##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062314_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1404799
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.953615428256995	32.0	32.0	32.0	32.0	32.0
2	31.284467742360295	32.0	32.0	32.0	32.0	32.0
3	31.470453780220517	32.0	32.0	32.0	32.0	32.0
4	31.585515792650764	32.0	32.0	32.0	32.0	32.0
5	31.515564148322998	32.0	32.0	32.0	32.0	32.0
6	35.18118819845402	36.0	36.0	36.0	36.0	36.0
7	35.18858142695147	36.0	36.0	36.0	36.0	36.0
8	35.12686868370493	36.0	36.0	36.0	36.0	36.0
9	35.24518667795179	36.0	36.0	36.0	36.0	36.0
10	35.083921614408894	36.0	36.0	36.0	36.0	36.0
11	35.26195633681402	36.0	36.0	36.0	36.0	36.0
12	35.15534037253728	36.0	36.0	36.0	36.0	36.0
13	35.21086860113084	36.0	36.0	36.0	36.0	36.0
14	35.16488764584827	36.0	36.0	36.0	36.0	36.0
15	35.13571763647326	36.0	36.0	36.0	36.0	36.0
16	35.15490614671565	36.0	36.0	36.0	36.0	36.0
17	35.13208935940302	36.0	36.0	36.0	36.0	36.0
18	35.1280005182236	36.0	36.0	36.0	36.0	36.0
19	35.11731785116589	36.0	36.0	36.0	36.0	36.0
20	35.12630205459998	36.0	36.0	36.0	36.0	36.0
21	35.11872659362656	36.0	36.0	36.0	36.0	36.0
22	35.10128139328118	36.0	36.0	36.0	36.0	36.0
23	35.06246658774672	36.0	36.0	36.0	36.0	36.0
24	35.046657208611336	36.0	36.0	36.0	36.0	36.0
25	35.03476084478989	36.0	36.0	36.0	36.0	36.0
26	34.99615816924699	36.0	36.0	36.0	36.0	36.0
27	34.981553944728034	36.0	36.0	36.0	36.0	36.0
28	34.963141346199706	36.0	36.0	36.0	36.0	36.0
29	34.9459880025541	36.0	36.0	36.0	36.0	36.0
30	34.91867448652797	36.0	36.0	36.0	32.0	36.0
31	34.92995012097816	36.0	36.0	36.0	32.0	36.0
32	34.89569539841643	36.0	36.0	36.0	32.0	36.0
33	34.86866377325155	36.0	36.0	36.0	32.0	36.0
34	34.86271132026717	36.0	36.0	36.0	32.0	36.0
35	34.85321316430322	36.0	36.0	36.0	32.0	36.0
36	34.82109753779722	36.0	36.0	36.0	32.0	36.0
37	34.81776823588286	36.0	36.0	36.0	32.0	36.0
38	34.80039137271596	36.0	36.0	36.0	32.0	36.0
39	34.78572450578339	36.0	36.0	36.0	32.0	36.0
40	34.77126620961433	36.0	36.0	36.0	32.0	36.0
41	34.74336399726936	36.0	36.0	36.0	32.0	36.0
42	34.716170783151185	36.0	36.0	36.0	32.0	36.0
43	34.702917641598546	36.0	36.0	36.0	32.0	36.0
44	34.65555143476042	36.0	36.0	36.0	32.0	36.0
45	34.641035479097006	36.0	36.0	36.0	32.0	36.0
46	34.627181539850184	36.0	36.0	36.0	32.0	36.0
47	34.591033307967905	36.0	36.0	36.0	32.0	36.0
48	34.5680955069017	36.0	36.0	36.0	32.0	36.0
49	34.55884436136415	36.0	36.0	36.0	32.0	36.0
50	34.11936440729243	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	4.0
20	10.0
21	27.0
22	77.0
23	227.0
24	577.0
25	1426.0
26	3039.0
27	6025.0
28	10656.0
29	17557.0
30	27140.0
31	41817.0
32	65944.0
33	115491.0
34	259502.0
35	855280.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.92404598198773	18.328709690282373	12.467530063173966	26.27971426455593
2	16.115591579645756	19.920070561150503	36.95932126893323	27.005016590270504
3	18.531445135094348	23.150122864814534	28.625295060635136	29.69313693945598
4	12.950820722395163	15.83023621172851	35.06864683132604	36.15029623455028
5	14.86952937751237	36.32434248600689	33.10708507053322	15.699043065947512
6	35.14199580721743	34.790343074968234	16.18713050658637	13.880530611227973
7	30.772160287699524	30.158549372543686	20.064792187352072	19.00449815240472
8	28.237562811476945	33.20119106007336	18.90434147518613	19.656904653263563
9	27.121317711644156	14.396507970179364	18.545215365329845	39.93695895284664
10	16.363016409500027	26.729646155028913	31.220333770882036	25.68700366458902
11	37.1301517156547	21.25236421722965	21.92975649897245	19.687727568143202
12	24.317856148815594	23.46819722963926	28.59220429399508	23.621742327550063
13	29.421006136821	19.52919955096779	25.181324872810983	25.868469439400226
14	23.554757655721566	19.15071124054046	25.04336919374231	32.25116190999566
15	25.212646079617084	27.09654548444297	21.96591825592131	25.724890180018633
16	25.757283467789872	25.893317938397953	23.489330441325436	24.86006815248674
17	24.166375403171557	26.087504333360144	24.995817907045776	24.75030235642252
18	24.850832646401834	25.281997838835192	25.704976800935082	24.162192713827896
19	25.540735720910963	25.127865267557848	25.27329532552344	24.058103686007748
20	25.698322033946564	24.49330721328933	24.991244280308315	24.817126472455787
21	26.280983969948725	24.348038402646928	24.79564692173044	24.57533070567391
22	25.71698869375619	24.228021232930832	25.11241821783757	24.94257185547541
23	24.667407461718668	24.36344895383461	25.431645995827157	25.53749758861957
24	24.687249518434733	24.70355084090621	25.36692212523687	25.24227751542219
25	24.958623915056766	24.42046786831122	25.42388686763995	25.19702134899206
26	24.70632704866336	25.011069238621463	25.63237029770913	24.650233415006042
27	25.13382726032819	24.78779837072429	25.094675668210904	24.98369870073662
28	24.933370349805664	24.605916940730932	25.469610899927392	24.991101809536012
29	24.888506387792045	24.842876759427927	25.360890496722284	24.90772635605774
30	24.818085653147605	24.95824974978324	25.336529084228015	24.887135512841137
31	25.206257999393504	24.628094937763116	24.971419297064195	25.194227765779182
32	25.102222120980876	24.79648116721147	24.83008063873165	25.271216073076
33	24.781124648080326	24.56472296669621	25.258845596688484	25.39530678853498
34	25.282676909759793	24.604138429822665	25.364611214250733	24.748573446166812
35	25.504113417421642	24.619855024904027	25.253400323036917	24.622631234637417
36	24.848073701961784	24.97827081997134	25.05023871844464	25.123416759622234
37	25.521938204566748	24.67190218428149	25.065776173079605	24.74038343807216
38	25.016674983396204	24.604018083725858	25.22175770341522	25.157549229462724
39	25.40146712842179	24.601131133945763	24.939901835512195	25.057499902120256
40	25.503079088182723	24.800558656434124	25.09633050706898	24.600031748314173
41	24.826610724615193	24.938656180616707	25.39616326176139	24.838569833006712
42	25.621237284986442	24.92801143559257	24.97905301846538	24.47169826095561
43	25.11331334851534	24.358080859494297	25.279964235196296	25.248641556794066
44	24.864406574545953	24.811728199517212	25.083520139868202	25.240345086068633
45	25.042943309137012	25.042302629846112	24.89281079530368	25.021943265713194
46	24.919879438308946	24.839296163467303	24.941591363067516	25.29923303515623
47	25.03457834352828	24.89014301873388	25.20421332577818	24.871065311959658
48	25.507705274667696	25.145078989031518	24.515644042507148	24.831571693793638
49	25.04378009436568	25.124933440019138	24.728990537804645	25.10229592781054
50	25.190296836726635	25.262123081281825	24.671709927326646	24.8758701546649
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	143.0
1	95.5
2	48.0
3	134.0
4	220.0
5	233.0
6	246.0
7	198.0
8	150.0
9	206.0
10	262.0
11	451.5
12	641.0
13	1202.5
14	1764.0
15	2220.5
16	2677.0
17	2669.5
18	2662.0
19	2580.0
20	2498.0
21	2989.0
22	3480.0
23	4433.0
24	5386.0
25	6794.0
26	8202.0
27	10171.5
28	12141.0
29	14127.5
30	16114.0
31	18747.5
32	21381.0
33	24958.0
34	28535.0
35	33305.5
36	38076.0
37	43687.5
38	49299.0
39	54694.0
40	60089.0
41	65797.0
42	71505.0
43	75363.5
44	79222.0
45	85933.5
46	92645.0
47	97857.5
48	103070.0
49	106628.0
50	110186.0
51	109396.5
52	108607.0
53	107403.0
54	106199.0
55	105214.5
56	104230.0
57	99833.0
58	95436.0
59	87789.5
60	80143.0
61	70700.5
62	61258.0
63	53099.0
64	44940.0
65	37740.5
66	30541.0
67	26032.5
68	21524.0
69	18763.5
70	16003.0
71	12580.0
72	9157.0
73	7930.5
74	6704.0
75	5043.0
76	3382.0
77	2894.5
78	2407.0
79	1994.0
80	1581.0
81	1273.0
82	965.0
83	788.5
84	612.0
85	432.5
86	253.0
87	181.0
88	109.0
89	70.0
90	31.0
91	25.5
92	20.0
93	18.0
94	16.0
95	13.5
96	11.0
97	15.0
98	19.0
99	14.0
100	9.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03011106927040808
2	0.004271073655377032
3	2.1355368276885164E-4
4	0.0
5	0.0
6	2.847382436918022E-4
7	0.0
8	0.0
9	0.0
10	2.1355368276885164E-4
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	5.694764873836044E-4
17	0.0
18	7.118456092295055E-5
19	0.0
20	2.1355368276885164E-4
21	0.0
22	0.0
23	1.423691218459011E-4
24	3.5592280461475275E-4
25	1.423691218459011E-4
26	3.5592280461475275E-4
27	2.1355368276885164E-4
28	0.0013525066575360604
29	7.118456092295055E-4
30	0.0012101375356901591
31	3.5592280461475275E-4
32	0.001067768413844258
33	2.847382436918022E-4
34	0.001067768413844258
35	4.2710736553770327E-4
36	4.2710736553770327E-4
37	0.002491459632303269
38	0.0
39	9.965838529213077E-4
40	0.0
41	8.542147310754065E-4
42	0.004413442777222934
43	0.004698181020914736
44	0.003416858924301626
45	0.002847382436918022
46	0.0029185669978409722
47	0.0012813220966131098
48	0.0033456743633786755
49	0.0036304126070704776
50	0.001423691218459011
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1404799.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.249676842455465
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.63274765824237	45.99715211142751
2	13.5114864028119	16.011024060556725
3	3.85329226332111	6.8491896414392865
4	1.5738112343004904	3.7299122817332013
5	0.8880682482959704	2.6308878362790873
6	0.5502194268454551	1.9560193939820572
7	0.38017088040434327	1.5767501266208388
8	0.2676386829841522	1.2686004381881122
9	0.19974269072855821	1.065122088957864
>10	1.0450220066203164	10.923174414154747
>50	0.05697726593172865	2.3300826133147603
>100	0.037924587059529544	4.459088284038891
>500	0.0027778752685241543	1.1266420909718682
>1k	1.207771855880067E-4	0.07635461833504721
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	2.1355368276885164E-4
6	0.0	0.0	0.0	0.0	2.1355368276885164E-4
7	0.0	0.0	0.0	0.0	2.1355368276885164E-4
8	0.0	0.0	0.0	0.0	2.1355368276885164E-4
9	0.0	0.0	0.0	0.0	2.1355368276885164E-4
10	0.0	0.0	0.0	0.0	2.1355368276885164E-4
11	0.0	0.0	0.0	0.0	2.847382436918022E-4
12	0.0	0.0	0.0	0.0	4.2710736553770327E-4
13	0.0	0.0	0.0	0.0	4.982919264606538E-4
14	0.0	0.0	0.0	0.0	4.982919264606538E-4
15	0.0	0.0	0.0	0.0	6.406610483065549E-4
16	0.0	0.0	0.0	0.0	6.406610483065549E-4
17	0.0	0.0	0.0	0.0	6.406610483065549E-4
18	0.0	0.0	0.0	0.0	6.406610483065549E-4
19	0.0	0.0	0.0	7.118456092295055E-5	6.406610483065549E-4
20	0.0	0.0	0.0	7.118456092295055E-5	6.406610483065549E-4
21	0.0	0.0	0.0	7.118456092295055E-5	6.406610483065549E-4
22	0.0	0.0	0.0	2.847382436918022E-4	6.406610483065549E-4
23	0.0	0.0	0.0	2.847382436918022E-4	7.118456092295054E-4
24	0.0	0.0	0.0	7.83030170152456E-4	7.118456092295054E-4
25	0.0	0.0	0.0	0.001067768413844258	7.118456092295054E-4
26	0.0	0.0	0.0	0.0013525066575360604	7.118456092295054E-4
27	0.0	0.0	0.0	0.0019219831449196647	7.118456092295054E-4
28	0.0	0.0	0.0	0.003132120680609824	7.118456092295054E-4
29	0.0	0.0	0.0	0.005979503117527846	7.118456092295054E-4
30	0.0	0.0	0.0	0.009467546602752423	7.118456092295054E-4
31	0.0	0.0	0.0	0.01843680127904419	8.542147310754065E-4
32	0.0	0.0	0.0	0.025412888249493344	9.965838529213077E-4
33	0.0	0.0	0.0	0.033527928194709705	9.965838529213077E-4
34	0.0	0.0	0.0	0.04199889094454082	0.001067768413844258
35	0.0	0.0	0.0	0.053317236131289955	0.001067768413844258
36	0.0	0.0	0.0	0.06705585638941941	0.001067768413844258
37	0.0	0.0	0.0	0.08613331871677016	0.001067768413844258
38	0.0	0.0	0.0	0.1106919922351881	0.001067768413844258
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	735	0.0	26.947037	1
GTATCAA	1645	0.0	26.354586	1
CGTCTTA	50	0.00258085	21.999699	15
ATTATAC	120	1.4320904E-8	20.166388	3
ACCGTAT	60	0.0074132746	18.33308	8
TCAACGC	2425	0.0	17.418316	4
ATCAACG	2470	0.0	17.279114	3
TATCAAC	2580	0.0	16.969362	2
CAACGCA	2490	0.0	16.963623	5
TCTAGCG	265	0.0	16.603544	28
TACACCG	80	0.001988749	16.499773	5
AACGCAG	2590	0.0	16.223715	6
GGCGTTA	350	0.0	15.714629	42
CTAGCGG	280	0.0	15.714069	29
TCTACAC	365	0.0	15.671017	3
CGAGCCG	295	0.0	15.660802	15
TCCGTAC	85	0.0029805358	15.529198	3
TACGAAT	285	0.0	15.438933	41
CGCAATA	305	0.0	15.147872	36
TAAGACT	220	3.0559022E-10	14.999794	4
>>END_MODULE