##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062317_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1809074
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.18518645450656	32.0	32.0	32.0	32.0	32.0
2	31.227383733335397	32.0	32.0	32.0	32.0	32.0
3	31.446384725002957	32.0	32.0	32.0	32.0	32.0
4	31.560450263504976	32.0	32.0	32.0	32.0	32.0
5	31.505396683607195	32.0	32.0	32.0	32.0	32.0
6	35.13965818977002	36.0	36.0	36.0	36.0	36.0
7	35.15587698457885	36.0	36.0	36.0	36.0	36.0
8	35.086207639930706	36.0	36.0	36.0	36.0	36.0
9	35.211905096198386	36.0	36.0	36.0	36.0	36.0
10	35.047692908084464	36.0	36.0	36.0	36.0	36.0
11	35.21127051740282	36.0	36.0	36.0	36.0	36.0
12	35.12145219045766	36.0	36.0	36.0	36.0	36.0
13	35.163914245630636	36.0	36.0	36.0	36.0	36.0
14	35.11933619078048	36.0	36.0	36.0	36.0	36.0
15	35.08219287878771	36.0	36.0	36.0	36.0	36.0
16	35.098574740447326	36.0	36.0	36.0	36.0	36.0
17	35.0864508582844	36.0	36.0	36.0	36.0	36.0
18	35.07132046560837	36.0	36.0	36.0	36.0	36.0
19	35.067027661665584	36.0	36.0	36.0	36.0	36.0
20	35.070499603664636	36.0	36.0	36.0	36.0	36.0
21	35.057934611851145	36.0	36.0	36.0	36.0	36.0
22	35.04387051054849	36.0	36.0	36.0	36.0	36.0
23	35.00813620670022	36.0	36.0	36.0	36.0	36.0
24	34.989387941012914	36.0	36.0	36.0	36.0	36.0
25	34.97737129603322	36.0	36.0	36.0	36.0	36.0
26	34.922564803872035	36.0	36.0	36.0	32.0	36.0
27	34.90934256973457	36.0	36.0	36.0	32.0	36.0
28	34.8906506864838	36.0	36.0	36.0	32.0	36.0
29	34.86669810079632	36.0	36.0	36.0	32.0	36.0
30	34.85288163999925	36.0	36.0	36.0	32.0	36.0
31	34.84526282506962	36.0	36.0	36.0	32.0	36.0
32	34.8203158079769	36.0	36.0	36.0	32.0	36.0
33	34.794506471266516	36.0	36.0	36.0	32.0	36.0
34	34.77893718001585	36.0	36.0	36.0	32.0	36.0
35	34.769682168888615	36.0	36.0	36.0	32.0	36.0
36	34.741846933845714	36.0	36.0	36.0	32.0	36.0
37	34.7282676109435	36.0	36.0	36.0	32.0	36.0
38	34.71072493441396	36.0	36.0	36.0	32.0	36.0
39	34.68822226177591	36.0	36.0	36.0	32.0	36.0
40	34.67852116607723	36.0	36.0	36.0	32.0	36.0
41	34.64352701990079	36.0	36.0	36.0	32.0	36.0
42	34.62068107772264	36.0	36.0	36.0	32.0	36.0
43	34.60853287372435	36.0	36.0	36.0	32.0	36.0
44	34.57179142478417	36.0	36.0	36.0	32.0	36.0
45	34.54823793830435	36.0	36.0	36.0	32.0	36.0
46	34.532482363905515	36.0	36.0	36.0	32.0	36.0
47	34.50294460038672	36.0	36.0	36.0	32.0	36.0
48	34.47845638155211	36.0	36.0	36.0	32.0	36.0
49	34.45912218073998	36.0	36.0	36.0	32.0	36.0
50	33.979427596659946	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	5.0
20	17.0
21	51.0
22	109.0
23	294.0
24	819.0
25	1994.0
26	4319.0
27	8376.0
28	14708.0
29	24145.0
30	37188.0
31	56957.0
32	89899.0
33	157418.0
34	355540.0
35	1057235.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.32824382740282	17.220127858769253	11.704186429188338	26.747441884639585
2	16.178031425374687	19.60841002463261	36.87766171508427	27.335896834908436
3	19.184376953696123	22.9274030704157	28.011006754295437	29.87721322159274
4	12.848507026246578	15.499863466060537	34.911728320676765	36.73990118701612
5	14.909395635557196	36.334610966715566	33.12130957605935	15.63468382166788
6	35.79201700762435	34.28102047957211	16.07073464778331	13.856227865020223
7	30.99298315049578	29.67098084434357	19.891005011403625	19.445030993757026
8	28.159655160595975	33.46850377596494	18.662033725541356	19.70980733789773
9	27.125258557693048	14.304500534527609	17.85781012827557	40.71243077950377
10	15.672880158578367	27.170696168315946	31.859337981751988	25.2970856913537
11	37.76484544026392	20.970120625248054	21.55439744311178	19.710636491376253
12	24.506626041831346	23.110773799192295	28.816289438685207	23.566310720291153
13	29.843610598571424	19.60748979864837	24.494741508639226	26.054158094140977
14	23.185839827447634	18.838256478176127	25.26972362656254	32.7061800678137
15	25.275693531607885	26.85318566294137	21.94796896091592	25.923151844534832
16	25.863544301558385	25.655258875347144	23.3789888206888	25.102208002405675
17	24.309674452233573	25.593425144576727	25.03413348486574	25.06276691832396
18	24.679476194950997	25.29299584869574	25.447882060948444	24.579645895404823
19	25.679104337357124	24.767975218260833	25.112460850136586	24.440459594245453
20	25.694638681753606	24.704793070472675	24.959399028229974	24.641169219543745
21	26.038473848208447	24.318311090818337	24.76622004750499	24.87699501346822
22	25.474248151264128	24.491480171623714	24.899202575461256	25.1350691016509
23	24.869961096118786	24.53957107337787	25.066967962615127	25.523499867888212
24	25.041043369436757	24.640227919220013	25.215277309456503	25.103451401886723
25	25.17800572780173	24.273342505628577	25.2075236405868	25.34112812598289
26	24.886212001109964	24.741772826419822	25.248332564981048	25.12368260748917
27	25.113981342879317	24.872807434546406	24.744785712864303	25.268425509709974
28	25.016030495530256	24.70995858604396	24.96296402756803	25.311046890857753
29	24.940646331736012	24.97458640373982	24.964139052585555	25.120628211938612
30	25.121333999610844	24.906138892328375	24.9913767180231	24.981150390037676
31	25.26089386308648	24.549975705735932	24.933708996174275	25.255421435003306
32	25.081741301150156	24.695684667349525	24.89451710030784	25.32805693119248
33	24.91420996889006	24.638377330205387	25.156655250106684	25.29075745079787
34	25.148295493176832	24.6496379052733	25.17013007329233	25.031936528257543
35	25.27295762689379	24.813163876285742	25.08578984142146	24.82808865539901
36	25.135014123278808	24.77228631285688	25.109531416694765	24.98316814716954
37	25.366491915066742	24.623447522558877	24.69868062910576	25.311379933268626
38	25.12274235327024	24.87344354072857	24.949228168665293	25.0545859373359
39	25.1673105550338	24.694523844137926	24.910713347974447	25.227452252853826
40	25.37979396077439	24.551469177860703	25.222918035922266	24.845818825442645
41	25.112503240635046	24.699249720434768	25.123392879944767	25.064854158985415
42	25.315576124396284	24.679863327276557	24.930886963078354	25.073673585248812
43	25.223671940082177	24.703387652148052	24.836056828841837	25.236883578927934
44	24.957725519634764	24.988073593803463	25.03290471976124	25.021296166800532
45	25.21530804251546	24.981592406121273	24.773249399127494	25.029850152235777
46	25.110970336785986	24.823138434596874	24.889582441977577	25.176308786639556
47	25.03646919589776	24.945980262688767	25.042881362209457	24.974669179204017
48	25.259422802230603	25.051685557231114	24.70254547223463	24.98634616830365
49	25.116928273121008	24.884315497660328	24.727932051420247	25.270824177798417
50	25.025718017758557	25.146610666523685	24.760107968688416	25.067563347029342
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	157.0
1	106.5
2	56.0
3	155.5
4	255.0
5	281.5
6	308.0
7	268.5
8	229.0
9	301.5
10	374.0
11	600.5
12	827.0
13	1436.5
14	2046.0
15	2764.0
16	3482.0
17	3475.0
18	3468.0
19	3484.5
20	3501.0
21	4209.0
22	4917.0
23	6087.5
24	7258.0
25	9018.0
26	10778.0
27	13323.5
28	15869.0
29	18643.0
30	21417.0
31	24631.0
32	27845.0
33	32243.5
34	36642.0
35	42207.0
36	47772.0
37	53681.0
38	59590.0
39	65772.5
40	71955.0
41	78174.0
42	84393.0
43	90129.0
44	95865.0
45	104794.5
46	113724.0
47	120692.5
48	127661.0
49	131851.0
50	136041.0
51	139418.5
52	142796.0
53	143552.5
54	144309.0
55	141998.0
56	139687.0
57	134802.5
58	129918.0
59	119166.0
60	108414.0
61	97155.0
62	85896.0
63	74081.5
64	62267.0
65	52275.5
66	42284.0
67	35521.5
68	28759.0
69	24333.0
70	19907.0
71	15646.0
72	11385.0
73	9437.5
74	7490.0
75	5751.0
76	4012.0
77	3218.5
78	2425.0
79	1922.0
80	1419.0
81	1122.5
82	826.0
83	641.5
84	457.0
85	331.0
86	205.0
87	147.0
88	89.0
89	64.5
90	40.0
91	26.5
92	13.0
93	12.0
94	11.0
95	15.5
96	20.0
97	14.0
98	8.0
99	7.5
100	7.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.028522879661086277
2	0.0049749208711197
3	1.6583069570399E-4
4	0.0
5	0.0
6	1.6583069570399E-4
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	9.9498417422394E-4
17	0.0
18	2.2110759427198666E-4
19	0.0
20	2.2110759427198666E-4
21	5.5276898567996666E-5
22	0.0
23	0.0
24	6.6332278281596E-4
25	1.6583069570399E-4
26	4.4221518854397333E-4
27	3.3166139140798E-4
28	0.0012160917684959267
29	1.6583069570399E-4
30	9.9498417422394E-4
31	2.7638449283998336E-4
32	7.185996813839567E-4
33	3.3166139140798E-4
34	9.397072756559434E-4
35	1.1055379713599333E-4
36	2.2110759427198666E-4
37	0.0016583069570398998
38	0.0
39	7.185996813839567E-4
40	5.5276898567996666E-5
41	8.291534785199499E-4
42	0.00447742878400773
43	0.0034824446097837898
44	0.0034824446097837898
45	0.002100522145583873
46	0.0017688607541758933
47	7.185996813839567E-4
48	0.0032060601169438064
49	0.003261337015511803
50	0.0017135838556078967
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1809074.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.27405541442237
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.3801084656295	40.5601618164553
2	15.108528429060279	16.70219275236507
3	4.984020027119519	8.264609974967856
4	2.1651212849596293	4.787001355352157
5	1.1561860645911715	3.195354630179767
6	0.6591702929968821	2.186100918159042
7	0.46856285736766606	1.8129558540296322
8	0.3310776394611791	1.4640003032042692
9	0.25045376154290905	1.2459235584845954
>10	1.380376797601946	13.779831739983061
>50	0.08693055147593372	3.262090570559553
>100	0.029061700459080252	2.589964991535078
>500	3.0159580075112815E-4	0.0889089226851997
>1k	1.0053193358370933E-4	0.060902612039361766
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	5.5276898567996666E-5
2	1.1055379713599333E-4	0.0	0.0	0.0	5.5276898567996666E-5
3	1.1055379713599333E-4	0.0	0.0	0.0	5.5276898567996666E-5
4	1.1055379713599333E-4	0.0	0.0	0.0	5.5276898567996666E-5
5	1.1055379713599333E-4	0.0	0.0	0.0	5.5276898567996666E-5
6	1.1055379713599333E-4	0.0	0.0	0.0	5.5276898567996666E-5
7	1.1055379713599333E-4	0.0	0.0	0.0	5.5276898567996666E-5
8	1.1055379713599333E-4	0.0	0.0	0.0	1.1055379713599333E-4
9	1.1055379713599333E-4	0.0	0.0	0.0	1.1055379713599333E-4
10	1.1055379713599333E-4	0.0	0.0	0.0	1.1055379713599333E-4
11	1.1055379713599333E-4	0.0	0.0	0.0	1.1055379713599333E-4
12	1.6583069570399E-4	0.0	0.0	0.0	6.080458842479634E-4
13	1.6583069570399E-4	0.0	0.0	0.0	8.844303770879467E-4
14	1.6583069570399E-4	0.0	0.0	0.0	8.844303770879467E-4
15	1.6583069570399E-4	0.0	0.0	1.6583069570399E-4	0.00116081486992793
16	1.6583069570399E-4	0.0	0.0	1.6583069570399E-4	0.0012160917684959267
17	1.6583069570399E-4	0.0	0.0	1.6583069570399E-4	0.00132664556563192
18	2.2110759427198666E-4	0.0	0.0	3.3166139140798E-4	0.00149247626133591
19	3.8693828997597664E-4	0.0	0.0	3.3166139140798E-4	0.0015477531599039065
20	3.8693828997597664E-4	0.0	0.0	4.9749208711197E-4	0.0016583069570399
21	3.8693828997597664E-4	0.0	0.0	6.080458842479634E-4	0.00182413765274389
22	3.8693828997597664E-4	0.0	0.0	9.9498417422394E-4	0.00198996834844788
23	3.8693828997597664E-4	0.0	0.0	0.00149247626133591	0.00198996834844788
24	5.527689856799666E-4	0.0	0.0	0.0023769066384238566	0.00198996834844788
25	5.527689856799666E-4	0.0	0.0	0.0029296756241038234	0.0020452452470158767
26	5.527689856799666E-4	0.0	0.0	0.003261337015511803	0.00232162973985586
27	5.527689856799666E-4	0.0	0.0	0.004035213595463757	0.0023769066384238566
28	5.527689856799666E-4	0.0	0.0	0.00480909017541571	0.0023769066384238566
29	5.527689856799666E-4	0.0	0.0	0.007517658205247546	0.0023769066384238566
30	5.527689856799666E-4	0.0	0.0	0.011055379713599333	0.0023769066384238566
31	5.527689856799666E-4	0.0	0.0	0.018351930324574894	0.00265329113126384
32	5.527689856799666E-4	0.0	0.0	0.026035419225526428	0.0029296756241038234
33	5.527689856799666E-4	0.0	0.0	0.03333196983650199	0.0030402294212398167
34	5.527689856799666E-4	0.0	0.0	0.041678781520269485	0.0033718908126477965
35	6.080458842479634E-4	0.0	0.0	0.05118640807396491	0.0033718908126477965
36	6.080458842479634E-4	0.0	0.0	0.06356843335319616	0.0033718908126477965
37	6.080458842479634E-4	0.0	0.0	0.082915347851995	0.0033718908126477965
38	7.185996813839566E-4	0.0	0.0	0.1077346753090255	0.0035929984069197835
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTAGG	55	1.5942485E-4	23.999548	15
GTATCAA	2005	0.0	23.486694	1
CTAGCGG	190	0.0	20.842289	29
AGCGTAT	110	1.2667624E-7	19.999624	8
TCTAGCG	200	0.0	19.800173	28
ATACGAA	200	0.0	19.800173	40
AATAGCG	100	1.1242719E-6	19.799627	5
TACGAAT	190	0.0	19.684383	41
TAGCGTA	80	8.9930145E-5	19.249638	7
ATAGCGT	105	1.7940147E-6	18.856787	6
GGTATCA	1050	0.0	17.81362	1
CAATACG	215	0.0	17.395502	38
CGCAATA	230	0.0	17.217066	36
GTATAGG	180	2.8558134E-10	17.115046	1
GTATTAC	155	1.436274E-8	17.036175	1
TAGCGGC	225	1.8189894E-12	16.62237	30
TATCAAC	2830	0.0	16.480255	2
TCAACGC	2815	0.0	16.333618	4
ATCAACG	2835	0.0	16.218388	3
CGTATAT	95	3.347943E-4	16.21022	10
>>END_MODULE