##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062317_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1809074
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.272962300049638	32.0	32.0	32.0	32.0	32.0
2	30.995258900409823	32.0	32.0	32.0	32.0	32.0
3	30.99965562492192	32.0	32.0	32.0	32.0	32.0
4	30.9935884325351	32.0	32.0	32.0	32.0	32.0
5	30.978738293734807	32.0	32.0	32.0	32.0	32.0
6	34.49222917359931	36.0	36.0	36.0	32.0	36.0
7	34.47081987801494	36.0	36.0	36.0	32.0	36.0
8	34.4544700769565	36.0	36.0	36.0	32.0	36.0
9	34.54657189258151	36.0	36.0	36.0	32.0	36.0
10	34.37800167378449	36.0	36.0	36.0	32.0	36.0
11	34.49298867818563	36.0	36.0	36.0	32.0	36.0
12	34.42464542633414	36.0	36.0	36.0	32.0	36.0
13	34.45780935439899	36.0	36.0	36.0	32.0	36.0
14	34.407571497904456	36.0	36.0	36.0	32.0	36.0
15	34.36008311434469	36.0	36.0	36.0	32.0	36.0
16	34.368383493433655	36.0	36.0	36.0	32.0	36.0
17	34.325107209544775	36.0	36.0	36.0	32.0	36.0
18	34.30596703064662	36.0	36.0	36.0	32.0	36.0
19	34.3084036363355	36.0	36.0	36.0	32.0	36.0
20	34.286385189328904	36.0	36.0	36.0	32.0	36.0
21	34.26536835972437	36.0	36.0	36.0	32.0	36.0
22	34.23914997396458	36.0	36.0	36.0	32.0	36.0
23	34.21725921659368	36.0	36.0	36.0	32.0	36.0
24	34.18043927445754	36.0	36.0	36.0	32.0	36.0
25	33.762464111473605	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	5.0
4	27.0
5	124.0
6	365.0
7	58.0
8	325.0
9	278.0
10	134.0
11	33.0
12	70.0
13	76.0
14	382.0
15	563.0
16	921.0
17	1283.0
18	1600.0
19	2098.0
20	2694.0
21	3290.0
22	4274.0
23	5696.0
24	7733.0
25	10628.0
26	14781.0
27	19919.0
28	27380.0
29	38652.0
30	54253.0
31	78335.0
32	118647.0
33	182961.0
34	431295.0
35	800194.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.86877090185457	16.608330339128273	11.222962328293857	26.29993643072331
2	16.697437575591408	19.085849730358394	37.36985011066472	26.846862583385477
3	19.76951604228266	22.49263845427163	27.728000627949385	30.009844875496324
4	13.111105460021427	15.037697067932118	34.79159088371126	37.05960658833519
5	15.091969244975767	36.002952747741894	33.46038258542371	15.44469542185863
6	35.597753942547655	34.08655462928547	16.254933715640984	14.060757712525884
7	30.803610459087405	29.757292244219492	19.963988370921992	19.47510892577111
8	28.09251766330813	33.35061666637154	18.86188975262515	19.69497591769518
9	26.97150661794452	14.573368508025265	17.997477560638437	40.45764731339179
10	15.727130581577164	27.205966597019472	31.69475155322476	25.372151268178612
11	37.2129337233857	21.317782900982234	21.67453136978681	19.79475200584525
12	24.355992636436795	23.1979361654787	28.90270510213457	23.54336609594993
13	29.702403683840583	19.74656992157449	24.483481937779842	26.067544456805088
14	23.09968915449691	19.064563667988004	25.384572389592293	32.451174787922795
15	25.139578223859836	27.00796427505946	21.970030326126246	25.882427174954458
16	25.623368803667606	25.913635971213466	23.398190105348444	25.06480511977049
17	24.115155550316214	25.716788896434277	25.14773004627408	25.02032550697543
18	24.488418088788197	25.420991875119668	25.564652066747755	24.525937969344376
19	25.49591356488416	24.843013498985513	25.16027866660101	24.50079426952932
20	25.39082448405082	24.76196430557315	25.198227063561575	24.648984146814453
21	25.848095060785102	24.40344953312239	24.867533125709222	24.880922280383285
22	25.27042572581551	24.525036201551202	24.99403787979812	25.210500192835163
23	24.671595114187255	24.658151881969527	25.191676702989213	25.478576300854005
24	24.768992007878698	24.809768701536182	25.29200704877988	25.129232241805237
25	24.768899659186474	24.60795379099721	25.273093436020005	25.350053113796307
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	310.0
1	310.0
2	478.5
3	647.0
4	647.0
5	647.0
6	1344.5
7	2042.0
8	2042.0
9	2042.0
10	2617.0
11	3192.0
12	3192.0
13	3192.0
14	4190.5
15	5189.0
16	5189.0
17	5189.0
18	8521.0
19	11853.0
20	11853.0
21	11853.0
22	17637.0
23	23421.0
24	23421.0
25	23421.0
26	33521.0
27	43621.0
28	43621.0
29	43621.0
30	57038.5
31	70456.0
32	70456.0
33	70456.0
34	86501.0
35	102546.0
36	102546.0
37	102546.0
38	121851.0
39	141156.0
40	141156.0
41	141156.0
42	163440.0
43	185724.0
44	185724.0
45	185724.0
46	208224.0
47	230724.0
48	230724.0
49	230724.0
50	239595.5
51	248467.0
52	248467.0
53	248467.0
54	241917.5
55	235368.0
56	235368.0
57	235368.0
58	215774.0
59	196180.0
60	196180.0
61	196180.0
62	169372.0
63	142564.0
64	142564.0
65	142564.0
66	114611.5
67	86659.0
68	86659.0
69	86659.0
70	65601.5
71	44544.0
72	44544.0
73	44544.0
74	31919.0
75	19294.0
76	19294.0
77	19294.0
78	14290.0
79	9286.0
80	9286.0
81	9286.0
82	6279.0
83	3272.0
84	3272.0
85	3272.0
86	2273.0
87	1274.0
88	1274.0
89	1274.0
90	847.0
91	420.0
92	420.0
93	420.0
94	265.5
95	111.0
96	111.0
97	111.0
98	432.5
99	754.0
100	754.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.00132664556563192
2	3.3166139140798E-4
3	6.080458842479634E-4
4	0.0099498417422394
5	0.032558093256550034
6	0.061136249816204316
7	0.10392056930783375
8	0.10712662942477755
9	0.11569454870281701
10	0.12984543473622417
11	0.13703143155006375
12	0.12934794264911217
13	0.12514689835794446
14	0.11425734934004911
15	0.13161429549040007
16	0.11707647116701693
17	0.12332276070520055
18	0.11232265789016922
19	0.09690040318969816
20	0.09269935889853041
21	0.09070939055008252
22	0.10121200127800191
23	0.08142287159065908
24	0.09225714370998644
25	0.09032245226010654
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1809074.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.20124736822456
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.94108714911816	38.805568207274604
2	15.14191757892143	16.111378054909338
3	5.06097990955547	8.077513322816257
4	2.1719260359546353	4.621966972172397
5	1.201399496573388	3.1957975902630635
6	0.7327032633602907	2.3388436536921726
7	0.5147829469655819	1.917096643172168
8	0.34968707788787157	1.4883030985747412
9	0.2585630391345	1.2380288584747015
>10	1.4858528577487364	14.382300270397952
>50	0.10140312199243787	3.710899379062608
>100	0.03865580628636506	3.407242856338962
>500	6.250299006923333E-4	0.22750163387466987
>1k	4.1668660046155547E-4	0.47755945897637764
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	3793	0.20966527626841133	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2164	0.11961920850114477	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.763844928399833E-4
2	0.0	0.0	0.0	0.0	2.763844928399833E-4
3	0.0	0.0	0.0	0.0	2.763844928399833E-4
4	0.0	0.0	0.0	0.0	2.763844928399833E-4
5	0.0	0.0	0.0	0.0	3.3166139140798E-4
6	1.1055379713599333E-4	0.0	0.0	0.0	3.3166139140798E-4
7	1.6583069570399E-4	0.0	0.0	0.0	3.3166139140798E-4
8	1.6583069570399E-4	0.0	0.0	0.0	3.3166139140798E-4
9	1.6583069570399E-4	0.0	0.0	5.5276898567996666E-5	3.3166139140798E-4
10	2.2110759427198666E-4	0.0	0.0	1.1055379713599333E-4	3.3166139140798E-4
11	2.2110759427198666E-4	0.0	0.0	1.1055379713599333E-4	3.3166139140798E-4
12	2.2110759427198666E-4	0.0	0.0	1.1055379713599333E-4	0.0012160917684959267
13	2.2110759427198666E-4	0.0	0.0	1.1055379713599333E-4	0.0013819224641999166
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACCGGT	40	0.0052764323	14.251266	7
CGCGTAA	40	0.0052774013	14.250872	10
GTTGCGT	70	7.2678267E-6	13.570757	18
GTATCAA	1960	0.0	12.550172	1
GGTATCA	680	0.0	12.151121	1
TCCGATA	55	0.003066738	12.091982	8
CGTTATT	55	0.0030705533	12.089975	2
GCACCGT	110	4.965368E-7	11.22796	6
CGATCAT	60	0.005878453	11.082785	18
GTCTAAA	120	1.2802775E-7	11.080332	1
AAGACGG	175	2.0008883E-11	10.857206	5
ATAATAC	140	8.358256E-9	10.856606	3
TTGCGTG	80	3.7749702E-4	10.686379	19
TCGCACG	90	9.5209E-5	10.555034	18
GTTCCGC	110	6.041406E-6	10.36427	6
CGAGCCG	110	6.0453967E-6	10.363696	15
GGTTAAT	130	3.8556936E-7	10.227999	1
TCTATAG	140	9.6950316E-8	10.178067	3
ACGGTAT	75	0.00264537	10.134515	9
ACAGTCG	75	0.0026459775	10.134233	8
>>END_MODULE