FastQCFastQC Report
Thu 2 Feb 2017
SRR4062314_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062314_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1404799
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT26910.1915576534436599No Hit
TATCAACGCAGAGTACTTTTTTTTT17970.12791865597854213No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTAACG400.00529336914.2440662
CGAACGA500.001500099213.29920116
GGTATCA6000.013.1329191
GTATAAT957.4491254E-812.9913471
ACGGTAT1550.012.8734079
TAATACT1251.4078978E-912.160574
ATAATAC1103.821151E-812.088893
GTACTAT802.8854902E-511.8670961
GTATCAA14900.011.8511671
CGGTCCA1251.8255378E-811.4021610
GACGGTA1751.8189894E-1211.4017548
GTCTTAC752.0864043E-411.3924111
TAGACTG1201.2725104E-711.0846435
TAGGACG600.00587312311.0838544
GGGTCGC951.3569677E-511.0020866
GTGTATA1951.8189894E-1210.71081351
CGCGTAA803.7635834E-410.68952610
CGCATCG1605.4387783E-1010.68800213
ATCGCCA1605.4387783E-1010.68685816
GTAGGAC1701.382432E-1010.6158743
Produced by FastQC (version 0.11.5)