FastQCFastQC Report
Thu 2 Feb 2017
SRR4062315_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062315_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1501847
Sequences flagged as poor quality0
Sequence length25
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT30700.20441496370802087No Hit
TATCAACGCAGAGTACTTTTTTTTT18770.12497944198044142No Hit
GCGCAAGACGGACCAGAGCGAAAGC18530.1233814096908673No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG250.00603038919.0008685
TGGACGT508.710218E-515.2006955
AACCGCG751.4767937E-512.6680897
TATTAGG905.421243E-712.6634432
GGTATCA8400.012.6596431
ACGGTAT2300.012.39228259
CGAACGA853.9425104E-612.29426916
GTATTAG1251.4224497E-912.1532581
CGCCGGT2600.012.0590487
AGAACCG951.0391068E-612.0005475
CGTCGTA2400.011.87633410
CGACCAT3400.011.73661210
CCGACCA3250.011.6932329
GTATCAA20100.011.62041951
CTAGCAC1157.080234E-811.5642023
CGGTCCA2900.011.46680510
GTCCTAT2100.011.3032531
CGTTATT1354.7439244E-911.2563942
ATAATAC855.3270982E-511.1754893
CGCAAGA2900.011.1350972
Produced by FastQC (version 0.11.5)