##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062316_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1431136
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.954677962122396	32.0	32.0	32.0	32.0	32.0
2	31.183638731748765	32.0	32.0	32.0	32.0	32.0
3	31.419153735214543	32.0	32.0	32.0	32.0	32.0
4	31.552062138049774	32.0	32.0	32.0	32.0	32.0
5	31.47074911119558	32.0	32.0	32.0	32.0	32.0
6	35.13420527469088	36.0	36.0	36.0	36.0	36.0
7	35.140981709634865	36.0	36.0	36.0	36.0	36.0
8	35.060871223978715	36.0	36.0	36.0	36.0	36.0
9	35.21484820450328	36.0	36.0	36.0	36.0	36.0
10	35.026841613934664	36.0	36.0	36.0	36.0	36.0
11	35.2218678029202	36.0	36.0	36.0	36.0	36.0
12	35.112600759117235	36.0	36.0	36.0	36.0	36.0
13	35.17232184781879	36.0	36.0	36.0	36.0	36.0
14	35.12625774210138	36.0	36.0	36.0	36.0	36.0
15	35.09072582899179	36.0	36.0	36.0	36.0	36.0
16	35.10723788654607	36.0	36.0	36.0	36.0	36.0
17	35.08784280459719	36.0	36.0	36.0	36.0	36.0
18	35.08564804463028	36.0	36.0	36.0	36.0	36.0
19	35.08548523690271	36.0	36.0	36.0	36.0	36.0
20	35.08389698812692	36.0	36.0	36.0	36.0	36.0
21	35.07113509827158	36.0	36.0	36.0	36.0	36.0
22	35.050721943966195	36.0	36.0	36.0	36.0	36.0
23	35.02067937638352	36.0	36.0	36.0	36.0	36.0
24	35.002019374818325	36.0	36.0	36.0	36.0	36.0
25	34.985654053842545	36.0	36.0	36.0	36.0	36.0
26	34.94415205822507	36.0	36.0	36.0	32.0	36.0
27	34.93263393555888	36.0	36.0	36.0	32.0	36.0
28	34.91074293428437	36.0	36.0	36.0	32.0	36.0
29	34.88509268161796	36.0	36.0	36.0	32.0	36.0
30	34.86711745075241	36.0	36.0	36.0	32.0	36.0
31	34.86964830735863	36.0	36.0	36.0	32.0	36.0
32	34.845080411645014	36.0	36.0	36.0	32.0	36.0
33	34.81758337432641	36.0	36.0	36.0	32.0	36.0
34	34.79948516423317	36.0	36.0	36.0	32.0	36.0
35	34.80060525344901	36.0	36.0	36.0	32.0	36.0
36	34.773208835498515	36.0	36.0	36.0	32.0	36.0
37	34.765206800751294	36.0	36.0	36.0	32.0	36.0
38	34.749682070746594	36.0	36.0	36.0	32.0	36.0
39	34.734550734521385	36.0	36.0	36.0	32.0	36.0
40	34.71836568991347	36.0	36.0	36.0	32.0	36.0
41	34.68622618674955	36.0	36.0	36.0	32.0	36.0
42	34.66929767681059	36.0	36.0	36.0	32.0	36.0
43	34.658739630615116	36.0	36.0	36.0	32.0	36.0
44	34.6157521018268	36.0	36.0	36.0	32.0	36.0
45	34.60680186928426	36.0	36.0	36.0	32.0	36.0
46	34.58511629921964	36.0	36.0	36.0	32.0	36.0
47	34.56961602531136	36.0	36.0	36.0	32.0	36.0
48	34.553176637300716	36.0	36.0	36.0	32.0	36.0
49	34.530280141090714	36.0	36.0	36.0	32.0	36.0
50	34.06883133398922	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	0.0
19	1.0
20	7.0
21	32.0
22	69.0
23	207.0
24	523.0
25	1397.0
26	3167.0
27	6069.0
28	10973.0
29	17944.0
30	28581.0
31	44645.0
32	71364.0
33	126626.0
34	283091.0
35	836439.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.78351452187612	18.355352272743158	12.069575282127126	26.791557923253595
2	16.26114372841656	19.72665284953818	36.871678194139506	27.140525227905755
3	18.97330225304165	22.640678847234216	28.437628394865044	29.94839050485909
4	13.066482197696233	15.780202426745205	35.13344303647105	36.019872339087506
5	15.005072893142232	36.08951210786396	33.29697526999531	15.608439728998501
6	35.42498466251239	34.809039544864426	16.046296153959027	13.719679638664164
7	30.862894931019834	29.960814346085908	19.915577555172952	19.260713167721306
8	27.99803792232185	33.73369127741878	18.770612995550387	19.497657804708986
9	26.59719271962972	14.724596404534578	18.51557084721508	40.16264002862062
10	16.336124824003324	26.843728928437915	31.410314191183918	25.40983205637484
11	37.3367730250654	21.317191378038146	21.674529883952328	19.671505712944125
12	24.016375802159963	23.709067482056213	28.586241978400373	23.688314737383447
13	29.44192585470563	19.457130559220086	24.883309482816447	26.21763410325783
14	23.22588489144288	19.34812624376719	24.90462122397871	32.521367640811214
15	25.17126254947119	27.09351172774635	21.876117992979005	25.859107729803455
16	25.85367796518977	25.93074987544816	23.48415344227747	24.7314187170846
17	24.204408246316213	26.167184670080275	24.945637591395926	24.68276949220759
18	24.6776677483279	25.583384341905568	25.56312066252449	24.175827247242044
19	25.68463095051763	25.188172193278625	24.976102900073787	24.151093956129955
20	25.560761214001477	24.760906553190303	24.846502950455406	24.831829282352818
21	26.090672025579682	24.555877289090624	24.687241464123606	24.66620922120609
22	25.541667598327482	24.579914138139213	25.0136954139928	24.864722849540506
23	24.60800371173669	24.704710104420542	25.184259217852112	25.503026965990657
24	24.67951719000896	25.058171141244422	25.21336405980762	25.048947608939
25	24.95124471832339	24.701651216139446	25.196366220468203	25.150737845068953
26	24.723155441022744	25.313459033713265	25.25818794685032	24.70519757841367
27	25.104235886492415	25.026534994399523	24.93010762831303	24.93912149079503
28	24.96029314247023	24.9403785724817	25.20793256716975	24.89139571787832
29	24.839864414927774	25.039985857339403	25.234726939742064	24.88542278799076
30	24.784626876413665	25.34160284140894	25.218482574149913	24.655287708027483
31	25.089736718721067	24.928675292478466	24.9504762317356	25.031111757064867
32	24.98055730825104	25.042326722468765	24.817888534157298	25.1592274351229
33	24.65581513973175	24.82016022248076	25.15325248840955	25.37077214937794
34	25.0738928647792	24.870696221017578	25.27890625982618	24.776504654377042
35	25.353670585703362	24.768889565896696	25.317475512425812	24.55996433597413
36	24.726300578842483	25.150370475958887	25.10362426386944	25.019704681329184
37	25.4862282495224	24.916603662311562	24.88753547925468	24.709632608911356
38	24.872129553026408	24.813155423383943	25.139818996936697	25.174896026652956
39	25.296043322560923	24.887832998515155	24.78413835269456	25.031985326229368
40	25.23610614225343	25.094959528654158	25.08566621201619	24.583268117076223
41	24.739278539610446	25.22337322036859	25.294226570006114	24.743121670014848
42	25.502772762084714	25.140594690384216	24.93040143557521	24.42623111195586
43	25.13804240427872	24.66951579294548	25.118896052078078	25.073545750697722
44	24.72630714672536	25.060741051307584	25.032300892685566	25.180650909281482
45	24.9227522692176	25.198482296958307	25.01345109739992	24.865314336424174
46	24.828332193190306	25.00637618291295	25.010848245449154	25.154443378447596
47	24.845174467395275	24.962354843420606	25.243042720876623	24.949427968307493
48	25.23943221060252	25.369193078861347	24.684542035673406	24.706832674862728
49	24.769565208276994	25.231657628158498	24.946841553239853	25.051935610324655
50	24.87742092365614	25.37856792980588	24.90760713484642	24.836404011691567
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	23.0
1	24.5
2	26.0
3	56.0
4	86.0
5	103.0
6	120.0
7	100.0
8	80.0
9	116.0
10	152.0
11	246.5
12	341.0
13	646.5
14	952.0
15	1215.5
16	1479.0
17	1629.0
18	1779.0
19	2038.0
20	2297.0
21	3019.0
22	3741.0
23	4839.5
24	5938.0
25	7733.0
26	9528.0
27	11778.0
28	14028.0
29	16165.5
30	18303.0
31	21233.0
32	24163.0
33	27743.0
34	31323.0
35	35809.5
36	40296.0
37	45833.0
38	51370.0
39	56543.5
40	61717.0
41	67508.0
42	73299.0
43	77306.5
44	81314.0
45	87859.5
46	94405.0
47	98998.5
48	103592.0
49	106379.5
50	109167.0
51	109213.0
52	109259.0
53	109262.5
54	109266.0
55	106098.5
56	102931.0
57	98587.5
58	94244.0
59	88107.5
60	81971.0
61	72202.0
62	62433.0
63	53964.5
64	45496.0
65	38460.5
66	31425.0
67	26964.5
68	22504.0
69	19573.5
70	16643.0
71	12953.5
72	9264.0
73	7976.5
74	6689.0
75	5106.0
76	3523.0
77	2953.0
78	2383.0
79	2013.5
80	1644.0
81	1283.5
82	923.0
83	744.0
84	565.0
85	427.0
86	289.0
87	188.5
88	88.0
89	55.0
90	22.0
91	17.0
92	12.0
93	12.0
94	12.0
95	11.5
96	11.0
97	11.5
98	12.0
99	10.0
100	8.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.027810075352726785
2	0.004332222793640856
3	2.79498244751023E-4
4	6.987456118775575E-5
5	0.0
6	1.397491223755115E-4
7	0.0
8	0.0
9	0.0
10	6.987456118775575E-5
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	3.493728059387787E-4
17	0.0
18	2.79498244751023E-4
19	0.0
20	6.987456118775575E-5
21	0.0
22	0.0
23	0.0
24	9.782438566285803E-4
25	6.288710506898017E-4
26	5.58996489502046E-4
27	4.891219283142902E-4
28	0.0016071149073183819
29	3.493728059387787E-4
30	0.0010481184178163362
31	3.493728059387787E-4
32	4.891219283142902E-4
33	6.987456118775575E-5
34	6.987456118775574E-4
35	1.397491223755115E-4
36	2.79498244751023E-4
37	0.0012577421013796033
38	0.0
39	7.686201730653132E-4
40	0.0
41	7.686201730653132E-4
42	0.005030968405518413
43	0.00377322630413881
44	0.0042623482324531
45	0.0018167385908816494
46	0.0020263622744449163
47	6.288710506898017E-4
48	0.0029347315698857413
49	0.0025853587639469624
50	0.001327616662567359
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1431136.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.84549130939513
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.8019351188639	35.67070129274626
2	16.4371232401285	17.04381460195078
3	6.482169687431464	10.082138165872575
4	3.0934080005758293	6.4151703044107
5	1.6183818448570597	4.195290093640977
6	0.9326373092700081	2.9011823707545514
7	0.6062524335213371	2.200201869140109
8	0.3884289616976498	1.6110632286410302
9	0.2960245091650232	1.3812782505554855
>10	1.2335629665402554	10.836769567610862
>50	0.06492853718482057	2.287981362637688
>100	0.04243587214138659	4.340372590308332
>500	0.002575942691595089	0.9517969928445583
>1k	1.355759311365836E-4	0.08223930888620733
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.79498244751023E-4
2	0.0	0.0	0.0	0.0	2.79498244751023E-4
3	0.0	0.0	0.0	0.0	2.79498244751023E-4
4	0.0	0.0	0.0	0.0	2.79498244751023E-4
5	0.0	0.0	0.0	0.0	3.493728059387787E-4
6	0.0	0.0	0.0	0.0	4.891219283142902E-4
7	0.0	0.0	0.0	0.0	4.891219283142902E-4
8	0.0	0.0	0.0	0.0	4.891219283142902E-4
9	0.0	0.0	0.0	0.0	4.891219283142902E-4
10	0.0	0.0	0.0	0.0	4.891219283142902E-4
11	0.0	0.0	0.0	0.0	4.891219283142902E-4
12	0.0	0.0	0.0	0.0	9.083692954408246E-4
13	0.0	0.0	0.0	0.0	0.0011878675401918476
14	0.0	0.0	0.0	0.0	0.0011878675401918476
15	0.0	0.0	0.0	1.397491223755115E-4	0.0012577421013796033
16	0.0	0.0	0.0	3.493728059387787E-4	0.001327616662567359
17	0.0	0.0	0.0	4.1924736712653445E-4	0.001327616662567359
18	0.0	0.0	0.0	5.58996489502046E-4	0.001607114907318382
19	0.0	0.0	0.0	7.686201730653132E-4	0.001607114907318382
20	0.0	0.0	0.0	8.384947342530689E-4	0.001607114907318382
21	0.0	0.0	0.0	9.083692954408246E-4	0.0016769894685061378
22	0.0	0.0	0.0	0.0011878675401918476	0.0016769894685061378
23	0.0	0.0	0.0	0.001327616662567359	0.0016769894685061378
24	0.0	0.0	0.0	0.0018167385908816492	0.0016769894685061378
25	0.0	0.0	0.0	0.002096236835632672	0.0016769894685061378
26	0.0	0.0	0.0	0.0025154842027592067	0.0016769894685061378
27	0.0	0.0	0.0	0.0031443552534490085	0.0016769894685061378
28	0.0	0.0	0.0	0.004471971916016368	0.0016769894685061378
29	0.0	0.0	0.0	0.007336828924714353	0.0016769894685061378
30	0.0	0.0	0.0	0.012856919258547057	0.0016769894685061378
31	0.0	0.0	0.0	0.022429734141269593	0.0017468640296938935
32	0.0	0.0	0.0	0.03353978937012275	0.0018167385908816492
33	0.0	0.0	0.0	0.04416072267066163	0.0018167385908816492
34	0.0	0.0	0.0	0.05701764192920868	0.0020263622744449163
35	0.0	0.0	0.0	0.06896619189231491	0.0020263622744449163
36	0.0	0.0	0.0	0.08685407955638039	0.0020263622744449163
37	0.0	0.0	0.0	0.11410515841960513	0.0020263622744449163
38	0.0	0.0	0.0	0.15064955392080137	0.002096236835632672
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	1115	0.0	26.647055	1
GGTATCA	525	0.0	23.056517	1
ACGACGG	295	0.0	18.643446	6
GACGGTA	320	0.0	18.56188	8
GTATAGG	285	0.0	18.533468	1
CGACGGT	300	0.0	18.332722	7
TACGACG	315	0.0	18.158125	5
TCAACGC	1675	0.0	17.468073	4
GTATTAG	230	0.0	17.22404	1
ATCAACG	1705	0.0	17.160719	3
CAACGCA	1740	0.0	16.81553	5
CTACGAC	355	0.0	16.731836	4
ACGGACC	435	0.0	16.689098	8
AACGCAG	1765	0.0	16.45271	6
ACGGTAT	335	0.0	16.417362	9
CGCGGGA	245	0.0	16.163855	44
TATCAAC	1860	0.0	15.96721	2
CTAGACA	280	0.0	15.713762	4
TAGACCC	140	1.5871774E-6	15.713762	4
TATAGGG	210	1.5097612E-10	15.713761	2
>>END_MODULE