FastQCFastQC Report
Thu 2 Feb 2017
SRR4062313_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062313_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5802551
Sequences flagged as poor quality0
Sequence length50
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT101550.1750092330080339No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC81300.1401107892028868No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT80700.13907676123828985No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT77220.13307939904362753No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA74980.12921902797579893No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG73110.12599630748613844No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA72000.12408335575163407No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA67970.11713813458942456No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA65700.11322606212336608No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG60520.1042989540290124No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC59930.10328215986382541No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGAT58810.10135197432991111No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATCTGTCAAT58720.10119687013522156No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA65650.027.4880941
GGTATCA28800.026.5156651
CGCAATA16450.019.526236
ATACGAA17400.018.71395940
TAGCGGC17400.018.08048430
CGAATGC18050.017.91846743
TACGAAT17950.017.89540941
TCTAGCG18000.017.84462228
TCAACGC100100.017.7573494
CTAGCGG18100.017.50278729
ATCAACG103250.017.258363
GCGCAAT18700.017.17634635
CAACGCA103600.017.1362025
AACGCAG104650.016.9852896
CAATACG19150.016.88891638
GTATAGG6500.016.590071
TACCGTA4150.016.4329077
TATCAAC111900.016.2202822
AATACGA19900.016.14183839
GATTAAT20100.015.87176338
Produced by FastQC (version 0.11.5)