FastQCFastQC Report
Thu 2 Feb 2017
SRR4062317_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062317_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1809074
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT37930.20966527626841133No Hit
TATCAACGCAGAGTACTTTTTTTTT21640.11961920850114477No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCGGT400.005276432314.2512667
CGCGTAA400.005277401314.25087210
GTTGCGT707.2678267E-613.57075718
GTATCAA19600.012.5501721
GGTATCA6800.012.1511211
TCCGATA550.00306673812.0919828
CGTTATT550.003070553312.0899752
GCACCGT1104.965368E-711.227966
CGATCAT600.00587845311.08278518
GTCTAAA1201.2802775E-711.0803321
AAGACGG1752.0008883E-1110.8572065
ATAATAC1408.358256E-910.8566063
TTGCGTG803.7749702E-410.68637919
TCGCACG909.5209E-510.55503418
GTTCCGC1106.041406E-610.364276
CGAGCCG1106.0453967E-610.36369615
GGTTAAT1303.8556936E-710.2279991
TCTATAG1409.6950316E-810.1780673
ACGGTAT750.0026453710.1345159
ACAGTCG750.002645977510.1342338
Produced by FastQC (version 0.11.5)