FastQCFastQC Report
Thu 2 Feb 2017
SRR4062316_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062316_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1431136
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT17240.1204637434876909No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAT400.00527495914.25157512
CGTCTTA551.9593052E-413.81874115
TACCGTA852.6948692E-713.4132477
GGTATCA4350.013.3153911
GTCCTAA1457.2759576E-1212.442251
GTATCAA10900.012.3701551
CGACTTT550.00306650412.09182213
ACGACGT658.015293E-411.6936016
AAGACGG2450.011.6331245
ACGATCA1157.059862E-811.5664969
CGAACGT752.0741599E-411.3996644
TCGAACG752.0768576E-411.3980693
CGAGCCG1604.5474735E-1111.28170815
AACCGCG600.00587043611.0845587
CGTATAT1201.2727105E-711.08455810
ACACCGT600.00587043611.0845586
ACCGTAT600.00587043611.0845588
CTAGATC600.005882679511.0814563
CTAGGAC1658.0035534E-1110.9375413
CGACGTT700.001489901210.8583437
Produced by FastQC (version 0.11.5)