FastQCFastQC Report
Thu 2 Feb 2017
SRR4062311_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062311_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1059031
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT23280.21982359345477137No Hit
TATCAACGCAGAGTACTTTTTTTTT13580.12823042951528332No Hit
GCGCAAGACGGACCAGAGCGAAAGC11300.10670131469239331No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACGA551.9605864E-413.81704816
GGTATCA6050.013.1877541
ACCGTGT655.4462307E-513.1546318
CGTTATT957.390372E-812.9983192
CGCCAGT1550.012.87173718
AAGACGG2300.012.8051135
GTATCAA17100.012.7755211
AATCGCT751.4780559E-512.66622615
GTTTTAC604.0962067E-412.664431
CGCATCG1600.012.46831613
GTCCTAA1155.329639E-912.3891161
GTATTTA1001.4440411E-712.3478191
GCGTTAT1001.4440411E-712.3478191
AAATCGC701.0906205E-412.213860514
CAAGACG2450.012.019994
GTCCTAC1302.6175258E-911.6902431
CGGACCA2300.011.5664549
CGCAAGA2550.011.5475262
TCGCCAG2000.011.39906517
GCATCGC1850.011.29690414
Produced by FastQC (version 0.11.5)