FastQCFastQC Report
Thu 2 Feb 2017
SRR4062311_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062311_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1059031
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT10790.10188559163990477No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA8700.024.2879561
GTATCAA17450.024.0922241
CGCAATA1850.020.21769136
TACGAAT2100.019.90621441
GTAGGGG1001.1239063E-619.7986413
TAGACCG600.00741444818.3320755
TTAACGG600.00741444818.3320753
TATTAGG1456.2991603E-918.2082252
ATACGAA2300.018.17523840
CGACGGT2350.017.7860137
TACGACG2600.017.768015
TAGGACG1002.4840076E-517.5987914
TAACGCC2400.017.415474
TTAGGAC1651.6607373E-917.3321443
CAATACG2201.8189894E-1217.0012438
GGCGTTA2850.016.98369642
CTAGCGG2350.016.85070229
ATAACGC2500.016.7188513
TATCAAC25750.016.5759662
ATCGTTT2400.016.49964529
Produced by FastQC (version 0.11.5)