31	60214.0
32	91470.0
33	155412.0
34	350954.0
35	1310524.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.374065331938525	17.42904978534344	11.860252176160921	26.33663270655711
2	16.303848489254577	19.34702872321977	36.834422069650465	27.514700717875197
3	18.987411476756808	22.555729611215046	28.03732358750451	30.41953532452364
4	12.67094156432272	15.497494233718555	34.97571520003864	36.855849001920085
5	14.825572268161896	36.42209284295877	32.99956961241449	15.75276527646485
6	35.92579156931403	34.62284014813863	15.916352972602468	13.53501530994487
7	31.477610909335063	29.776396501446463	19.47646320910702	19.269529380111454
8	28.592275146638507	33.19360630500906	18.37436184534353	19.8397567030089
9	27.035392737124965	14.282733246160928	18.181146318737028	40.50072769797708
10	16.218575275634294	26.684056080860774	30.905819415763986	26.191549227740946
11	37.937965256950086	21.106091264387917	21.44639211062362	19.509551368038384
12	24.574068447566376	23.416224404376912	28.19951628492914	23.81019086312757
13	29.496572115038592	19.13790130261078	24.955475897087613	26.410050685263016
14	23.573163715863043	19.124327911866672	24.654301194989728	32.64820717728056
15	25.337897728123533	26.87106293174613	21.74428214876919	26.04675719136114
16	26.167826128120087	25.92099174288262	23.02206438561424	24.889117743383053
17	24.388195110970923	25.975798789214906	24.596529752534384	25.039476347279795
18	25.163521031309187	25.2148198527405	25.32504952365035	24.29660959229996
19	25.817217145752615	24.996196069319936	24.9282325078361	24.258354277091353
20	25.856563139877338	24.44565097156294	24.600803489096094	25.09698239946363
21	26.64640157818442	24.19372368344148	24.39379836124251	24.76607637713159
22	25.95541455115792	24.19468976107451	24.880749792172548	24.969145895595023
23	24.722035178767925	24.32579824618182	25.30670564469773	25.64546093035253
24	24.83077795526925	24.838168513420626	25.060126779470092	25.270926751840033
25	25.059293157931855	24.36782142205039	25.131797459402904	25.44108796061485
26	24.953833386709327	25.209458516224615	25.205642494827842	24.631065602238216
27	25.453672467027594	24.839147219420582	24.70727692182845	24.99990339172338
28	25.079388848366573	24.4820523720045	25.326515923659183	25.11204285596974
29	24.877742462153783	24.945706253441664	25.230168774333162	24.946382510071395
30	24.872681321940547	24.988805424019624	25.39379064524715	24.74472260879268
31	25.34490312377579	24.74786483942037	24.580636154918874	25.326595881884963
32	25.308387035692565	24.703664899210562	24.486150418774546	25.501797646322323
33	24.840765869554087	24.6996215864772	24.90138738743961	25.558225156529108
34	25.387799384312775	24.60821550695172	25.248876078575716	24.755109030159787
35	25.892163203831863	24.399568934910814	25.180741573001143	24.527526288256183
36	24.731888657351725	25.06050075788973	24.87293633243198	25.33467425232656
37	25.774265055026373	24.684944224899272	24.74382773746745	24.796962982606903
38	25.015179494809026	24.457801004430916	25.18052368170254	25.346495819057523
39	25.642059847010838	24.59569358114048	24.528985437240244	25.233261134608437
40	25.753335990049393	24.647369247304038	24.961296477436026	24.637998285210543
41	24.683932267577365	25.081670655804615	25.24363529215706	24.99076178446096
42	25.88603045602889	24.921357671877928	24.88855783109483	24.304054040998352
43	25.458458223940834	24.16115779329467	25.023078093563495	25.357305889200997
44	24.89221769489577	24.8915414137657	24.92699786729915	25.289243024039376
45	25.223085004890887	25.04860700182352	24.741676428321277	24.986631564964316
46	25.018778623815496	24.9098505294002	24.859033520386944	25.21233732639736
47	25.142631077851863	24.79725398644866	25.1114263784048	24.94868855729468
48	25.70160476526546	25.308299362315818	24.274797876672526	24.715297995746198
49	25.104013749668503	25.259170913924965	24.50116150498585	25.13565383142068
50	25.292749209980474	25.26115808988688	24.435731392395525	25.010361307737117
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	83.0
1	64.5
2	46.0
3	118.5
4	191.0
5	215.0
6	239.0
7	203.5
8	168.0
9	249.0
10	330.0
11	501.0
12	672.0
13	1264.0
14	1856.0
15	2475.0
16	3094.0
17	3168.0
18	3242.0
19	3296.5
20	3351.0
21	4177.0
22	5003.0
23	6178.5
24	7354.0
25	9421.5
26	11489.0
27	14457.5
28	17426.0
29	20222.0
30	23018.0
31	26095.5
32	29173.0
33	33881.5
34	38590.0
35	44432.5
36	50275.0
37	59568.0
38	68861.0
39	76752.5
40	84644.0
41	93083.0
42	101522.0
43	107318.5
44	113115.0
45	123456.5
46	133798.0
47	143183.5
48	152569.0
49	159472.5
50	166376.0
51	164413.0
52	162450.0
53	161158.5
54	159867.0
55	160817.5
56	161768.0
57	155545.5
58	149323.0
59	136568.0
60	123813.0
61	107879.0
62	91945.0
63	79116.5
64	66288.0
65	55742.0
66	45196.0
67	38385.5
68	31575.0
69	27909.5
70	24244.0
71	18929.5
72	13615.0
73	11742.0
74	9869.0
75	7285.0
76	4701.0
77	4114.5
78	3528.0
79	2957.0
80	2386.0
81	1897.0
82	1408.0
83	1146.5
84	885.0
85	718.0
86	551.0
87	360.0
88	169.0
89	109.0
90	49.0
91	40.5
92	32.0
93	28.0
94	24.0
95	23.0
96	22.0
97	23.0
98	24.0
99	17.0
100	10.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03101109202034366
2	0.004347349348646308
3	3.864310532130052E-4
4	9.66077633032513E-5
5	0.0
6	1.932155266065026E-4
7	0.0
8	0.0
9	0.0
10	9.66077633032513E-5
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	6.279504614711334E-4
17	0.0
18	1.932155266065026E-4
19	0.0
20	2.8982328990975384E-4
21	0.0
22	0.0
23	4.830388165162565E-5
24	8.694698697292616E-4
25	2.415194082581282E-4
26	3.864310532130052E-4
27	5.313426981678821E-4
28	0.0017872436211101488
29	3.381271715613795E-4
30	0.001352508686245518
31	3.864310532130052E-4
32	9.660776330325128E-4
33	2.415194082581282E-4
34	9.660776330325128E-4
35	2.8982328990975384E-4
36	2.415194082581282E-4
37	0.0018355475027617746
38	0.0
39	7.245582247743847E-4
40	9.66077633032513E-5
41	0.0010626853963357642
42	0.004588868756904436
43	0.003960918295433303
44	0.003960918295433303
45	0.0025118018458845336
46	0.0026084096091877848
47	0.0012559009229422668
48	0.003864310532130051
49	0.0032846639523105435
50	0.001207597041290641
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2070227.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.28289133201947
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.14438377293762	38.76482759680045
2	14.899213403193242	15.579479105834404
3	4.845548508076702	7.600178582754098
4	2.136412571089588	4.46791305178549
5	1.0607931737780265	2.7730667115192302
6	0.650105536032627	2.039363825684286
7	0.4063200411092069	1.487051058873404
8	0.3036451284611469	1.2700356203864989
9	0.23865681550125933	1.1229901515448346
>10	1.1580272665263938	10.74970169103097
>50	0.0824772592588356	2.975160882428092
>100	0.06494250633040244	6.83479147250316
>500	0.007059072015421696	2.5232643943066444
>1k	0.002414945689486366	1.8121758545485172
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	2447	0.11819959840152795	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.830388165162564E-5
2	0.0	0.0	0.0	0.0	4.830388165162564E-5
3	0.0	0.0	0.0	0.0	4.830388165162564E-5
4	0.0	0.0	0.0	0.0	4.830388165162564E-5
5	0.0	0.0	0.0	0.0	4.830388165162564E-5
6	0.0	0.0	0.0	0.0	1.9321552660650257E-4
7	0.0	0.0	0.0	0.0	1.9321552660650257E-4
8	0.0	0.0	0.0	0.0	1.9321552660650257E-4
9	0.0	0.0	0.0	0.0	1.9321552660650257E-4
10	0.0	0.0	0.0	4.830388165162564E-5	1.9321552660650257E-4
11	0.0	0.0	0.0	4.830388165162564E-5	1.9321552660650257E-4
12	0.0	0.0	0.0	4.830388165162564E-5	5.313426981678821E-4
13	0.0	0.0	0.0	4.830388165162564E-5	5.313426981678821E-4
14	0.0	0.0	0.0	4.830388165162564E-5	5.796465798195077E-4
15	0.0	0.0	0.0	9.660776330325129E-5	7.245582247743847E-4
16	0.0	0.0	0.0	9.660776330325129E-5	8.694698697292616E-4
17	0.0	0.0	0.0	9.660776330325129E-5	8.694698697292616E-4
18	0.0	0.0	0.0	9.660776330325129E-5	8.694698697292616E-4
19	0.0	0.0	0.0	9.660776330325129E-5	8.694698697292616E-4
20	0.0	0.0	0.0	9.660776330325129E-5	9.660776330325129E-4
21	0.0	0.0	0.0	1.4491164495487692E-4	9.660776330325129E-4
22	0.0	0.0	0.0	2.4151940825812822E-4	9.660776330325129E-4
23	0.0	0.0	0.0	3.381271715613795E-4	0.0010143815146841386
24	0.0	0.0	0.0	4.347349348646308E-4	0.0010143815146841386
25	0.0	0.0	0.0	4.347349348646308E-4	0.0010143815146841386
26	0.0	0.0	0.0	4.8303881651625644E-4	0.001207597041290641
27	0.0	0.0	0.0	4.8303881651625644E-4	0.001207597041290641
28	0.0	0.0	0.0	0.0010626853963357642	0.001207597041290641
29	0.0	0.0	0.0	0.0019804591477166514	0.001207597041290641
30	0.0	0.0	0.0	0.003960918295433303	0.001207597041290641
31	0.0	0.0	0.0	0.006231200733059708	0.0012559009229422668
32	0.0	0.0	0.0	0.009902295738583257	0.001497420331200395
33	0.0	0.0	0.0	0.013138655809242174	0.001497420331200395
34	0.0	0.0	0.0	0.01651992752485597	0.0015457242128520206
35	0.0	0.0	0.0	0.020577453583592524	0.0015457242128520206
36	0.0	0.0	0.0	0.027243389251516863	0.0015457242128520206
37	0.0	0.0	0.0	0.034827098670822086	0.0015457242128520206
38	0.0	0.0	0.0	0.04931826316630978	0.0017872436211101488
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	2285	0.0	27.451488	1
TAGGACG	95	2.6893758E-8	23.15655	4
GGTATCA	1110	0.0	22.207634	1
TTAACGG	95	6.870232E-7	20.842403	35
CGGATCG	240	0.0	20.166468	26
ATCGATT	245	0.0	19.754908	29
TGTACGC	80	8.991528E-5	19.250275	2
CGCTTCG	400	0.0	19.249344	32
TCAACGC	3270	0.0	19.105923	4
ATCAACG	3260	0.0	19.097048	3
TCGATTT	265	0.0	19.094152	30
CAACGCA	3340	0.0	18.771362	5
TATCAAC	3460	0.0	18.375984	2
CAAGACG	875	0.0	18.353218	4
CGCGGGA	420	0.0	18.33448	44
TAGCGTT	60	0.007416331	18.332266	9
GGATCGA	265	0.0	18.263971	27
AACGCAG	3445	0.0	18.263088	6
TAACGGC	110	2.7979986E-6	18.000256	36
CGGACCA	795	0.0	17.986376	9
>>END_MODULE