##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062316_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1431136
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.209306453055476	32.0	32.0	32.0	32.0	32.0
2	30.79525775328131	32.0	32.0	32.0	32.0	32.0
3	30.804164663595913	32.0	32.0	32.0	32.0	32.0
4	30.823279548554435	32.0	32.0	32.0	32.0	32.0
5	30.685983722022225	32.0	32.0	32.0	32.0	32.0
6	34.346405932070745	36.0	36.0	36.0	32.0	36.0
7	34.287384287726674	36.0	36.0	36.0	32.0	36.0
8	34.22773447107752	36.0	36.0	36.0	32.0	36.0
9	34.38516255617915	36.0	36.0	36.0	32.0	36.0
10	34.03749608702457	36.0	36.0	36.0	32.0	36.0
11	34.36281108154641	36.0	36.0	36.0	32.0	36.0
12	34.176254388122445	36.0	36.0	36.0	32.0	36.0
13	34.267110183798046	36.0	36.0	36.0	32.0	36.0
14	34.171173808778484	36.0	36.0	36.0	32.0	36.0
15	34.104297565011294	36.0	36.0	36.0	32.0	36.0
16	34.10470143997496	36.0	36.0	36.0	32.0	36.0
17	34.03669602329897	36.0	36.0	36.0	32.0	36.0
18	34.03136669051718	36.0	36.0	36.0	32.0	36.0
19	34.048084179281354	36.0	36.0	36.0	32.0	36.0
20	34.02754035954654	36.0	36.0	36.0	32.0	36.0
21	34.015913931310514	36.0	36.0	36.0	32.0	36.0
22	33.977749843480986	36.0	36.0	36.0	32.0	36.0
23	33.946109244683946	36.0	36.0	36.0	32.0	36.0
24	33.923221832166895	36.0	36.0	36.0	32.0	36.0
25	33.56491416608904	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1.0
3	1.0
4	19.0
5	118.0
6	310.0
7	61.0
8	232.0
9	183.0
10	96.0
11	25.0
12	54.0
13	68.0
14	130.0
15	236.0
16	366.0
17	501.0
18	675.0
19	946.0
20	1424.0
21	2107.0
22	3189.0
23	4835.0
24	7229.0
25	10785.0
26	15842.0
27	21262.0
28	29338.0
29	40609.0
30	55230.0
31	77519.0
32	110214.0
33	152890.0
34	312924.0
35	581717.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.53867535730806	17.416243803795354	11.562987817915783	26.482093020980802
2	16.84702518497608	19.199011131755235	37.300289072069575	26.65367461119911
3	19.607427997255307	22.165902932376326	28.126943399812454	30.09972567055591
4	13.20186166123915	15.19944373785797	34.99769388810465	36.60100071279822
5	15.220030741464841	35.71643759685344	33.616330022696125	15.447201638985602
6	35.28626205052621	34.50771485385547	16.264259964145577	13.941763131472745
7	30.800481808376247	29.861976729382896	19.96657839109018	19.370963071150673
8	27.942761651677632	33.492142213107016	18.990123817764538	19.574972317450815
9	26.5856292988141	14.955465824952569	18.75905933972454	39.69984553650879
10	16.49112127954326	26.694373312063586	31.235394539831802	25.579110868561354
11	37.0737615618696	21.428196582745517	21.689539092571223	19.808502762813653
12	24.037890379848328	23.662176996685748	28.676586339565134	23.623346283900787
13	29.283343069727742	19.599257002333236	24.87950774655524	26.23789218138378
14	23.136566341966738	19.493608016648885	25.027753974222204	32.34207166716217
15	25.110685409955806	27.057055460247003	21.983017975534466	25.84924115426272
16	25.798287053845943	25.919311183818866	23.498688672071477	24.783713090263713
17	24.104234527687296	26.16203252851562	25.07723563585077	24.656497307946314
18	24.62419440249201	25.585280186690863	25.62060650745805	24.16991890335908
19	25.513338620941443	25.212168772617467	25.04255964995475	24.231932956486343
20	25.368119655848158	24.82141283533613	25.02094648749435	24.789521021321356
21	25.921395510832667	24.54348102797372	24.827638298497316	24.707485162696297
22	25.454305100821074	24.58888544607966	25.081712318083866	24.875097135015398
23	24.556645836946366	24.674477345581284	25.30482346951905	25.464053347953296
24	24.472130312878754	24.99442249577054	25.348655209528825	25.18479198182188
25	24.78931710066761	24.73266875263135	25.301320256190408	25.17669389051063
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	92.0
1	92.0
2	223.0
3	354.0
4	354.0
5	354.0
6	741.5
7	1129.0
8	1129.0
9	1129.0
10	1613.0
11	2097.0
12	2097.0
13	2097.0
14	3111.5
15	4126.0
16	4126.0
17	4126.0
18	6988.0
19	9850.0
20	9850.0
21	9850.0
22	14871.5
23	19893.0
24	19893.0
25	19893.0
26	28492.0
27	37091.0
28	37091.0
29	37091.0
30	47251.5
31	57412.0
32	57412.0
33	57412.0
34	71656.0
35	85900.0
36	85900.0
37	85900.0
38	101797.5
39	117695.0
40	117695.0
41	117695.0
42	134690.5
43	151686.0
44	151686.0
45	151686.0
46	166417.5
47	181149.0
48	181149.0
49	181149.0
50	188160.5
51	195172.0
52	195172.0
53	195172.0
54	185693.5
55	176215.0
56	176215.0
57	176215.0
58	161152.0
59	146089.0
60	146089.0
61	146089.0
62	127299.5
63	108510.0
64	108510.0
65	108510.0
66	88544.0
67	68578.0
68	68578.0
69	68578.0
70	51655.5
71	34733.0
72	34733.0
73	34733.0
74	26079.0
75	17425.0
76	17425.0
77	17425.0
78	13470.0
79	9515.0
80	9515.0
81	9515.0
82	6579.5
83	3644.0
84	3644.0
85	3644.0
86	2624.0
87	1604.0
88	1604.0
89	1604.0
90	1047.0
91	490.0
92	490.0
93	490.0
94	315.0
95	140.0
96	140.0
97	140.0
98	343.5
99	547.0
100	547.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0012577421013796033
2	3.493728059387787E-4
3	6.987456118775574E-4
4	0.010900431545289895
5	0.03458790778793909
6	0.06344610155848222
7	0.10634908212776423
8	0.10725745142320506
9	0.1168302663059276
10	0.13108467678822977
11	0.1381420074681931
12	0.12961731100328688
13	0.12514533908727055
14	0.1125679180734745
15	0.12996668380922566
16	0.11662064262236434
17	0.12186123471144603
18	0.11228841982872347
19	0.09628714531672741
20	0.09097667866645798
21	0.09041768217695594
22	0.09957124969255193
23	0.07881850501978847
24	0.08978881112626613
25	0.08818169621894774
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1431136.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.69229350231215
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.99865245822603	34.97699941676425
2	16.24019839729033	16.465058073824416
3	6.33553285240622	9.63488072543151
4	3.0268343997552285	6.137487111011474
5	1.656077432576697	4.197518163736674
6	0.9491202895738751	2.886785057284702
7	0.6264282391980751	2.22285589116958
8	0.4370981953211305	1.7726008005239762
9	0.2934369634079306	1.3387493406151842
>10	1.3084948207999327	11.372201290658767
>50	0.07389850719545382	2.5895159668160526
>100	0.05034419777869268	4.920180162142077
>500	0.0034671843486768247	1.1982581487509738
>1k	4.16062121841219E-4	0.2869098512704016
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	1724	0.1204637434876909	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.891219283142902E-4
2	0.0	0.0	0.0	0.0	4.891219283142902E-4
3	0.0	0.0	0.0	0.0	4.891219283142902E-4
4	0.0	0.0	0.0	0.0	4.891219283142902E-4
5	0.0	0.0	0.0	0.0	4.891219283142902E-4
6	0.0	0.0	0.0	0.0	5.58996489502046E-4
7	0.0	0.0	0.0	0.0	5.58996489502046E-4
8	0.0	0.0	0.0	0.0	5.58996489502046E-4
9	0.0	0.0	0.0	0.0	5.58996489502046E-4
10	0.0	0.0	0.0	0.0	5.58996489502046E-4
11	0.0	0.0	0.0	0.0	5.58996489502046E-4
12	0.0	0.0	0.0	0.0	0.001327616662567359
13	0.0	0.0	0.0	0.0	0.0014673657849428705
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGAAAT	40	0.005274959	14.251575	12
CGTCTTA	55	1.9593052E-4	13.818741	15
TACCGTA	85	2.6948692E-7	13.413247	7
GGTATCA	435	0.0	13.315391	1
GTCCTAA	145	7.2759576E-12	12.44225	1
GTATCAA	1090	0.0	12.370155	1
CGACTTT	55	0.003066504	12.091822	13
ACGACGT	65	8.015293E-4	11.693601	6
AAGACGG	245	0.0	11.633124	5
ACGATCA	115	7.059862E-8	11.566496	9
CGAACGT	75	2.0741599E-4	11.399664	4
TCGAACG	75	2.0768576E-4	11.398069	3
CGAGCCG	160	4.5474735E-11	11.281708	15
AACCGCG	60	0.005870436	11.084558	7
CGTATAT	120	1.2727105E-7	11.084558	10
ACACCGT	60	0.005870436	11.084558	6
ACCGTAT	60	0.005870436	11.084558	8
CTAGATC	60	0.0058826795	11.081456	3
CTAGGAC	165	8.0035534E-11	10.937541	3
CGACGTT	70	0.0014899012	10.858343	7
>>END_MODULE