##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062312_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1531080
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.21839681793244	32.0	32.0	32.0	32.0	32.0
2	31.335911905321733	32.0	32.0	32.0	32.0	32.0
3	31.49922473025577	32.0	32.0	32.0	32.0	32.0
4	31.592176110980486	32.0	32.0	32.0	32.0	32.0
5	31.553799932074092	32.0	32.0	32.0	32.0	32.0
6	35.172930872325416	36.0	36.0	36.0	36.0	36.0
7	35.18905282545654	36.0	36.0	36.0	36.0	36.0
8	35.125849073856365	36.0	36.0	36.0	36.0	36.0
9	35.238101862737416	36.0	36.0	36.0	36.0	36.0
10	35.0881730543146	36.0	36.0	36.0	36.0	36.0
11	35.22691760064791	36.0	36.0	36.0	36.0	36.0
12	35.15874937952295	36.0	36.0	36.0	36.0	36.0
13	35.18752710505003	36.0	36.0	36.0	36.0	36.0
14	35.15231013402304	36.0	36.0	36.0	36.0	36.0
15	35.116835828304204	36.0	36.0	36.0	36.0	36.0
16	35.143307338610654	36.0	36.0	36.0	36.0	36.0
17	35.12612143062413	36.0	36.0	36.0	36.0	36.0
18	35.118797842046135	36.0	36.0	36.0	36.0	36.0
19	35.11359628497531	36.0	36.0	36.0	36.0	36.0
20	35.11849740052773	36.0	36.0	36.0	36.0	36.0
21	35.1176104449147	36.0	36.0	36.0	36.0	36.0
22	35.089737309611515	36.0	36.0	36.0	36.0	36.0
23	35.05472934137994	36.0	36.0	36.0	36.0	36.0
24	35.04656712908535	36.0	36.0	36.0	36.0	36.0
25	35.03575254069023	36.0	36.0	36.0	36.0	36.0
26	34.99719675000653	36.0	36.0	36.0	36.0	36.0
27	34.98109439088748	36.0	36.0	36.0	36.0	36.0
28	34.95624265224547	36.0	36.0	36.0	36.0	36.0
29	34.94288280168247	36.0	36.0	36.0	36.0	36.0
30	34.919768398777336	36.0	36.0	36.0	32.0	36.0
31	34.92543629333542	36.0	36.0	36.0	32.0	36.0
32	34.89504010241137	36.0	36.0	36.0	32.0	36.0
33	34.87298704182668	36.0	36.0	36.0	32.0	36.0
34	34.86612848446848	36.0	36.0	36.0	32.0	36.0
35	34.858399299840634	36.0	36.0	36.0	32.0	36.0
36	34.83030344593359	36.0	36.0	36.0	32.0	36.0
37	34.82562178331635	36.0	36.0	36.0	32.0	36.0
38	34.8169272670272	36.0	36.0	36.0	32.0	36.0
39	34.79742730621522	36.0	36.0	36.0	32.0	36.0
40	34.78523133996917	36.0	36.0	36.0	32.0	36.0
41	34.77039279462863	36.0	36.0	36.0	32.0	36.0
42	34.74537973195392	36.0	36.0	36.0	32.0	36.0
43	34.729111476866	36.0	36.0	36.0	32.0	36.0
44	34.705436685215666	36.0	36.0	36.0	32.0	36.0
45	34.68906654126499	36.0	36.0	36.0	32.0	36.0
46	34.67841784883873	36.0	36.0	36.0	32.0	36.0
47	34.65109661154218	36.0	36.0	36.0	32.0	36.0
48	34.63222365911644	36.0	36.0	36.0	32.0	36.0
49	34.61638973796275	36.0	36.0	36.0	32.0	36.0
50	34.17332536510176	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	4.0
20	10.0
21	18.0
22	77.0
23	222.0
24	675.0
25	1544.0
26	3306.0
27	6704.0
28	11991.0
29	19611.0
30	30373.0
31	45530.0
32	70178.0
33	120090.0
34	269648.0
35	951099.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.256338138591346	17.78219865870908	12.161778108799403	26.799685093900166
2	15.973452801387843	19.57839568545972	37.25112001301103	27.19703150014141
3	18.647849349997912	22.788673556828158	28.66638538226811	29.89709171090582
4	13.13001280142122	15.701335005355697	34.8518039553779	36.31684823784518
5	14.818895693821155	36.20505538904263	33.693950475449014	15.282098441687204
6	35.59492070292957	34.45892371257376	16.07814885982295	13.868006724673727
7	30.670049899417407	30.045262167881496	20.2505421010006	19.0341458317005
8	27.702928651670717	34.380959845337934	18.761462497060897	19.154649005930455
9	26.502011651905843	14.882762494448363	18.47721869530005	40.138007158345744
10	15.759811061226142	27.75580342738907	32.19561642189359	24.288769089491193
11	36.87534289521122	21.470138725605455	21.798142487655774	19.85637589152755
12	23.749314209577555	23.53358413668783	29.352744467957258	23.364357185777358
13	29.718434046555377	19.94938213548606	24.712751783055097	25.61943203490347
14	22.855566005695326	19.16085377641926	25.587950988844476	32.39562922904094
15	24.860947827677197	27.203607910755807	22.17258405831178	25.762860203255215
16	25.31122066426834	26.232081135521085	23.71847625317752	24.73822194703305
17	23.94433994304674	26.289416620947303	25.426235075894137	24.340008360111817
18	24.39325469162597	26.05985713343335	25.460933106652238	24.085955068288442
19	25.147412284139296	25.654440003135043	25.33649450061394	23.86165321211171
20	25.35695508058064	25.204186600917655	25.37935764087324	24.059500677628463
21	25.224563853334804	25.01993692030261	25.307446578524033	24.44805264783855
22	25.133500535569663	24.982430702510648	25.28319878778379	24.600869974135904
23	24.765149283609794	25.056185866307356	25.255261158960447	24.923403691122402
24	24.507009504501447	25.183728439830077	25.420034694826604	24.889227360841872
25	24.74726891058172	24.900298744541413	25.53900072759383	24.813431617283033
26	24.697190278477176	25.090116303752197	25.45685161490898	24.755841802861646
27	24.66456399360707	25.434680520186248	25.121500234803573	24.779255251403107
28	24.825332630549042	25.21499908886237	25.19697227986229	24.762696000726297
29	24.649477391969413	25.585750105645005	25.251278353518554	24.513494148867036
30	24.85999950361382	25.364615241929478	25.15273672069003	24.622648533766668
31	24.78310933980201	25.219470553619	25.153830227337597	24.843589879241392
32	24.700797872340424	25.421077519541864	25.20051310454374	24.677611503573964
33	24.616479870418267	25.33734357447031	25.25328526269039	24.792891292421036
34	24.828910053518268	25.15632898908342	25.298256247607874	24.71650470979043
35	24.685041967190415	25.23047501856536	25.45587191238586	24.628611101858368
36	24.90033153155036	25.300311676232923	25.292147483207888	24.50720930900883
37	25.049818524065397	25.055566187281077	25.20160908444297	24.693006204210555
38	24.809546202680462	25.30514408130209	25.27588368994435	24.6094260260731
39	24.826935323220614	25.40345835442418	25.169113204774423	24.600493117580786
40	24.99237465865576	25.180803864464213	25.20144290399124	24.625378572888792
41	24.67999503615119	25.218833887412075	25.360826088944332	24.740344987492406
42	24.730179175135987	25.22396891998516	25.310186177022313	24.735665727856535
43	24.80201120690049	25.08561280348212	25.253605273866263	24.85877071575112
44	24.760729063588947	25.207821006463078	25.390511461425664	24.64093846852231
45	24.69786857160399	25.296677615658385	25.25905610503717	24.746397707700456
46	24.77379947003794	25.099392378970553	25.29429092265258	24.832517228338926
47	24.701939694304492	25.316805415311944	25.271346891642814	24.709907998740746
48	24.674335608063622	25.389804470733313	25.17243361305897	24.763426308144094
49	24.682333884167967	25.252480388762972	25.269332009588442	24.795853717480618
50	24.68729242362986	25.443564373249174	25.30777636191326	24.56136684120771
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	82.0
1	65.5
2	49.0
3	126.0
4	203.0
5	212.0
6	221.0
7	173.0
8	125.0
9	181.0
10	237.0
11	432.5
12	628.0
13	1078.5
14	1529.0
15	1897.5
16	2266.0
17	2368.0
18	2470.0
19	2768.5
20	3067.0
21	3896.5
22	4726.0
23	6013.0
24	7300.0
25	8882.5
26	10465.0
27	12579.0
28	14693.0
29	17502.5
30	20312.0
31	23827.0
32	27342.0
33	31121.5
34	34901.0
35	39470.5
36	44040.0
37	48578.5
38	53117.0
39	59511.5
40	65906.0
41	71580.0
42	77254.0
43	83556.5
44	89859.0
45	96603.0
46	103347.0
47	107242.5
48	111138.0
49	114001.0
50	116864.0
51	119767.5
52	122671.0
53	121998.0
54	121325.0
55	117169.5
56	113014.0
57	107531.0
58	102048.0
59	93621.5
60	85195.0
61	74949.0
62	64703.0
63	56032.0
64	47361.0
65	39708.0
66	32055.0
67	26060.5
68	20066.0
69	16081.0
70	12096.0
71	9705.5
72	7315.0
73	5940.5
74	4566.0
75	3690.0
76	2814.0
77	2244.0
78	1674.0
79	1382.0
80	1090.0
81	828.0
82	566.0
83	389.0
84	212.0
85	154.0
86	96.0
87	61.5
88	27.0
89	20.5
90	14.0
91	9.5
92	5.0
93	7.5
94	10.0
95	7.0
96	4.0
97	5.5
98	7.0
99	6.0
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.030370718708362724
2	0.004637249523212373
3	5.225069885309715E-4
4	0.0
5	6.531337356637144E-5
6	1.3062674713274289E-4
7	0.0
8	0.0
9	0.0
10	1.3062674713274289E-4
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	7.837604827964574E-4
17	0.0
18	6.531337356637144E-5
19	0.0
20	3.2656686783185724E-4
21	6.531337356637144E-5
22	0.0
23	6.531337356637144E-5
24	0.001045013977061943
25	3.918802413982287E-4
26	1.9594012069911436E-4
27	8.490738563628288E-4
28	0.0017634610862920293
29	5.878203620973431E-4
30	9.143872299292003E-4
31	5.878203620973431E-4
32	5.225069885309715E-4
33	0.0
34	9.143872299292003E-4
35	1.9594012069911436E-4
36	2.6125349426548577E-4
37	0.0015022075920265434
38	0.0
39	3.2656686783185724E-4
40	6.531337356637144E-5
41	6.531337356637145E-4
42	0.004180055908247772
43	0.0038534890404159157
44	0.004898503017477859
45	0.00267784831622123
46	0.0017634610862920293
47	9.143872299292003E-4
48	0.003657548919716801
49	0.004571936149646002
50	0.0011103273506283147
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1531080.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.2189709939337
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.296961013507	35.94553006177289
2	17.26852267738949	18.725630603069344
3	7.083690834114599	11.522112835945604
4	3.5390182439960385	7.675277100728936
5	1.922533178430989	5.211888531811248
6	1.1651901379138445	3.7905246173981006
7	0.7388324804209526	2.8041115777723804
8	0.4809432831361275	2.086099993446795
9	0.32569588593239684	1.5893006212990946
>10	1.1576022948292903	9.737383625708663
>50	0.018225469964382537	0.639489959065812
>100	0.002542412986608962	0.18313300332828822
>500	2.420873782895988E-4	0.08951746865290187
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.9594012069911436E-4
2	0.0	0.0	0.0	0.0	1.9594012069911436E-4
3	0.0	0.0	0.0	0.0	1.9594012069911436E-4
4	0.0	0.0	0.0	0.0	1.9594012069911436E-4
5	0.0	0.0	0.0	0.0	1.9594012069911436E-4
6	0.0	0.0	0.0	0.0	3.2656686783185724E-4
7	0.0	0.0	0.0	0.0	3.2656686783185724E-4
8	0.0	0.0	0.0	0.0	3.2656686783185724E-4
9	0.0	0.0	0.0	0.0	3.2656686783185724E-4
10	0.0	0.0	0.0	0.0	3.2656686783185724E-4
11	0.0	0.0	0.0	0.0	3.2656686783185724E-4
12	0.0	0.0	0.0	0.0	5.225069885309717E-4
13	0.0	0.0	0.0	0.0	6.531337356637145E-4
14	0.0	0.0	0.0	0.0	6.531337356637145E-4
15	0.0	0.0	0.0	6.531337356637146E-5	7.184471092300859E-4
16	0.0	0.0	0.0	6.531337356637146E-5	7.837604827964574E-4
17	0.0	0.0	0.0	6.531337356637146E-5	7.837604827964574E-4
18	0.0	0.0	0.0	1.306267471327429E-4	9.143872299292003E-4
19	0.0	0.0	0.0	1.9594012069911436E-4	9.143872299292003E-4
20	0.0	0.0	0.0	1.9594012069911436E-4	0.0011103273506283147
21	0.0	0.0	0.0	3.918802413982287E-4	0.0011756407241946861
22	0.0	0.0	0.0	6.531337356637145E-4	0.0011756407241946861
23	0.0	0.0	0.0	0.001306267471327429	0.0011756407241946861
24	0.0	0.0	0.0	0.0015022075920265434	0.0011756407241946861
25	0.0	0.0	0.0	0.0018940878334247721	0.0011756407241946861
26	0.0	0.0	0.0	0.002481908195522115	0.0011756407241946861
27	0.0	0.0	0.0	0.0029391018104867155	0.001306267471327429
28	0.0	0.0	0.0	0.0037228622932831724	0.001306267471327429
29	0.0	0.0	0.0	0.007249784465867231	0.001306267471327429
30	0.0	0.0	0.0	0.012997361339707919	0.001306267471327429
31	0.0	0.0	0.0	0.020573712673407008	0.0013715808448938004
32	0.0	0.0	0.0	0.03095853907046007	0.0013715808448938004
33	0.0	0.0	0.0	0.040820858478982154	0.0015022075920265434
34	0.0	0.0	0.0	0.05172819186456619	0.0016328343391592863
35	0.0	0.0	0.0	0.06309271886511482	0.0016328343391592863
36	0.0	0.0	0.0	0.08033544948663689	0.0016328343391592863
37	0.0	0.0	0.0	0.10691799252815007	0.0016328343391592863
38	0.0	0.0	0.0	0.14316691485748623	0.0016328343391592863
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATAACGC	40	7.0354063E-4	27.498388	11
GTATCAA	1060	0.0	25.123222	1
GGTATCA	510	0.0	20.71414	1
ATCAACG	1540	0.0	16.856153	3
TCAACGC	1540	0.0	16.856153	4
AACGCAG	1575	0.0	16.621248	6
CAACGCA	1565	0.0	16.586887	5
TATCAAC	1645	0.0	16.048216	2
CGCAATA	85	0.002979644	15.530024	36
GTCCTAG	330	0.0	15.339447	1
ATAAGAC	170	7.599192E-7	14.23446	3
GCACCGT	155	4.5710276E-6	14.192717	6
CCCTTAA	210	3.9963197E-8	13.624477	1
GCATAAG	130	2.3232834E-4	13.5438595	1
TAAGACC	245	1.5352271E-9	13.468597	4
TGCACCG	215	5.472066E-8	13.301546	5
TATAAGA	265	3.4924597E-10	13.282674	2
GCTCTAT	250	2.062734E-9	13.205263	1
TCGCGGT	100	0.008765288	13.199226	17
GTATTAT	340	0.0	12.946337	1
>>END_MODULE