FastQCFastQC Report
Thu 2 Feb 2017
SRR4062312_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062312_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1531080
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAACGC407.0354063E-427.49838811
GTATCAA10600.025.1232221
GGTATCA5100.020.714141
ATCAACG15400.016.8561533
TCAACGC15400.016.8561534
AACGCAG15750.016.6212486
CAACGCA15650.016.5868875
TATCAAC16450.016.0482162
CGCAATA850.00297964415.53002436
GTCCTAG3300.015.3394471
ATAAGAC1707.599192E-714.234463
GCACCGT1554.5710276E-614.1927176
CCCTTAA2103.9963197E-813.6244771
GCATAAG1302.3232834E-413.54385951
TAAGACC2451.5352271E-913.4685974
TGCACCG2155.472066E-813.3015465
TATAAGA2653.4924597E-1013.2826742
GCTCTAT2502.062734E-913.2052631
TCGCGGT1000.00876528813.19922617
GTATTAT3400.012.9463371