##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062313_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5802551
Sequences flagged as poor quality	0
Sequence length	50
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.25548659546465	32.0	32.0	32.0	32.0	32.0
2	31.376185060674175	32.0	32.0	32.0	32.0	32.0
3	31.525030456432006	32.0	32.0	32.0	32.0	32.0
4	31.613121883805935	32.0	32.0	32.0	32.0	32.0
5	31.565450781906097	32.0	32.0	32.0	32.0	32.0
6	35.20916627876257	36.0	36.0	36.0	36.0	36.0
7	35.21467592443393	36.0	36.0	36.0	36.0	36.0
8	35.153286718203766	36.0	36.0	36.0	36.0	36.0
9	35.26266671331282	36.0	36.0	36.0	36.0	36.0
10	35.11908193482487	36.0	36.0	36.0	36.0	36.0
11	35.25364723205362	36.0	36.0	36.0	36.0	36.0
12	35.163059833511156	36.0	36.0	36.0	36.0	36.0
13	35.214609401968204	36.0	36.0	36.0	36.0	36.0
14	35.16723334271426	36.0	36.0	36.0	36.0	36.0
15	35.13804359496366	36.0	36.0	36.0	36.0	36.0
16	35.152723345301055	36.0	36.0	36.0	36.0	36.0
17	35.12984202982447	36.0	36.0	36.0	36.0	36.0
18	35.129992480893314	36.0	36.0	36.0	36.0	36.0
19	35.12009011209035	36.0	36.0	36.0	36.0	36.0
20	35.1229102510258	36.0	36.0	36.0	36.0	36.0
21	35.11041126566574	36.0	36.0	36.0	36.0	36.0
22	35.10058351921422	36.0	36.0	36.0	36.0	36.0
23	35.067492383953194	36.0	36.0	36.0	36.0	36.0
24	35.04553066401312	36.0	36.0	36.0	36.0	36.0
25	35.03375687693223	36.0	36.0	36.0	36.0	36.0
26	34.98479892723046	36.0	36.0	36.0	36.0	36.0
27	34.976044674144184	36.0	36.0	36.0	36.0	36.0
28	34.95591059863153	36.0	36.0	36.0	32.0	36.0
29	34.93366365931122	36.0	36.0	36.0	32.0	36.0
30	34.91815754829212	36.0	36.0	36.0	32.0	36.0
31	34.927042433577924	36.0	36.0	36.0	32.0	36.0
32	34.898628551476754	36.0	36.0	36.0	32.0	36.0
33	34.87798125341768	36.0	36.0	36.0	32.0	36.0
34	34.868880945639255	36.0	36.0	36.0	32.0	36.0
35	34.852074199778684	36.0	36.0	36.0	32.0	36.0
36	34.83301792608113	36.0	36.0	36.0	32.0	36.0
37	34.82800599253673	36.0	36.0	36.0	32.0	36.0
38	34.807038145808626	36.0	36.0	36.0	32.0	36.0
39	34.79683246213605	36.0	36.0	36.0	32.0	36.0
40	34.78263112207028	36.0	36.0	36.0	32.0	36.0
41	34.75752406139989	36.0	36.0	36.0	32.0	36.0
42	34.72116746582667	36.0	36.0	36.0	32.0	36.0
43	34.72647530370694	36.0	36.0	36.0	32.0	36.0
44	34.67255040067722	36.0	36.0	36.0	32.0	36.0
45	34.6628043424349	36.0	36.0	36.0	32.0	36.0
46	34.65386172392108	36.0	36.0	36.0	32.0	36.0
47	34.63910063005047	36.0	36.0	36.0	32.0	36.0
48	34.610199893115976	36.0	36.0	36.0	32.0	36.0
49	34.60342494189194	36.0	36.0	36.0	32.0	36.0
50	34.16320097832833	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	4.0
19	9.0
20	32.0
21	98.0
22	298.0
23	921.0
24	2365.0
25	6012.0
26	12996.0
27	25512.0
28	44647.0
29	72583.0
30	110453.0
31	168627.0
32	260943.0
33	455082.0
34	1048418.0
35	3593551.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.42659048363777	17.281507773023808	11.59700087936647	26.694900863971956
2	17.034444949418962	19.04208919504477	35.71760584171065	28.205860013825617
3	19.170434847947465	21.942292798284335	27.610040716693774	31.277231637074422
4	13.04438565802966	15.048073691738978	33.7328588293078	38.17468182092356
5	15.526343499609052	35.69778188937934	32.19306473997385	16.582809871037757
6	36.924426729600015	33.52657051453837	15.473479635904338	14.075523119957273
7	32.45644889635611	28.908474910431636	19.10420089371037	19.53087529950189
8	29.356329655697987	32.24721333771991	18.050543631585487	20.34591337499662
9	27.019460923307697	13.92246272372272	18.06221091378602	40.99586543918356
10	17.272015673114637	25.57123181057701	29.516515847865456	27.640236668442896
11	38.76708709669247	20.428135831981486	20.751665948304463	20.05311112302158
12	24.937979864373442	22.892896589793008	27.28953179386101	24.879591751972537
13	29.83884157157774	18.264742524451748	24.65446663028037	27.241949273690143
14	24.313961221538598	18.558182427004947	23.57731969955973	33.55053665189673
15	26.09605671712321	26.222259830202265	21.01343012754218	26.66825332513234
16	27.136930404512388	24.857816013292165	22.356246435813976	25.649007146381475
17	25.12577657654366	25.344180516465947	23.860850167452213	25.669192739538175
18	26.178663641787587	23.955760109441616	24.65314363729301	25.21243261147779
19	26.363439114968575	24.199442624459483	24.109240918347812	25.327877342224138
20	26.788264283520657	23.232554235862104	23.63580964566122	26.34337183495602
21	27.68049776727512	23.2814153636909	23.420405955932143	25.617680913101843
22	27.110627722186326	23.106854209467524	24.057453351120913	25.725064717225234
23	25.343810108281723	23.33355564942235	24.583023771104735	26.739610471191195
24	25.543779199733628	24.025414593348067	24.365527024332472	26.065279182585833
25	25.816904760550674	23.32117142432206	24.38386066247161	26.478063152655658
26	25.50784356810182	24.575025527679763	24.533250610725503	25.383880293492915
27	26.17014595717312	23.601332799312853	24.152368865686725	26.076152377827306
28	25.767002271450384	23.514767874315375	24.65216477149347	26.06606508274077
29	25.33222209181909	23.909512403147666	24.880297677612084	25.877967827421163
30	25.36087679977389	24.165022968686582	24.88259730993638	25.591502921603148
31	26.091228278191497	23.831296776004773	23.80124087444061	26.27623407136312
32	26.120784531076097	23.671218844022004	23.674355424163867	26.53364120073803
33	25.35466401403249	23.66012848869132	24.27186122476835	26.713346272507838
34	26.322507995694266	23.670632889490008	24.342929545171753	25.663929569643972
35	26.730230838762715	23.330751128601552	24.636180565907832	25.302837466727908
36	25.206508523415156	24.20627500519169	24.090549922490904	26.496666548902247
37	26.531068422136567	23.87537301150293	24.141519896388328	25.452038669972175
38	25.58698751635272	23.462852803878846	24.455898793478937	26.494260886289496
39	26.564956642031923	23.60465102251457	23.64637435288769	26.184017982565816
40	26.38678468622626	23.83435119200364	24.21566689490001	25.563197226870088
41	25.258880239345523	24.472872852861443	24.691951214047762	25.576295693745273
42	26.971294259093103	24.128071608033704	24.000655607286408	24.89997852558678
43	26.2800384746169	23.20615373267035	24.234131245597627	26.279676547115123
44	25.505195518748746	24.095428922421906	24.03279867252824	26.366576886301107
45	25.763544013390284	24.261187064147073	24.06018458517969	25.915084337282952
46	25.579411005892215	24.02019439883593	24.244842781462363	26.155551813809492
47	25.795662586982036	23.86213093652364	24.630197616851106	25.71200885964321
48	26.634185434287115	24.5474184917253	23.313326985303306	25.50506908868428
49	25.686761365193338	24.510788141962653	23.65323801092076	26.14921248192325
50	26.040545196065878	24.347764044337726	23.77171556285667	25.83997519673973
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	34.0
1	53.5
2	73.0
3	185.5
4	298.0
5	371.0
6	444.0
7	459.0
8	474.0
9	608.0
10	742.0
11	1058.0
12	1374.0
13	2358.0
14	3342.0
15	4344.0
16	5346.0
17	5320.0
18	5294.0
19	5035.0
20	4776.0
21	5456.0
22	6136.0
23	7318.0
24	8500.0
25	11036.5
26	13573.0
27	17605.5
28	21638.0
29	27141.5
30	32645.0
31	40279.0
32	47913.0
33	58669.5
34	69426.0
35	85054.0
36	100682.0
37	130388.5
38	160095.0
39	187537.0
40	214979.0
41	241182.5
42	267386.0
43	283777.5
44	300169.0
45	334918.5
46	369668.0
47	402049.0
48	434430.0
49	458549.0
50	482668.0
51	472147.0
52	461626.0
53	464560.5
54	467495.0
55	475378.0
56	483261.0
57	470315.0
58	457369.0
59	422283.0
60	387197.0
61	343431.5
62	299666.0
63	259586.5
64	219507.0
65	186448.0
66	153389.0
67	132055.0
68	110721.0
69	98357.0
70	85993.0
71	66025.0
72	46057.0
73	39778.5
74	33500.0
75	24375.0
76	15250.0
77	13079.0
78	10908.0
79	9297.5
80	7687.0
81	6168.5
82	4650.0
83	3858.5
84	3067.0
85	2496.5
86	1926.0
87	1337.0
88	748.0
89	477.5
90	207.0
91	146.0
92	85.0
93	69.0
94	53.0
95	41.5
96	30.0
97	33.0
98	36.0
99	27.0
100	18.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.030745098147349333
2	0.004790996235965871
3	2.92974589969136E-4
4	1.7233799409949176E-5
5	0.0
6	1.7233799409949177E-4
7	0.0
8	0.0
9	0.0
10	5.170139822984752E-5
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	5.85949179938272E-4
17	0.0
18	8.616899704974589E-5
19	0.0
20	4.308449852487294E-4
21	0.0
22	0.0
23	3.446759881989835E-5
24	9.995603657770523E-4
25	1.378703952795934E-4
26	4.480787846586786E-4
27	4.653125840686277E-4
28	0.001568275746305375
29	5.514815811183736E-4
30	0.0014821067492556293
31	5.170139822984753E-4
32	9.133913687273063E-4
33	2.92974589969136E-4
34	9.133913687273063E-4
35	2.757407905591868E-4
36	1.0340279645969504E-4
37	0.0019991207315541047
38	0.0
39	7.238195752178654E-4
40	1.7233799409949177E-4
41	0.0010512617640068998
42	0.005118438424754905
43	0.004894399032425566
44	0.004187813256617649
45	0.002326562920343139
46	0.0025850699114923765
47	0.0010340279645969506
48	0.003894838666648514
49	0.003584630277269429
50	0.0015165743480755275
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	5802551.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.22720218812484
#Duplication Level	Percentage of deduplicated	Percentage of total
1	62.93013379317976	20.28062145478552
2	16.557658405561142	10.672140103958474
3	7.300126947990606	7.057880014556159
4	3.8686802236352937	4.987069590731785
5	2.2719274088312353	3.6608931980573396
6	1.4935691701714584	2.888013337743926
7	1.012278914240638	2.283604206800597
8	0.7191395713268416	1.854068509330524
9	0.5204611939641428	1.5095707316059759
>10	2.9053964837069706	17.256268360311218
>50	0.2439036055449767	5.440217585935889
>100	0.1481965157992864	9.022025297290824
>500	0.015121865934809216	3.4058519947782564
>1k	0.01249429890517307	7.686103861512226
>5k	8.579776072206977E-4	1.8201792763699998
>10k+	5.3623600451293604E-5	0.17549247623129471
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	10155	0.1750092330080339	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC	8130	0.1401107892028868	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	8070	0.13907676123828985	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT	7722	0.13307939904362753	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	7498	0.12921902797579893	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG	7311	0.12599630748613844	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	7200	0.12408335575163407	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA	6797	0.11713813458942456	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA	6570	0.11322606212336608	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG	6052	0.1042989540290124	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	5993	0.10328215986382541	No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGAT	5881	0.10135197432991111	No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATCTGTCAAT	5872	0.10119687013522156	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.378703952795934E-4
2	0.0	0.0	0.0	0.0	1.378703952795934E-4
3	0.0	0.0	0.0	0.0	1.378703952795934E-4
4	0.0	0.0	0.0	0.0	1.5510419468954257E-4
5	0.0	0.0	0.0	0.0	1.7233799409949177E-4
6	0.0	0.0	0.0	0.0	2.068055929193901E-4
7	0.0	0.0	0.0	1.7233799409949176E-5	2.068055929193901E-4
8	0.0	0.0	0.0	1.7233799409949176E-5	2.068055929193901E-4
9	0.0	0.0	0.0	1.7233799409949176E-5	2.068055929193901E-4
10	0.0	0.0	0.0	1.7233799409949176E-5	2.068055929193901E-4
11	0.0	0.0	0.0	1.7233799409949176E-5	2.068055929193901E-4
12	0.0	0.0	0.0	3.446759881989835E-5	4.653125840686277E-4
13	0.0	0.0	0.0	3.446759881989835E-5	5.85949179938272E-4
14	0.0	0.0	0.0	3.446759881989835E-5	5.85949179938272E-4
15	0.0	0.0	0.0	1.0340279645969505E-4	7.755209734477129E-4
16	0.0	0.0	0.0	1.5510419468954257E-4	8.616899704974588E-4
17	0.0	0.0	0.0	1.7233799409949177E-4	8.616899704974588E-4
18	0.0	0.0	0.0	2.5850699114923764E-4	9.133913687273063E-4
19	0.0	0.0	0.0	2.92974589969136E-4	9.133913687273063E-4
20	0.0	0.0	0.0	3.1020838937908515E-4	0.0010340279645969506
21	0.0	0.0	0.0	3.9637738642883105E-4	0.0011201969616466965
22	0.0	0.0	0.0	4.997801828885261E-4	0.0011546645604665947
23	0.0	0.0	0.0	6.031829793482212E-4	0.0012063659586964424
24	0.0	0.0	0.0	7.927547728576621E-4	0.0012063659586964424
25	0.0	0.0	0.0	0.001085729362826798	0.0012063659586964424
26	0.0	0.0	0.0	0.0013442363539760357	0.0012580673569262898
27	0.0	0.0	0.0	0.0016199771445352224	0.0012925349557461883
28	0.0	0.0	0.0	0.002343796719753088	0.0013442363539760357
29	0.0	0.0	0.0	0.004239514654847497	0.0013442363539760357
30	0.0	0.0	0.0	0.007979249126806469	0.001361470153385985
31	0.0	0.0	0.0	0.014562560501407053	0.0013787039527959342
32	0.0	0.0	0.0	0.02173182105594591	0.0013959377522058832
33	0.0	0.0	0.0	0.02864257461933553	0.0014131715516158324
34	0.0	0.0	0.0	0.03675969414142159	0.0015165743480755275
35	0.0	0.0	0.0	0.04660019360450257	0.0015338081474854767
36	0.0	0.0	0.0	0.06173146948643795	0.0015338081474854767
37	0.0	0.0	0.0	0.08272223716775605	0.0015338081474854767
38	0.0	0.0	0.0	0.1128813861351671	0.0016372109439451716
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	6565	0.0	27.488094	1
GGTATCA	2880	0.0	26.515665	1
CGCAATA	1645	0.0	19.5262	36
ATACGAA	1740	0.0	18.713959	40
TAGCGGC	1740	0.0	18.080484	30
CGAATGC	1805	0.0	17.918467	43
TACGAAT	1795	0.0	17.895409	41
TCTAGCG	1800	0.0	17.844622	28
TCAACGC	10010	0.0	17.757349	4
CTAGCGG	1810	0.0	17.502787	29
ATCAACG	10325	0.0	17.25836	3
GCGCAAT	1870	0.0	17.176346	35
CAACGCA	10360	0.0	17.136202	5
AACGCAG	10465	0.0	16.985289	6
CAATACG	1915	0.0	16.888916	38
GTATAGG	650	0.0	16.59007	1
TACCGTA	415	0.0	16.432907	7
TATCAAC	11190	0.0	16.220282	2
AATACGA	1990	0.0	16.141838	39
GATTAAT	2010	0.0	15.871763	38
>>END_MODULE