##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR4062315_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1501847 Sequences flagged as poor quality 0 Sequence length 25 %GC 51 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.24189281597926 32.0 32.0 32.0 32.0 32.0 2 30.820272637625536 32.0 32.0 32.0 32.0 32.0 3 30.845291164812394 32.0 32.0 32.0 32.0 32.0 4 30.851140628839023 32.0 32.0 32.0 32.0 32.0 5 30.796150340214417 32.0 32.0 32.0 32.0 32.0 6 34.4403770823526 36.0 36.0 36.0 32.0 36.0 7 34.37086600699006 36.0 36.0 36.0 32.0 36.0 8 34.34471221103082 36.0 36.0 36.0 32.0 36.0 9 34.44771138471496 36.0 36.0 36.0 32.0 36.0 10 34.173907195606475 36.0 36.0 36.0 32.0 36.0 11 34.426741871841806 36.0 36.0 36.0 32.0 36.0 12 34.27150568599864 36.0 36.0 36.0 32.0 36.0 13 34.34995242524705 36.0 36.0 36.0 32.0 36.0 14 34.25871410336739 36.0 36.0 36.0 32.0 36.0 15 34.22921043222112 36.0 36.0 36.0 32.0 36.0 16 34.22489374749891 36.0 36.0 36.0 32.0 36.0 17 34.15871989623444 36.0 36.0 36.0 32.0 36.0 18 34.16004892642193 36.0 36.0 36.0 32.0 36.0 19 34.16610280541227 36.0 36.0 36.0 32.0 36.0 20 34.15985982593433 36.0 36.0 36.0 32.0 36.0 21 34.12982880413251 36.0 36.0 36.0 32.0 36.0 22 34.10756288756445 36.0 36.0 36.0 32.0 36.0 23 34.081879845283844 36.0 36.0 36.0 32.0 36.0 24 34.06165341742534 36.0 36.0 36.0 32.0 36.0 25 33.71143398761658 36.0 36.0 36.0 27.0 36.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 1.0 4 23.0 5 116.0 6 328.0 7 69.0 8 255.0 9 214.0 10 101.0 11 36.0 12 53.0 13 69.0 14 310.0 15 445.0 16 642.0 17 848.0 18 1131.0 19 1529.0 20 2177.0 21 3118.0 22 4655.0 23 6880.0 24 9460.0 25 13069.0 26 17590.0 27 22125.0 28 28675.0 29 37097.0 30 47978.0 31 64895.0 32 92266.0 33 132485.0 34 299699.0 35 713508.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.88578326826823 16.9117123187793 11.316667876302828 25.88583653664965 2 17.07074769416797 19.236818204820207 36.92280955944825 26.76962454156357 3 19.85170102361717 22.29871403639957 27.7563853722556 30.093199567727662 4 13.147720235201673 15.19781046687399 34.527032876292715 37.12743642163163 5 15.277469718617098 36.0224733320678 32.834910263199085 15.865146686116024 6 35.40071891875685 34.12312233278809 16.30239894993887 14.173759798516189 7 31.312066807181456 29.77333658623728 19.41363739986322 19.500959206718047 8 28.5797705443223 32.711265939143786 18.682552982717166 20.026410533816748 9 26.970617980411987 14.666009777339854 18.44747896498598 39.915893277262185 10 16.826435502486714 26.30334897794397 30.380638110751573 26.489577408817745 11 37.45673211888757 21.349177718549896 21.34537687983148 19.848713282731055 12 24.672173612134394 23.51782200729029 27.932268437921515 23.877735942653803 13 29.316054514021538 19.403535370708546 24.841669716928987 26.438740398340926 14 23.718007262342283 19.403799963601625 24.593634817644812 32.284557956411284 15 25.457389439771276 26.669138080937927 21.91808861887058 25.955383860420213 16 26.023519686404185 25.90698790682791 23.00489326808976 25.06459913867815 17 24.23774735626636 25.94025715318121 24.66995830561041 25.152037184942017 18 24.88429343837305 25.162669126896255 25.39224907691395 24.56078835781675 19 25.634121241528607 24.999333486187165 24.995134449166326 24.3714108231179 20 25.904603728516246 24.271011892543235 24.679093276887517 25.145291102053 21 26.63932350330171 24.217427467749374 24.396841984596477 24.74640704435244 22 26.20165806617601 24.141006277402816 24.743218365369106 24.91411729105207 23 24.61933280865047 24.296526230700078 25.204769593806166 25.879371366843284 24 24.67957454777873 24.772947967478594 25.230417741083038 25.317059743659637 25 24.997550860464358 24.439746835611757 25.00114959610857 25.56155270781532 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 476.0 1 476.0 2 540.0 3 604.0 4 604.0 5 604.0 6 1214.5 7 1825.0 8 1825.0 9 1825.0 10 2067.0 11 2309.0 12 2309.0 13 2309.0 14 3021.5 15 3734.0 16 3734.0 17 3734.0 18 5967.0 19 8200.0 20 8200.0 21 8200.0 22 13142.0 23 18084.0 24 18084.0 25 18084.0 26 26466.5 27 34849.0 28 34849.0 29 34849.0 30 44429.5 31 54010.0 32 54010.0 33 54010.0 34 68288.5 35 82567.0 36 82567.0 37 82567.0 38 99260.0 39 115953.0 40 115953.0 41 115953.0 42 135738.5 43 155524.0 44 155524.0 45 155524.0 46 174906.0 47 194288.0 48 194288.0 49 194288.0 50 202846.0 51 211404.0 52 211404.0 53 211404.0 54 199331.5 55 187259.0 56 187259.0 57 187259.0 58 172590.5 59 157922.0 60 157922.0 61 157922.0 62 139335.0 63 120748.0 64 120748.0 65 120748.0 66 97631.0 67 74514.0 68 74514.0 69 74514.0 70 56664.0 71 38814.0 72 38814.0 73 38814.0 74 29246.5 75 19679.0 76 19679.0 77 19679.0 78 15503.5 79 11328.0 80 11328.0 81 11328.0 82 7822.5 83 4317.0 84 4317.0 85 4317.0 86 3198.0 87 2079.0 88 2079.0 89 2079.0 90 1371.5 91 664.0 92 664.0 93 664.0 94 397.0 95 130.0 96 130.0 97 130.0 98 348.0 99 566.0 100 566.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0011985242171805783 2 1.9975403619676307E-4 3 0.0010653548597160697 4 0.010453794560963932 5 0.03409135551091423 6 0.06538615451507378 7 0.10979813522948743 8 0.11239493770004534 9 0.12304848629720604 10 0.13822979304816005 11 0.14502143027884998 12 0.1360990833287279 13 0.1315713251749346 14 0.11852072814341275 15 0.13523348250520859 16 0.1216502080438287 17 0.12551211941029944 18 0.11425930870454847 19 0.10007677213457829 20 0.09395098169121088 21 0.09395098169121088 22 0.10287332864133297 23 0.08376352584517598 24 0.09455024379980118 25 0.08789177592657575 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1501847.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.39730118300603 #Duplication Level Percentage of deduplicated Percentage of total 1 75.326617342397 40.22238073329019 2 14.367890734590743 15.344131778389276 3 4.620855109430055 7.402235760038067 4 1.892103045777787 4.041327848187182 5 0.9897696456332652 2.6425513934838305 6 0.6009133906497665 1.9252291983256165 7 0.4036019220664577 1.508587737343594 8 0.28389597579537923 1.2127423139351405 9 0.21619937917293333 1.039001702874844 >10 1.1448340158588988 10.987478718517362 >50 0.08182242374121136 3.0286529971370246 >100 0.06346937483805327 6.92007103936844 >500 0.0057698662849006346 2.0517806622784485 >1k 0.00225777376365677 1.673828116830995 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 3070 0.20441496370802087 No Hit TATCAACGCAGAGTACTTTTTTTTT 1877 0.12497944198044142 No Hit GCGCAAGACGGACCAGAGCGAAAGC 1853 0.1233814096908673 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.331693574645087E-4 2 0.0 0.0 0.0 0.0 1.331693574645087E-4 3 0.0 0.0 0.0 0.0 1.331693574645087E-4 4 0.0 0.0 0.0 0.0 1.331693574645087E-4 5 0.0 0.0 0.0 0.0 1.331693574645087E-4 6 0.0 0.0 0.0 0.0 1.331693574645087E-4 7 0.0 0.0 0.0 6.658467873225434E-5 1.331693574645087E-4 8 0.0 0.0 0.0 6.658467873225434E-5 1.331693574645087E-4 9 0.0 0.0 0.0 1.9975403619676305E-4 1.331693574645087E-4 10 0.0 0.0 0.0 1.9975403619676305E-4 1.331693574645087E-4 11 0.0 0.0 0.0 2.663387149290174E-4 1.331693574645087E-4 12 0.0 0.0 0.0 2.663387149290174E-4 8.656008235193066E-4 13 0.0 0.0 0.0 3.3292339366127176E-4 0.0012651088959128327 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATGCCG 25 0.006030389 19.000868 5 TGGACGT 50 8.710218E-5 15.200695 5 AACCGCG 75 1.4767937E-5 12.668089 7 TATTAGG 90 5.421243E-7 12.663443 2 GGTATCA 840 0.0 12.659643 1 ACGGTAT 230 0.0 12.3922825 9 CGAACGA 85 3.9425104E-6 12.294269 16 GTATTAG 125 1.4224497E-9 12.153258 1 CGCCGGT 260 0.0 12.059048 7 AGAACCG 95 1.0391068E-6 12.000547 5 CGTCGTA 240 0.0 11.876334 10 CGACCAT 340 0.0 11.736612 10 CCGACCA 325 0.0 11.693232 9 GTATCAA 2010 0.0 11.6204195 1 CTAGCAC 115 7.080234E-8 11.564202 3 CGGTCCA 290 0.0 11.466805 10 GTCCTAT 210 0.0 11.303253 1 CGTTATT 135 4.7439244E-9 11.256394 2 ATAATAC 85 5.3270982E-5 11.175489 3 CGCAAGA 290 0.0 11.135097 2 >>END_MODULE