##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR4062312_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1531080 Sequences flagged as poor quality 0 Sequence length 25 %GC 50 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.264854873683934 32.0 32.0 32.0 32.0 32.0 2 30.900427149463123 32.0 32.0 32.0 32.0 32.0 3 30.917972281004257 32.0 32.0 32.0 32.0 32.0 4 30.924797528541944 32.0 32.0 32.0 32.0 32.0 5 30.874199258040075 32.0 32.0 32.0 32.0 32.0 6 34.46166692792016 36.0 36.0 36.0 32.0 36.0 7 34.43019698513468 36.0 36.0 36.0 32.0 36.0 8 34.4021390129843 36.0 36.0 36.0 32.0 36.0 9 34.515999817122555 36.0 36.0 36.0 32.0 36.0 10 34.27774969302715 36.0 36.0 36.0 32.0 36.0 11 34.4757837604828 36.0 36.0 36.0 32.0 36.0 12 34.366956004911565 36.0 36.0 36.0 32.0 36.0 13 34.40543015387831 36.0 36.0 36.0 32.0 36.0 14 34.347002116153305 36.0 36.0 36.0 32.0 36.0 15 34.30726676594299 36.0 36.0 36.0 32.0 36.0 16 34.31487708023095 36.0 36.0 36.0 32.0 36.0 17 34.26493651540089 36.0 36.0 36.0 32.0 36.0 18 34.25937312224051 36.0 36.0 36.0 32.0 36.0 19 34.258216422394646 36.0 36.0 36.0 32.0 36.0 20 34.24190701988139 36.0 36.0 36.0 32.0 36.0 21 34.23141638581916 36.0 36.0 36.0 32.0 36.0 22 34.19514068500666 36.0 36.0 36.0 32.0 36.0 23 34.17336585939337 36.0 36.0 36.0 32.0 36.0 24 34.146085116388434 36.0 36.0 36.0 32.0 36.0 25 33.79457768372652 36.0 36.0 36.0 32.0 36.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 3.0 4 23.0 5 101.0 6 331.0 7 69.0 8 273.0 9 193.0 10 132.0 11 31.0 12 56.0 13 61.0 14 191.0 15 351.0 16 537.0 17 733.0 18 993.0 19 1331.0 20 1828.0 21 2503.0 22 3479.0 23 5139.0 24 7308.0 25 10280.0 26 14668.0 27 19759.0 28 27221.0 29 37313.0 30 50688.0 31 69770.0 32 101195.0 33 145722.0 34 322965.0 35 705833.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.77926716083443 17.26667620255 11.723780729547327 26.230275907068247 2 16.54245143457653 19.047482200461243 37.67944044690052 26.73062591806171 3 19.29601005308042 22.263144866378024 28.316350690006292 30.12449439053527 4 13.305816326264 15.221642764015645 34.73936138247812 36.73317952724223 5 15.02917855761491 35.871985448615426 34.00628260210535 15.092553391664316 6 35.504779979766454 34.24240523039842 16.22886130085509 14.023953488980034 7 30.562693122959256 30.11966267965421 20.257266261557955 19.060377935828583 8 27.658940111569958 34.17974113468685 18.853844926653085 19.307473827090107 9 26.412095040306337 15.159538294945099 18.56112125381223 39.86724541093633 10 15.845297016752644 27.72891008402482 32.152769784549065 24.273023114673475 11 36.58458419176559 21.55328820432323 21.98718074495569 19.874946858955493 12 23.785170254705175 23.619905536858546 29.2925989920618 23.302325216374484 13 29.528800810389622 20.041055729981736 24.787903796968365 25.642239662660277 14 22.697541572642844 19.421033393469347 25.59891349764698 32.28251153624083 15 24.797279400945015 27.36641433546425 22.178310416428232 25.6579958471625 16 25.166484444886194 26.417929576949035 23.68381818276932 24.731767795395456 17 23.785399883730587 26.433635210929584 25.470063895862367 24.310901009477462 18 24.190072420746493 26.20662012977672 25.55825960052204 24.045047848954752 19 24.99475352004482 25.735144616523176 25.404665411869924 23.86543645156208 20 25.10585609585329 25.403109557545378 25.367480981204455 24.123553365396873 21 24.965385143395068 25.191640136236753 25.40495133000804 24.43802339036014 22 25.03628627319706 25.02059491181455 25.248511935895557 24.69460687909283 23 24.49283613680204 25.2448146233146 25.2869107764191 24.975438463464265 24 24.38342265636543 25.31480975436275 25.43843248211687 24.863335107154953 25 24.460737816959735 25.20982474673619 25.44705630866644 24.88238112763764 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 175.0 1 175.0 2 319.5 3 464.0 4 464.0 5 464.0 6 920.5 7 1377.0 8 1377.0 9 1377.0 10 2024.5 11 2672.0 12 2672.0 13 2672.0 14 3969.5 15 5267.0 16 5267.0 17 5267.0 18 8574.5 19 11882.0 20 11882.0 21 11882.0 22 16865.0 23 21848.0 24 21848.0 25 21848.0 26 31169.0 27 40490.0 28 40490.0 29 40490.0 30 52247.0 31 64004.0 32 64004.0 33 64004.0 34 78179.5 35 92355.0 36 92355.0 37 92355.0 38 110181.0 39 128007.0 40 128007.0 41 128007.0 42 146300.0 43 164593.0 44 164593.0 45 164593.0 46 180645.5 47 196698.0 48 196698.0 49 196698.0 50 204142.5 51 211587.0 52 211587.0 53 211587.0 54 204527.0 55 197467.0 56 197467.0 57 197467.0 58 177918.0 59 158369.0 60 158369.0 61 158369.0 62 134363.0 63 110357.0 64 110357.0 65 110357.0 66 88642.0 67 66927.0 68 66927.0 69 66927.0 70 50034.0 71 33141.0 72 33141.0 73 33141.0 74 23437.0 75 13733.0 76 13733.0 77 13733.0 78 9813.0 79 5893.0 80 5893.0 81 5893.0 82 4026.5 83 2160.0 84 2160.0 85 2160.0 86 1471.0 87 782.0 88 782.0 89 782.0 90 484.5 91 187.0 92 187.0 93 187.0 94 134.5 95 82.0 96 82.0 97 82.0 98 322.5 99 563.0 100 563.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0013715808448938004 2 6.531337356637144E-5 3 4.5719361496460013E-4 4 0.009666379287822975 5 0.032917940277451214 6 0.06191707814092014 7 0.10541578493612352 8 0.10685267915458369 9 0.11704156543093763 10 0.13114925412127387 11 0.13911748569637117 12 0.1318023878569376 13 0.12664263134519424 14 0.11553935783891109 15 0.13095331400057475 16 0.11808657940799959 17 0.12233194868981373 18 0.10998772108576953 19 0.09685973299892886 20 0.09202654335501737 21 0.09078558925725631 22 0.10293387674060141 23 0.08157640358439794 24 0.09313687070564569 25 0.08823836768816783 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1531080.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.04586526027336 #Duplication Level Percentage of deduplicated Percentage of total 1 66.04102565499576 35.03203348545163 2 17.190954345483288 18.23818095812035 3 7.145195462239015 11.370692272447421 4 3.5825618913519985 7.6016038150099305 5 1.981147417967416 5.254583949711902 6 1.1788837729929782 3.752094586782493 7 0.7522371680449526 2.793215002191752 8 0.5016712644322049 2.1289269038417364 9 0.3400464035865833 1.6234250136204993 >10 1.254026786154806 10.435381520275769 >50 0.025947308226576492 0.8996179753945347 >100 0.005808251928207949 0.5487325883013523 >500 2.4713629811383853E-4 0.09373334521028642 >1k 2.4713629811383853E-4 0.22777858364038134 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 2126 0.13885623220210572 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.306267471327429E-4 2 0.0 0.0 0.0 0.0 1.306267471327429E-4 3 0.0 0.0 0.0 0.0 1.306267471327429E-4 4 0.0 0.0 0.0 0.0 1.306267471327429E-4 5 0.0 0.0 0.0 0.0 1.9594012069911436E-4 6 0.0 0.0 0.0 0.0 2.612534942654858E-4 7 0.0 0.0 0.0 0.0 2.612534942654858E-4 8 0.0 0.0 0.0 0.0 3.2656686783185724E-4 9 0.0 0.0 0.0 0.0 3.2656686783185724E-4 10 0.0 0.0 0.0 0.0 3.2656686783185724E-4 11 0.0 0.0 0.0 0.0 3.918802413982287E-4 12 0.0 0.0 0.0 0.0 7.184471092300859E-4 13 0.0 0.0 0.0 0.0 9.143872299292003E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 415 0.0 12.814888 1 GACCGTC 70 1.0897043E-4 12.215694 7 GTCTTAT 120 1.0062649E-8 11.870934 1 GTATCAA 990 0.0 11.798988 1 CCGCGCG 65 8.0133346E-4 11.694038 9 TTACACG 100 1.9289237E-6 11.400196 4 GTAGCGC 75 2.075962E-4 11.3987055 3 GTATAAC 105 3.4797395E-6 10.853424 1 CCATATA 80 3.7779834E-4 10.685238 2 CGTGTAC 100 2.4013128E-5 10.4488125 3 TCTATAC 130 3.8456892E-7 10.229607 3 CTAAGGT 105 4.1033152E-5 9.952552 4 CTTATAC 105 4.109218E-5 9.95125 3 GACAGCG 115 1.0246982E-5 9.9141865 7 GTGTAAG 255 0.0 9.682958 1 ACCGTCC 100 2.7515832E-4 9.501095 8 GCACCGC 100 2.7524875E-4 9.500784 6 TAGACAG 160 6.6247594E-8 9.500474 5 CCTAGAC 180 4.1854946E-9 9.498921 3 GTTGGAC 90 0.0011146489 9.498921 3 >>END_MODULE