##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062314_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1404799
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.170543971059203	32.0	32.0	32.0	32.0	32.0
2	30.740548647884857	32.0	32.0	32.0	32.0	32.0
3	30.763645190521917	32.0	32.0	32.0	32.0	32.0
4	30.766519623091988	32.0	32.0	32.0	32.0	32.0
5	30.642109654121338	32.0	32.0	32.0	32.0	32.0
6	34.299785236179694	36.0	36.0	36.0	32.0	36.0
7	34.232127158404865	36.0	36.0	36.0	32.0	36.0
8	34.17976308354434	36.0	36.0	36.0	32.0	36.0
9	34.324127508632905	36.0	36.0	36.0	32.0	36.0
10	33.97356917252931	36.0	36.0	36.0	32.0	36.0
11	34.32396947890766	36.0	36.0	36.0	32.0	36.0
12	34.11724239553132	36.0	36.0	36.0	32.0	36.0
13	34.2167662420033	36.0	36.0	36.0	32.0	36.0
14	34.099331648157495	36.0	36.0	36.0	32.0	36.0
15	34.03774134235574	36.0	36.0	36.0	32.0	36.0
16	34.044804986336125	36.0	36.0	36.0	32.0	36.0
17	33.98218819916586	36.0	36.0	36.0	32.0	36.0
18	33.97664221002435	36.0	36.0	36.0	32.0	36.0
19	33.989812777486314	36.0	36.0	36.0	32.0	36.0
20	33.976578143919525	36.0	36.0	36.0	32.0	36.0
21	33.95679524259342	36.0	36.0	36.0	32.0	36.0
22	33.928960655581335	36.0	36.0	36.0	32.0	36.0
23	33.88208491036796	36.0	36.0	36.0	32.0	36.0
24	33.860711034105236	36.0	36.0	36.0	32.0	36.0
25	33.50138631932398	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	1.0
4	28.0
5	93.0
6	303.0
7	70.0
8	227.0
9	196.0
10	86.0
11	28.0
12	64.0
13	50.0
14	214.0
15	309.0
16	529.0
17	627.0
18	894.0
19	1184.0
20	1797.0
21	2683.0
22	3949.0
23	6110.0
24	8452.0
25	12270.0
26	17025.0
27	22449.0
28	30160.0
29	40474.0
30	53620.0
31	72842.0
32	103114.0
33	142397.0
34	297519.0
35	585035.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.611469411580465	17.399165705662096	12.031634846737568	25.95773003601988
2	16.5851123655493	19.336769196819453	37.3608154955545	26.717302942076753
3	19.123485272898247	22.67461850196933	28.370127656000072	29.83176856913235
4	13.111413914031496	15.369407697553989	34.970320093062064	36.548858295352446
5	15.020290858183316	36.14243691106132	33.34607965238865	15.49119257836672
6	34.90966462122534	34.54875047633972	16.421629206480358	14.119955695954584
7	30.726798397228283	30.145533552955527	20.039919647635358	19.08774840218083
8	28.17275577050935	33.131926980636344	19.04968221312778	19.645635035726528
9	26.959289426853456	14.605718993492495	18.651190999672878	39.78380057998117
10	16.497192376721888	26.692735248038797	31.07480865537725	25.73526371986206
11	36.84777773976118	21.51442444771387	21.855718456678186	19.78207935584676
12	24.20121755208025	23.613895860166117	28.595866367208632	23.589020220545
13	29.31329156777638	19.557399131903612	25.0844220174332	26.044887282886815
14	23.472097309146676	19.33861080189681	24.98065134072312	32.20864054823339
15	25.07419856991344	27.099014106994197	22.130418420062266	25.6963689030301
16	25.708460042376114	25.93053196773828	23.48959376374141	24.871414226144196
17	24.100408450091905	26.145652262226243	24.98679717684086	24.767142110840997
18	24.662447452829582	25.393814274302105	25.723545764407667	24.220192508460638
19	25.4222047416895	25.26865631230112	25.252482069391064	24.056656876618316
20	25.652569809793608	24.633352523070435	24.9644467370797	24.749630930056256
21	26.10316696947027	24.50963627943429	24.759930177051046	24.627266574044388
22	25.679313553778606	24.316624887238298	25.046565417464134	24.957496141518966
23	24.582208322350375	24.460813997392584	25.397773012559753	25.559204667697283
24	24.633098446038858	24.89430071748687	25.305484107701403	25.167116728772864
25	24.886896203844746	24.54063828301669	25.29385613054636	25.278609382592204
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	353.0
1	353.0
2	415.5
3	478.0
4	478.0
5	478.0
6	980.5
7	1483.0
8	1483.0
9	1483.0
10	1817.5
11	2152.0
12	2152.0
13	2152.0
14	2965.5
15	3779.0
16	3779.0
17	3779.0
18	6369.5
19	8960.0
20	8960.0
21	8960.0
22	13946.5
23	18933.0
24	18933.0
25	18933.0
26	27470.5
27	36008.0
28	36008.0
29	36008.0
30	45878.5
31	55749.0
32	55749.0
33	55749.0
34	69682.0
35	83615.0
36	83615.0
37	83615.0
38	99381.5
39	115148.0
40	115148.0
41	115148.0
42	132586.0
43	150024.0
44	150024.0
45	150024.0
46	165979.0
47	181934.0
48	181934.0
49	181934.0
50	188030.0
51	194126.0
52	194126.0
53	194126.0
54	182886.5
55	171647.0
56	171647.0
57	171647.0
58	156804.5
59	141962.0
60	141962.0
61	141962.0
62	123983.5
63	106005.0
64	106005.0
65	106005.0
66	86062.0
67	66119.0
68	66119.0
69	66119.0
70	49965.5
71	33812.0
72	33812.0
73	33812.0
74	25383.0
75	16954.0
76	16954.0
77	16954.0
78	13044.0
79	9134.0
80	9134.0
81	9134.0
82	6347.5
83	3561.0
84	3561.0
85	3561.0
86	2591.5
87	1622.0
88	1622.0
89	1622.0
90	1088.5
91	555.0
92	555.0
93	555.0
94	345.5
95	136.0
96	136.0
97	136.0
98	343.0
99	550.0
100	550.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0013525066575360604
2	6.406610483065549E-4
3	5.694764873836044E-4
4	0.010321761333827829
5	0.033599112755632654
6	0.061503460637429265
7	0.10535315016596682
8	0.10677684138442581
9	0.1174545255228684
10	0.13048130017176834
11	0.1354642194363749
12	0.12955590087976998
13	0.12307810583578149
14	0.11396648203764381
15	0.12863050158777165
16	0.1174545255228684
17	0.12030190795978642
18	0.10933948557765204
19	0.09488901971029308
20	0.09047557693307014
21	0.08890951659276523
22	0.09944483160936192
23	0.07894367806355215
24	0.09175689902968324
25	0.08691634888692261
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1404799.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.7367613863941
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.86297658678113	44.955561000253724
2	13.0106086493254	15.023808141557115
3	3.8760876958883292	6.713782512307278
4	1.6720087187136679	3.8614547371336627
5	0.9075108522635784	2.6198368766352687
6	0.562966459711004	1.9502316091726373
7	0.39459813693344403	1.5947972932949344
8	0.27740750010010645	1.2813288512060756
9	0.20845483456687158	1.0831956338904947
>10	1.1118794661672948	11.297731555618372
>50	0.0667643383997126	2.66818462923474
>100	0.04440739690555853	5.0207976777219265
>500	0.003958275880191675	1.5297050201523272
>1k	3.710883637679695E-4	0.39958446182138624
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	2691	0.1915576534436599	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1797	0.12791865597854213	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	7.118456092295055E-5	0.0
7	0.0	0.0	0.0	7.118456092295055E-5	0.0
8	0.0	0.0	0.0	7.118456092295055E-5	0.0
9	0.0	0.0	0.0	7.118456092295055E-5	0.0
10	0.0	0.0	0.0	7.118456092295055E-5	7.118456092295055E-5
11	0.0	0.0	0.0	7.118456092295055E-5	7.118456092295055E-5
12	0.0	0.0	0.0	7.118456092295055E-5	2.847382436918022E-4
13	0.0	0.0	0.0	7.118456092295055E-5	2.847382436918022E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTAACG	40	0.005293369	14.244066	2
CGAACGA	50	0.0015000992	13.299201	16
GGTATCA	600	0.0	13.132919	1
GTATAAT	95	7.4491254E-8	12.991347	1
ACGGTAT	155	0.0	12.873407	9
TAATACT	125	1.4078978E-9	12.16057	4
ATAATAC	110	3.821151E-8	12.08889	3
GTACTAT	80	2.8854902E-5	11.867096	1
GTATCAA	1490	0.0	11.851167	1
CGGTCCA	125	1.8255378E-8	11.40216	10
GACGGTA	175	1.8189894E-12	11.401754	8
GTCTTAC	75	2.0864043E-4	11.392411	1
TAGACTG	120	1.2725104E-7	11.084643	5
TAGGACG	60	0.005873123	11.083854	4
GGGTCGC	95	1.3569677E-5	11.002086	6
GTGTATA	195	1.8189894E-12	10.7108135	1
CGCGTAA	80	3.7635834E-4	10.689526	10
CGCATCG	160	5.4387783E-10	10.688002	13
ATCGCCA	160	5.4387783E-10	10.686858	16
GTAGGAC	170	1.382432E-10	10.615874	3
>>END_MODULE