##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062313_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5802551
Sequences flagged as poor quality	0
Sequence length	25
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.34341257836424	32.0	32.0	32.0	32.0	32.0
2	31.032714921419906	32.0	32.0	32.0	32.0	32.0
3	31.051809281814155	32.0	32.0	32.0	32.0	32.0
4	31.055414937326702	32.0	32.0	32.0	32.0	32.0
5	31.009455324046268	32.0	32.0	32.0	32.0	32.0
6	34.6095010625499	36.0	36.0	36.0	32.0	36.0
7	34.57430464635296	36.0	36.0	36.0	32.0	36.0
8	34.55845420402164	36.0	36.0	36.0	32.0	36.0
9	34.6604445182817	36.0	36.0	36.0	32.0	36.0
10	34.456223305921824	36.0	36.0	36.0	32.0	36.0
11	34.591419015533	36.0	36.0	36.0	32.0	36.0
12	34.50811496529716	36.0	36.0	36.0	32.0	36.0
13	34.5642766431523	36.0	36.0	36.0	32.0	36.0
14	34.49686767078825	36.0	36.0	36.0	32.0	36.0
15	34.46071788080794	36.0	36.0	36.0	32.0	36.0
16	34.454412722955816	36.0	36.0	36.0	32.0	36.0
17	34.40074219080539	36.0	36.0	36.0	32.0	36.0
18	34.40411260495599	36.0	36.0	36.0	32.0	36.0
19	34.404636857134044	36.0	36.0	36.0	32.0	36.0
20	34.389979338397886	36.0	36.0	36.0	32.0	36.0
21	34.36201095001147	36.0	36.0	36.0	32.0	36.0
22	34.336805829022445	36.0	36.0	36.0	32.0	36.0
23	34.32011248156199	36.0	36.0	36.0	32.0	36.0
24	34.29938056554781	36.0	36.0	36.0	32.0	36.0
25	33.95151253302211	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	5.0
4	56.0
5	446.0
6	1257.0
7	218.0
8	960.0
9	819.0
10	416.0
11	112.0
12	230.0
13	241.0
14	604.0
15	1022.0
16	1754.0
17	2437.0
18	3351.0
19	4427.0
20	6208.0
21	8630.0
22	12130.0
23	17409.0
24	24572.0
25	35345.0
26	49203.0
27	65896.0
28	90552.0
29	123858.0
30	168745.0
31	237546.0
32	351419.0
33	521938.0
34	1234602.0
35	2836143.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.03445286651669	16.519580082854258	11.130796187421995	26.315170863207065
2	17.689541262647918	18.58020355237779	35.97470140641954	27.755553778554752
3	19.82749231196631	21.454267143057056	27.32357505113292	31.394665493843714
4	13.361657095188523	14.612923908472307	33.47156822866755	38.55385076767162
5	15.881396544789627	35.34855953023951	32.356820610653465	16.413223314317396
6	36.7901924780165	33.3428811277181	15.62266334833389	14.244263045931502
7	32.440719979407866	28.88650656236263	19.11319601103308	19.55957744719643
8	29.345972561522643	32.181017349178084	18.12211019557506	20.350899893724208
9	26.822031573356963	14.267934929082971	18.18610039329524	40.72393310426482
10	17.325002735181613	25.581316690544142	29.435178956806908	27.658501617467333
11	38.50629888415427	20.60204620326489	20.76827454737206	20.12338036520878
12	24.90541533041302	23.024662767083914	27.280334395893703	24.789587506609365
13	29.72473112787383	18.36866930083066	24.63796350524423	27.26863606605128
14	24.176106684058556	18.76926501150401	23.580831308161677	33.47379699627576
15	25.967894044306387	26.355098254923533	21.026821113483898	26.65018658728618
16	27.01998956454042	24.91911138472751	22.410132196291546	25.65076685444052
17	24.920924285575012	25.481835146698366	23.92908507530548	25.668155492421135
18	25.956801982314516	24.089445006800318	24.752154612802883	25.201598398082282
19	26.22173636808546	24.270052940428606	24.151161665395584	25.357049026090355
20	26.57334406488055	23.449735891471075	23.640878639103654	26.336041404544712
21	27.447067803407638	23.423487759335202	23.513754306980434	25.615690130276725
22	27.028323678879847	23.24949272284571	24.02366614831987	25.69851744995458
23	25.24569161117021	23.517627410454658	24.576612197314954	26.660068781060183
24	25.353933834373766	24.159078324797704	24.419752745688918	26.06723509513961
25	25.604946585173423	23.53110520583691	24.30804970595713	26.555898503032537
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	239.0
1	239.0
2	689.5
3	1140.0
4	1140.0
5	1140.0
6	2403.5
7	3667.0
8	3667.0
9	3667.0
10	4310.0
11	4953.0
12	4953.0
13	4953.0
14	6230.5
15	7508.0
16	7508.0
17	7508.0
18	12895.0
19	18282.0
20	18282.0
21	18282.0
22	31028.5
23	43775.0
24	43775.0
25	43775.0
26	70661.0
27	97547.0
28	97547.0
29	97547.0
30	129256.5
31	160966.0
32	160966.0
33	160966.0
34	220372.0
35	279778.0
36	279778.0
37	279778.0
38	348860.0
39	417942.0
40	417942.0
41	417942.0
42	496056.5
43	574171.0
44	574171.0
45	574171.0
46	659894.0
47	745617.0
48	745617.0
49	745617.0
50	789278.0
51	832939.0
52	832939.0
53	832939.0
54	792674.0
55	752409.0
56	752409.0
57	752409.0
58	704434.5
59	656460.0
60	656460.0
61	656460.0
62	587215.5
63	517971.0
64	517971.0
65	517971.0
66	426558.5
67	335146.0
68	335146.0
69	335146.0
70	254777.0
71	174408.0
72	174408.0
73	174408.0
74	132254.5
75	90101.0
76	90101.0
77	90101.0
78	71710.5
79	53320.0
80	53320.0
81	53320.0
82	36742.5
83	20165.0
84	20165.0
85	20165.0
86	14498.5
87	8832.0
88	8832.0
89	8832.0
90	5813.0
91	2794.0
92	2794.0
93	2794.0
94	1624.0
95	454.0
96	454.0
97	454.0
98	1210.5
99	1967.0
100	1967.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0012580673569262898
2	2.5850699114923764E-4
3	4.653125840686277E-4
4	0.010185175451279964
5	0.03408845523287947
6	0.06298953684336424
7	0.10621190576351677
8	0.10876250807618924
9	0.11899938492569906
10	0.1325106836630992
11	0.1398005808135077
12	0.13344130883123645
13	0.12782309022359303
14	0.11625921081951714
15	0.13228664427076986
16	0.11937852851271794
17	0.12332506857759631
18	0.11198522856584975
19	0.09750883706149244
20	0.09156317626505997
21	0.09092552568689184
22	0.10197239110866928
23	0.0815848064066994
24	0.0910116946839416
25	0.08784067559251095
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	5802551.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.21286479259007
#Duplication Level	Percentage of deduplicated	Percentage of total
1	63.52684346803578	19.828547758678347
2	16.10679795742994	10.054786137736533
3	7.128398924537698	6.674932554577189
4	3.8925402367881814	4.859893284423443
5	2.238820747396706	3.4940004641669424
6	1.4442117990310122	2.704679256901095
7	0.9906922686214461	2.1645640682081866
8	0.7140372046332827	1.7829717380078098
9	0.5574559953000116	1.5659818748206191
>10	2.9496701347593843	17.171871128209432
>50	0.2587195885950107	5.577379007591176
>100	0.15959402490240923	9.487768763410733
>500	0.01682846700474817	3.6615608449549932
>1k	0.014337404272524201	8.743285407230172
>5k	9.96421918553303E-4	2.041965423362539
>10k+	5.535677325296128E-5	0.18581228772077124
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCGCAAGACGGACCAGAGCGAAAGC	10754	0.18533227885459344	No Hit
GGGTAGGCACACGCTGAGCCAGTCA	8491	0.14633219078987844	No Hit
GAATAGGACCGCGGTTCTATTTTGT	7808	0.13456150579288317	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	7460	0.12856414359822085	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	7324	0.12622034687846778	No Hit
TCGTAGTTCCGACCATAAACGATGC	7308	0.12594460608790858	No Hit
ATCAGATACCGTCGTAGTTCCGACC	7073	0.12189466322657051	No Hit
GTGTAGCGCGCGTGCAGCCCCGGAC	7067	0.12179126043011083	No Hit
GTATCAACGCAGAGTACTTTTTTTT	6755	0.11641431501420668	No Hit
GAACTACGACGGTATCTGATCGTCT	6652	0.11463923367498192	No Hit
GTGCATGGCCGTTCTTAGTTGGTGG	6427	0.11076162880774335	No Hit
CTCCTGGTGGTGCCCTTCCGTCAAT	6188	0.1066427507487655	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	6004	0.10347173165733486	No Hit
GCCATGCACCACCACCCACGGAATC	5857	0.10093836314407233	No Hit
GAATAACGCCGCCGCATCGCCAGTC	5846	0.10074879135056289	No Hit
GTATCTGATCGTCTTCGAACCTCCG	5826	0.1004041153623639	No Hit
ACCATACTCCCCCCGGAACCCAAAG	5812	0.10016284217062461	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.2063659586964424E-4
2	0.0	0.0	0.0	0.0	1.378703952795934E-4
3	0.0	0.0	0.0	0.0	1.378703952795934E-4
4	0.0	0.0	0.0	0.0	1.378703952795934E-4
5	0.0	0.0	0.0	0.0	1.8957179350944094E-4
6	0.0	0.0	0.0	0.0	2.5850699114923764E-4
7	0.0	0.0	0.0	0.0	2.5850699114923764E-4
8	0.0	0.0	0.0	0.0	2.5850699114923764E-4
9	0.0	0.0	0.0	0.0	2.5850699114923764E-4
10	0.0	0.0	0.0	1.7233799409949176E-5	2.5850699114923764E-4
11	0.0	0.0	0.0	3.446759881989835E-5	2.5850699114923764E-4
12	0.0	0.0	0.0	5.170139822984753E-5	6.031829793482212E-4
13	0.0	0.0	0.0	5.170139822984753E-5	7.755209734477129E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2535	0.0	14.984781	1
AAGACGG	1940	0.0	12.928347	5
CAAGACG	2000	0.0	12.587236	4
TCGCGTA	870	0.0	12.450057	9
CGTTATT	715	0.0	12.221307	2
CGCATCG	1030	0.0	12.175239	13
GTATCAA	6445	0.0	12.141509	1
CGTCTTA	330	0.0	11.80309	15
CGCGTAA	920	0.0	11.773626	10
CGAACGA	430	0.0	11.708958	16
GCGTAAC	925	0.0	11.607166	11
CGGACCA	2040	0.0	11.550673	9
GCATCGC	1105	0.0	11.434644	14
CGGTCCA	1175	0.0	11.401828	10
ACGGTAT	1345	0.0	11.373374	9
GCGTTAT	775	0.0	11.273389	1
CGCCAGT	1130	0.0	11.264668	18
CGCAAGA	1925	0.0	11.249674	2
CGACCAT	1350	0.0	11.190683	10
GTATTAT	255	0.0	11.172476	1
>>END_MODULE