FastQCFastQC Report
Thu 2 Feb 2017
SRR4062313_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062313_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5802551
Sequences flagged as poor quality0
Sequence length25
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAAGACGGACCAGAGCGAAAGC107540.18533227885459344No Hit
GGGTAGGCACACGCTGAGCCAGTCA84910.14633219078987844No Hit
GAATAGGACCGCGGTTCTATTTTGT78080.13456150579288317No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG74600.12856414359822085No Hit
GATTAAGAGGGACGGCCGGGGGCAT73240.12622034687846778No Hit
TCGTAGTTCCGACCATAAACGATGC73080.12594460608790858No Hit
ATCAGATACCGTCGTAGTTCCGACC70730.12189466322657051No Hit
GTGTAGCGCGCGTGCAGCCCCGGAC70670.12179126043011083No Hit
GTATCAACGCAGAGTACTTTTTTTT67550.11641431501420668No Hit
GAACTACGACGGTATCTGATCGTCT66520.11463923367498192No Hit
GTGCATGGCCGTTCTTAGTTGGTGG64270.11076162880774335No Hit
CTCCTGGTGGTGCCCTTCCGTCAAT61880.1066427507487655No Hit
GTCTTGCGCCGGTCCAAGAATTTCA60040.10347173165733486No Hit
GCCATGCACCACCACCCACGGAATC58570.10093836314407233No Hit
GAATAACGCCGCCGCATCGCCAGTC58460.10074879135056289No Hit
GTATCTGATCGTCTTCGAACCTCCG58260.1004041153623639No Hit
ACCATACTCCCCCCGGAACCCAAAG58120.10016284217062461No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25350.014.9847811
AAGACGG19400.012.9283475
CAAGACG20000.012.5872364
TCGCGTA8700.012.4500579
CGTTATT7150.012.2213072
CGCATCG10300.012.17523913
GTATCAA64450.012.1415091
CGTCTTA3300.011.8030915
CGCGTAA9200.011.77362610
CGAACGA4300.011.70895816
GCGTAAC9250.011.60716611
CGGACCA20400.011.5506739
GCATCGC11050.011.43464414
CGGTCCA11750.011.40182810
ACGGTAT13450.011.3733749
GCGTTAT7750.011.2733891
CGCCAGT11300.011.26466818
CGCAAGA19250.011.2496742
CGACCAT13500.011.19068310
GTATTAT2550.011.1724761
Produced by FastQC (version 0.11.5)