FastQCFastQC Report
Thu 2 Feb 2017
SRR4062317_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062317_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1809074
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTAGG551.5942485E-423.99954815
GTATCAA20050.023.4866941
CTAGCGG1900.020.84228929
AGCGTAT1101.2667624E-719.9996248
TCTAGCG2000.019.80017328
ATACGAA2000.019.80017340
AATAGCG1001.1242719E-619.7996275
TACGAAT1900.019.68438341
TAGCGTA808.9930145E-519.2496387
ATAGCGT1051.7940147E-618.8567876
GGTATCA10500.017.813621
CAATACG2150.017.39550238
CGCAATA2300.017.21706636
GTATAGG1802.8558134E-1017.1150461
GTATTAC1551.436274E-817.0361751
TAGCGGC2251.8189894E-1216.6223730
TATCAAC28300.016.4802552
TCAACGC28150.016.3336184
ATCAACG28350.016.2183883
CGTATAT953.347943E-416.2102210