FastQCFastQC Report
Thu 2 Feb 2017
SRR4062315_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062315_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1501847
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT17120.11399296998961944No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG407.0352945E-427.4984195
GTATCAA22750.026.1214311
TAGATCG551.5944905E-423.9986215
GGTATCA10850.022.9225711
ATACCGT3000.021.9987346
TACGAAT3150.021.65171241
CGCAATA3600.020.7786636
CGTCGTA3000.020.53215210
ATACGAA3450.020.4066640
ACCGTCG2850.019.2971368
TAGCGGC3700.019.02783230
CTAGCGG3950.018.93751129
TACCGTC3400.018.7636267
GTACGCT600.00741537618.332283
CAATACG3850.018.2864938
CGAATGC3750.018.18743743
GCGCAAT3900.018.05144735
CCGTGTA1355.5319106E-817.9248949
AACGCAG33350.017.7441066
TCTAGCG4000.017.60074628
Produced by FastQC (version 0.11.5)