FastQCFastQC Report
Thu 2 Feb 2017
SRR4062316_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062316_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1431136
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA11150.026.6470551
GGTATCA5250.023.0565171
ACGACGG2950.018.6434466
GACGGTA3200.018.561888
GTATAGG2850.018.5334681
CGACGGT3000.018.3327227
TACGACG3150.018.1581255
TCAACGC16750.017.4680734
GTATTAG2300.017.224041
ATCAACG17050.017.1607193
CAACGCA17400.016.815535
CTACGAC3550.016.7318364
ACGGACC4350.016.6890988
AACGCAG17650.016.452716
ACGGTAT3350.016.4173629
CGCGGGA2450.016.16385544
TATCAAC18600.015.967212
CTAGACA2800.015.7137624
TAGACCC1401.5871774E-615.7137624
TATAGGG2101.5097612E-1015.7137612
Produced by FastQC (version 0.11.5)