##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062311_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1059031
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.21869803622368	32.0	32.0	32.0	32.0	32.0
2	30.78513660128929	32.0	32.0	32.0	32.0	32.0
3	30.810010282985107	32.0	32.0	32.0	32.0	32.0
4	30.814267004459737	32.0	32.0	32.0	32.0	32.0
5	30.76165003668448	32.0	32.0	32.0	32.0	32.0
6	34.40788229995156	36.0	36.0	36.0	32.0	36.0
7	34.333536978615356	36.0	36.0	36.0	32.0	36.0
8	34.288621390686394	36.0	36.0	36.0	32.0	36.0
9	34.38992531852231	36.0	36.0	36.0	32.0	36.0
10	34.11084000373927	36.0	36.0	36.0	32.0	36.0
11	34.390478654543635	36.0	36.0	36.0	32.0	36.0
12	34.22281500730384	36.0	36.0	36.0	32.0	36.0
13	34.30674267325508	36.0	36.0	36.0	32.0	36.0
14	34.209562326315286	36.0	36.0	36.0	32.0	36.0
15	34.17672381639442	36.0	36.0	36.0	32.0	36.0
16	34.17996073769323	36.0	36.0	36.0	32.0	36.0
17	34.11249151346844	36.0	36.0	36.0	32.0	36.0
18	34.11877367140339	36.0	36.0	36.0	32.0	36.0
19	34.1256582668496	36.0	36.0	36.0	32.0	36.0
20	34.093069985675584	36.0	36.0	36.0	32.0	36.0
21	34.07834425998861	36.0	36.0	36.0	32.0	36.0
22	34.052142005285965	36.0	36.0	36.0	32.0	36.0
23	34.02204562472676	36.0	36.0	36.0	32.0	36.0
24	34.01400053445083	36.0	36.0	36.0	32.0	36.0
25	33.649622154592265	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	1.0
4	16.0
5	85.0
6	218.0
7	52.0
8	166.0
9	151.0
10	75.0
11	30.0
12	45.0
13	43.0
14	220.0
15	292.0
16	459.0
17	571.0
18	796.0
19	1055.0
20	1624.0
21	2300.0
22	3437.0
23	5018.0
24	6970.0
25	9499.0
26	12657.0
27	16238.0
28	21010.0
29	27205.0
30	35176.0
31	47307.0
32	66359.0
33	94784.0
34	212567.0
35	492605.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.75881475326245	17.105059394534567	11.381088175860702	25.75503767634228
2	16.883201498731385	19.39521958322199	37.5479802724949	26.173598645551728
3	19.694680563765438	22.92870214216513	27.835587929240376	29.541029364829058
4	12.966455350136036	15.39412437424274	35.283879661956504	36.355540613664715
5	14.877772320247857	36.35725620584124	33.29353534590244	15.471436128008465
6	34.604151844387566	34.944391060618514	16.519506827933064	13.931950267060856
7	30.73949713287467	30.113323766795798	19.91172694908749	19.235452151242043
8	28.025631839592098	33.151432598262794	18.994936208707223	19.827999353437885
9	27.055832812590108	14.672101933950534	18.56496086626463	39.707104387194725
10	16.294888523288137	26.69531589795957	30.96981902762807	26.039976551124223
11	37.05017399198124	21.591270141463045	21.792968454497313	19.5655874120584
12	24.5873506193641	23.744796084026635	28.396952772128287	23.270900524480982
13	29.351988245816734	19.714163072274236	24.90493218085861	26.028916501050425
14	23.596554002762318	19.60480503532308	24.993595239963433	31.80504572195117
15	25.152820838663743	27.046806434501907	22.1812178579837	25.619154868850647
16	25.628675149842124	26.14352700939704	23.45950953884551	24.76828830191533
17	23.913119523053634	26.15102734254237	24.905906900538795	25.029946233865196
18	24.58314072586782	25.500566714972145	25.7870909986378	24.12920156052223
19	25.351910329854793	25.219492046869078	25.444915033964676	23.983682589311453
20	25.76738084414019	24.509402701392283	24.8739422728858	24.849274181581727
21	26.471260934983633	24.458070906327738	24.65200855896385	24.418659599724784
22	25.77851465906427	24.568111525344264	24.96814479843314	24.685229017158328
23	24.390006492358957	24.729744926840468	25.420562160793146	25.459686420007426
24	24.4922320242103	25.06478826382575	25.445767828350323	24.997211883613627
25	24.811051580532144	24.65161379954314	25.316111154690564	25.221223465234154
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	443.0
1	443.0
2	461.0
3	479.0
4	479.0
5	479.0
6	915.5
7	1352.0
8	1352.0
9	1352.0
10	1613.0
11	1874.0
12	1874.0
13	1874.0
14	2350.5
15	2827.0
16	2827.0
17	2827.0
18	4762.5
19	6698.0
20	6698.0
21	6698.0
22	10517.0
23	14336.0
24	14336.0
25	14336.0
26	21046.0
27	27756.0
28	27756.0
29	27756.0
30	35302.5
31	42849.0
32	42849.0
33	42849.0
34	53077.5
35	63306.0
36	63306.0
37	63306.0
38	74999.5
39	86693.0
40	86693.0
41	86693.0
42	99404.5
43	112116.0
44	112116.0
45	112116.0
46	125127.5
47	138139.0
48	138139.0
49	138139.0
50	142464.5
51	146790.0
52	146790.0
53	146790.0
54	136946.5
55	127103.0
56	127103.0
57	127103.0
58	116166.5
59	105230.0
60	105230.0
61	105230.0
62	92148.0
63	79066.0
64	79066.0
65	79066.0
66	64417.5
67	49769.0
68	49769.0
69	49769.0
70	37633.0
71	25497.0
72	25497.0
73	25497.0
74	19473.0
75	13449.0
76	13449.0
77	13449.0
78	10663.0
79	7877.0
80	7877.0
81	7877.0
82	5434.0
83	2991.0
84	2991.0
85	2991.0
86	2209.0
87	1427.0
88	1427.0
89	1427.0
90	940.0
91	453.0
92	453.0
93	453.0
94	276.0
95	99.0
96	99.0
97	99.0
98	255.5
99	412.0
100	412.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.001038685364262236
2	1.8885188441131564E-4
3	8.498334798509204E-4
4	0.010764557411444992
5	0.033143505714185895
6	0.062132269971322845
7	0.11113933397605925
8	0.10651246280798202
9	0.11784357587266095
10	0.13323500445218317
11	0.14267759867274896
12	0.13351828227880014
13	0.12889141111072291
14	0.11557735325972515
15	0.1340848379320341
16	0.11812685369927793
17	0.12294257675176648
18	0.11142261180267622
19	0.09631446104977097
20	0.09291512713036729
21	0.09055447857522583
22	0.10566262932813109
23	0.08158401406568834
24	0.09149873799728242
25	0.08857153378890703
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1059031.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.664900794566705
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.8445996846672	42.41061816140726
2	15.161839574803412	17.182882707387417
3	4.849116819026179	8.2432417047415
4	1.9636294486040657	4.450754716097565
5	0.9641455505289762	2.7316605986123674
6	0.53595425765455	1.8221876904252434
7	0.33947396579171796	1.3465381015748081
8	0.2218773591651183	1.0058126836521502
9	0.1642248533128067	0.8375206518875217
>10	0.8211931074125302	8.396250423219005
>50	0.07133850248578823	2.8478732682321395
>100	0.05758493567831886	6.522199522457216
>500	0.00451974678256017	1.7456336256056662
>1k	5.0219408695113E-4	0.45682614470015154
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	2328	0.21982359345477137	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1358	0.12823042951528332	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	1130	0.10670131469239331	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.8885188441131564E-4
2	0.0	0.0	0.0	0.0	1.8885188441131564E-4
3	0.0	0.0	0.0	0.0	1.8885188441131564E-4
4	0.0	0.0	0.0	0.0	1.8885188441131564E-4
5	0.0	0.0	0.0	0.0	3.777037688226313E-4
6	0.0	0.0	0.0	0.0	3.777037688226313E-4
7	0.0	0.0	0.0	0.0	3.777037688226313E-4
8	0.0	0.0	0.0	0.0	3.777037688226313E-4
9	0.0	0.0	0.0	0.0	3.777037688226313E-4
10	0.0	0.0	0.0	0.0	3.777037688226313E-4
11	0.0	0.0	0.0	9.442594220565782E-5	3.777037688226313E-4
12	0.0	0.0	0.0	9.442594220565782E-5	3.777037688226313E-4
13	0.0	0.0	0.0	9.442594220565782E-5	3.777037688226313E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAACGA	55	1.9605864E-4	13.817048	16
GGTATCA	605	0.0	13.187754	1
ACCGTGT	65	5.4462307E-5	13.154631	8
CGTTATT	95	7.390372E-8	12.998319	2
CGCCAGT	155	0.0	12.871737	18
AAGACGG	230	0.0	12.805113	5
GTATCAA	1710	0.0	12.775521	1
AATCGCT	75	1.4780559E-5	12.666226	15
GTTTTAC	60	4.0962067E-4	12.66443	1
CGCATCG	160	0.0	12.468316	13
GTCCTAA	115	5.329639E-9	12.389116	1
GTATTTA	100	1.4440411E-7	12.347819	1
GCGTTAT	100	1.4440411E-7	12.347819	1
AAATCGC	70	1.0906205E-4	12.2138605	14
CAAGACG	245	0.0	12.01999	4
GTCCTAC	130	2.6175258E-9	11.690243	1
CGGACCA	230	0.0	11.566454	9
CGCAAGA	255	0.0	11.547526	2
TCGCCAG	200	0.0	11.399065	17
GCATCGC	185	0.0	11.296904	14
>>END_MODULE