##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062315_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1501847
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.250767221960693	32.0	32.0	32.0	32.0	32.0
2	31.462712912833332	32.0	32.0	32.0	32.0	32.0
3	31.570927664402564	32.0	32.0	32.0	32.0	32.0
4	31.65225951778044	32.0	32.0	32.0	32.0	32.0
5	31.627272285392586	32.0	32.0	32.0	32.0	32.0
6	35.26236627299585	36.0	36.0	36.0	36.0	36.0
7	35.2628889627239	36.0	36.0	36.0	36.0	36.0
8	35.21797027260433	36.0	36.0	36.0	36.0	36.0
9	35.2862242292324	36.0	36.0	36.0	36.0	36.0
10	35.176719732436126	36.0	36.0	36.0	36.0	36.0
11	35.28936702606857	36.0	36.0	36.0	36.0	36.0
12	35.21814938539012	36.0	36.0	36.0	36.0	36.0
13	35.258151462832096	36.0	36.0	36.0	36.0	36.0
14	35.20876827000354	36.0	36.0	36.0	36.0	36.0
15	35.194534463230944	36.0	36.0	36.0	36.0	36.0
16	35.20634725108483	36.0	36.0	36.0	36.0	36.0
17	35.18456340759079	36.0	36.0	36.0	36.0	36.0
18	35.1838589416898	36.0	36.0	36.0	36.0	36.0
19	35.173434444387475	36.0	36.0	36.0	36.0	36.0
20	35.17215668440261	36.0	36.0	36.0	36.0	36.0
21	35.16169623137377	36.0	36.0	36.0	36.0	36.0
22	35.15146216625262	36.0	36.0	36.0	36.0	36.0
23	35.12233869362192	36.0	36.0	36.0	36.0	36.0
24	35.10311969195264	36.0	36.0	36.0	36.0	36.0
25	35.08590422326642	36.0	36.0	36.0	36.0	36.0
26	35.04571104779648	36.0	36.0	36.0	36.0	36.0
27	35.0349795951252	36.0	36.0	36.0	36.0	36.0
28	35.017662251880516	36.0	36.0	36.0	36.0	36.0
29	34.997661546082924	36.0	36.0	36.0	36.0	36.0
30	34.98056859320557	36.0	36.0	36.0	36.0	36.0
31	34.9843958805391	36.0	36.0	36.0	36.0	36.0
32	34.95565726735147	36.0	36.0	36.0	36.0	36.0
33	34.93805427583502	36.0	36.0	36.0	36.0	36.0
34	34.920238213346636	36.0	36.0	36.0	36.0	36.0
35	34.908516646502605	36.0	36.0	36.0	36.0	36.0
36	34.88911853204754	36.0	36.0	36.0	32.0	36.0
37	34.882490027279744	36.0	36.0	36.0	32.0	36.0
38	34.86514205508284	36.0	36.0	36.0	32.0	36.0
39	34.8536155813475	36.0	36.0	36.0	32.0	36.0
40	34.8347128569022	36.0	36.0	36.0	32.0	36.0
41	34.80772075983772	36.0	36.0	36.0	32.0	36.0
42	34.778681849749006	36.0	36.0	36.0	32.0	36.0
43	34.76837787071519	36.0	36.0	36.0	32.0	36.0
44	34.72553728841886	36.0	36.0	36.0	32.0	36.0
45	34.69995612069672	36.0	36.0	36.0	32.0	36.0
46	34.69000304291982	36.0	36.0	36.0	32.0	36.0
47	34.65731862167052	36.0	36.0	36.0	32.0	36.0
48	34.63487359231666	36.0	36.0	36.0	32.0	36.0
49	34.6164256412271	36.0	36.0	36.0	32.0	36.0
50	34.212877210528106	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	8.0
20	15.0
21	64.0
22	175.0
23	421.0
24	1038.0
25	2168.0
26	4193.0
27	7800.0
28	12721.0
29	19790.0
30	27870.0
31	40474.0
32	60559.0
33	101504.0
34	231058.0
35	991989.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.58151276176267	17.447447647466298	11.731496776256195	26.239542814514838
2	16.542768752155784	19.62199372209134	36.72143744664643	27.113800079106444
3	19.237947609564007	22.6666107352386	28.01192404778408	30.083517607413313
4	12.907772895641168	15.535204318415923	34.68489133713354	36.87213144880936
5	14.90264987045951	36.28831698568496	32.77997026328248	16.029062880573054
6	35.49207807729825	34.31858813659199	16.137817151570236	14.051516634539515
7	31.30598523018656	29.8537733870361	19.453512907772897	19.386728475004446
8	28.640866879249348	32.79009113444978	18.600296834497787	19.96874515180308
9	27.094237961656546	14.435891272546405	18.36199026931505	40.107880496482004
10	16.80207797880472	26.334026703172896	30.45003342557549	26.41386189244688
11	37.58718431371504	21.164273058440706	21.498328391640424	19.75021423620382
12	24.800728702724044	23.418697110957375	27.923550135266773	23.857024051051805
13	29.541757582496754	19.186774684771486	24.898874519175386	26.37259321355637
14	23.837914248255647	19.22539379843619	24.65843724427322	32.27825470903494
15	25.458651913277453	26.616492891752625	21.954832949028763	25.970022245941166
16	26.180971944321445	25.712877912686814	23.0733735729957	25.03277656999604
17	24.426522808248777	25.83605387233187	24.568880851378335	25.168542468041018
18	25.06275626863545	24.87252320816704	25.545763136202076	24.518957386995435
19	25.733979559835323	24.748792653312886	25.18565473047521	24.33157305637658
20	26.067921925209177	23.959644223560133	24.84748728561327	25.124946565617424
21	26.69326502633091	23.96855338792833	24.593650351866735	24.744531233874024
22	26.238824593983274	23.855159680047304	24.99402402508378	24.911991700885643
23	24.765604972550427	24.012664422760004	25.38391112265247	25.837819482037094
24	24.854710392934475	24.512460814420827	25.34045176944364	25.292377023201055
25	25.14300819596268	23.98649121043096	25.264126533039207	25.606374060567163
26	24.86161947002238	25.068166518736724	25.362006662507312	24.708207348733584
27	25.367598833698107	24.480554199812097	25.048257438703565	25.10358952778623
28	25.082483198730078	24.263410885851243	25.439449841226086	25.214656074192597
29	24.84951792467906	24.663878975123847	25.502250572630906	24.984352527566188
30	24.926822512298347	24.70722346367227	25.470559877695713	24.89539414633367
31	25.26980237575241	24.56147126191871	24.75097214084057	25.41775422148831
32	25.256252671726244	24.497981806293357	24.737155405576367	25.508610116404036
33	24.80347466644294	24.256812634085342	25.21350448316134	25.72620821631037
34	25.518517063504483	24.49017232964295	25.21189135129053	24.779419255562043
35	25.830048473872054	24.148910669578648	25.338451499493953	24.682589357055345
36	24.708674220506698	24.82126936206962	25.087342801268576	25.382713616155105
37	25.69688963228518	24.553806857830786	25.01205207562045	24.737251434263584
38	24.98962943628745	24.348418980095843	25.20296674694559	25.45898483667111
39	25.637686138832738	24.42310611811143	24.67126903336982	25.26793870968601
40	25.658889127114232	24.500514699909314	25.02067455651245	24.819921616464004
41	24.683752488630535	24.981922055092788	25.423916155623473	24.910409300653203
42	25.807244222840158	24.73870936810816	25.023905029778078	24.430141379273607
43	25.559424331470858	24.017640552762177	25.067137578599073	25.355797537167884
44	24.952223297984123	24.753658663264478	24.98471811412872	25.30939992462268
45	25.2230146749882	24.8214317818468	24.87503387585755	25.08051966730745
46	25.06289094596772	24.680351523432453	24.92465737918213	25.33210015141769
47	25.198857923620977	24.5620331516434	25.178016627758478	25.061092296977144
48	25.61358813302339	25.09866618368457	24.42586502292944	24.861880660362594
49	25.0671535549101	25.118226213924522	24.518338880077135	25.29628135108824
50	25.312387220323046	25.201255541602137	24.497377185844925	24.98898005222989
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	249.0
1	161.0
2	73.0
3	199.5
4	326.0
5	330.0
6	334.0
7	282.5
8	231.0
9	332.0
10	433.0
11	706.5
12	980.0
13	1684.5
14	2389.0
15	3064.5
16	3740.0
17	3660.0
18	3580.0
19	3317.0
20	3054.0
21	3467.5
22	3881.0
23	4349.0
24	4817.0
25	5933.5
26	7050.0
27	8903.5
28	10757.0
29	12856.0
30	14955.0
31	17312.0
32	19669.0
33	22793.5
34	25918.0
35	30236.0
36	34554.0
37	41130.0
38	47706.0
39	53846.0
40	59986.0
41	66844.0
42	73702.0
43	78415.5
44	83129.0
45	90883.5
46	98638.0
47	104657.0
48	110676.0
49	115566.5
50	120457.0
51	118826.5
52	117196.0
53	116713.5
54	116231.0
55	116626.0
56	117021.0
57	112766.5
58	108512.0
59	99457.5
60	90403.0
61	79412.5
62	68422.0
63	59008.5
64	49595.0
65	41776.5
66	33958.0
67	28892.5
68	23827.0
69	20691.5
70	17556.0
71	13790.5
72	10025.0
73	8669.0
74	7313.0
75	5440.5
76	3568.0
77	3110.5
78	2653.0
79	2257.5
80	1862.0
81	1457.5
82	1053.0
83	887.0
84	721.0
85	565.0
86	409.0
87	277.5
88	146.0
89	94.5
90	43.0
91	29.0
92	15.0
93	11.5
94	8.0
95	9.0
96	10.0
97	9.5
98	9.0
99	8.0
100	7.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03242673854260787
2	0.004860681547454567
3	5.326774298580349E-4
4	0.0
5	0.0
6	1.3316935746450872E-4
7	0.0
8	0.0
9	0.0
10	1.9975403619676307E-4
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	7.990161447870523E-4
17	0.0
18	3.3292339366127176E-4
19	0.0
20	3.3292339366127176E-4
21	0.0
22	0.0
23	1.3316935746450872E-4
24	0.0012651088959128327
25	6.658467873225435E-4
26	6.658467873225435E-4
27	3.9950807239352615E-4
28	0.0017312016470386131
29	4.660927511257805E-4
30	0.0012651088959128327
31	4.660927511257805E-4
32	5.992621085902891E-4
33	3.3292339366127176E-4
34	0.0010653548597160697
35	4.660927511257805E-4
36	3.9950807239352615E-4
37	0.002064125040699885
38	0.0
39	7.324314660547978E-4
40	6.658467873225436E-5
41	0.001131939538448324
42	0.004727512189990059
43	0.005326774298580348
44	0.004594342832525551
45	0.002663387149290174
46	0.0027299718280224283
47	0.0013982782533773414
48	0.004394588796328787
49	0.004328004117596532
50	0.0016646169683063585
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1501847.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.81662843677137
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.03884790152486	41.133766437412895
2	14.84286544616217	16.272716801985286
3	4.572528513181767	7.519517895708826
4	1.8968563208423876	4.159170725502333
5	0.9631918121188376	2.639946383912941
6	0.5845044174101328	1.9224336881293658
7	0.38683671905403544	1.4843559285859342
8	0.27827697598608897	1.2203364476110232
9	0.2051638344877921	1.0121750715402504
>10	1.0924617956702483	10.552415354013188
>50	0.07628327328667849	2.9219276128604585
>100	0.055708252218369024	6.197159245231939
>500	0.004642265021839977	1.7043015559852128
>1k	0.001832473034936833	1.259776851520386
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	1712	0.11399296998961944	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	6.658467873225434E-5
2	0.0	0.0	0.0	0.0	6.658467873225434E-5
3	0.0	0.0	0.0	0.0	6.658467873225434E-5
4	0.0	0.0	0.0	0.0	6.658467873225434E-5
5	0.0	0.0	0.0	0.0	6.658467873225434E-5
6	0.0	0.0	0.0	0.0	1.9975403619676305E-4
7	0.0	0.0	0.0	0.0	1.9975403619676305E-4
8	0.0	0.0	0.0	0.0	1.9975403619676305E-4
9	0.0	0.0	0.0	0.0	1.9975403619676305E-4
10	0.0	0.0	0.0	0.0	1.9975403619676305E-4
11	0.0	0.0	0.0	0.0	1.9975403619676305E-4
12	0.0	0.0	0.0	0.0	5.992621085902891E-4
13	0.0	0.0	0.0	0.0	6.658467873225435E-4
14	0.0	0.0	0.0	0.0	6.658467873225435E-4
15	0.0	0.0	0.0	0.0	7.990161447870522E-4
16	0.0	0.0	0.0	0.0	7.990161447870522E-4
17	0.0	0.0	0.0	0.0	7.990161447870522E-4
18	0.0	0.0	0.0	0.0	0.0010653548597160695
19	0.0	0.0	0.0	6.658467873225434E-5	0.001131939538448324
20	0.0	0.0	0.0	1.9975403619676305E-4	0.001331693574645087
21	0.0	0.0	0.0	2.663387149290174E-4	0.0013982782533773414
22	0.0	0.0	0.0	3.995080723935261E-4	0.0015980322895741044
23	0.0	0.0	0.0	7.324314660547979E-4	0.0015980322895741044
24	0.0	0.0	0.0	0.001331693574645087	0.0015980322895741044
25	6.658467873225434E-5	0.0	0.0	0.0015980322895741044	0.0015980322895741044
26	6.658467873225434E-5	0.0	0.0	0.0019975403619676307	0.0017977863257708675
27	6.658467873225434E-5	0.0	0.0	0.0023304637556289024	0.0017977863257708675
28	6.658467873225434E-5	0.0	0.0	0.0040616654026675155	0.001864371004503122
29	6.658467873225434E-5	0.0	0.0	0.006525298515760927	0.001864371004503122
30	6.658467873225434E-5	0.0	0.0	0.011718903456876767	0.001864371004503122
31	6.658467873225434E-5	0.0	0.0	0.022838544805163244	0.001864371004503122
32	6.658467873225434E-5	0.0	0.0	0.03389160147471747	0.001930955683235376
33	6.658467873225434E-5	0.0	0.0	0.04567708961032649	0.0019975403619676307
34	6.658467873225434E-5	0.0	0.0	0.056064299492558166	0.002064125040699885
35	6.658467873225434E-5	0.0	0.0	0.0704465900987251	0.002064125040699885
36	6.658467873225434E-5	0.0	0.0	0.0886907920713628	0.002064125040699885
37	6.658467873225434E-5	0.0	0.0	0.11539124824299679	0.002064125040699885
38	6.658467873225434E-5	0.0	0.0	0.153144761084185	0.002064125040699885
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATACCG	40	7.0352945E-4	27.498419	5
GTATCAA	2275	0.0	26.121431	1
TAGATCG	55	1.5944905E-4	23.998621	5
GGTATCA	1085	0.0	22.922571	1
ATACCGT	300	0.0	21.998734	6
TACGAAT	315	0.0	21.651712	41
CGCAATA	360	0.0	20.77866	36
CGTCGTA	300	0.0	20.532152	10
ATACGAA	345	0.0	20.40666	40
ACCGTCG	285	0.0	19.297136	8
TAGCGGC	370	0.0	19.027832	30
CTAGCGG	395	0.0	18.937511	29
TACCGTC	340	0.0	18.763626	7
GTACGCT	60	0.007415376	18.33228	3
CAATACG	385	0.0	18.28649	38
CGAATGC	375	0.0	18.187437	43
GCGCAAT	390	0.0	18.051447	35
CCGTGTA	135	5.5319106E-8	17.924894	9
AACGCAG	3335	0.0	17.744106	6
TCTAGCG	400	0.0	17.600746	28
>>END_MODULE