FastQCFastQC Report
Thu 2 Feb 2017
SRR4062312_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062312_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1531080
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT21260.13885623220210572No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA4150.012.8148881
GACCGTC701.0897043E-412.2156947
GTCTTAT1201.0062649E-811.8709341
GTATCAA9900.011.7989881
CCGCGCG658.0133346E-411.6940389
TTACACG1001.9289237E-611.4001964
GTAGCGC752.075962E-411.39870553
GTATAAC1053.4797395E-610.8534241
CCATATA803.7779834E-410.6852382
CGTGTAC1002.4013128E-510.44881253
TCTATAC1303.8456892E-710.2296073
CTAAGGT1054.1033152E-59.9525524
CTTATAC1054.109218E-59.951253
GACAGCG1151.0246982E-59.91418657
GTGTAAG2550.09.6829581
ACCGTCC1002.7515832E-49.5010958
GCACCGC1002.7524875E-49.5007846
TAGACAG1606.6247594E-89.5004745
CCTAGAC1804.1854946E-99.4989213
GTTGGAC900.00111464899.4989213
Produced by FastQC (version 0.11.5)