FastQCFastQC Report
Thu 2 Feb 2017
SRR4062314_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062314_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1404799
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA7350.026.9470371
GTATCAA16450.026.3545861
CGTCTTA500.0025808521.99969915
ATTATAC1201.4320904E-820.1663883
ACCGTAT600.007413274618.333088
TCAACGC24250.017.4183164
ATCAACG24700.017.2791143
TATCAAC25800.016.9693622
CAACGCA24900.016.9636235
TCTAGCG2650.016.60354428
TACACCG800.00198874916.4997735
AACGCAG25900.016.2237156
GGCGTTA3500.015.71462942
CTAGCGG2800.015.71406929
TCTACAC3650.015.6710173
CGAGCCG2950.015.66080215
TCCGTAC850.002980535815.5291983
TACGAAT2850.015.43893341
CGCAATA3050.015.14787236
TAAGACT2203.0559022E-1014.9997944
Produced by FastQC (version 0.11.5)